TRIM28 | Ubiquitylation Resource

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Plasmid

pcDNA5-FRT/TO-FLAG TRIM28

Parent Plasmid

pcDNA5D FRT/TO FLAG

Expressed

FLAG TRIM28

DU Number

DU15101

Genbank

NP_005753.1

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GCGGCCGCGATGGCGGCCTCCGCGGCGGCAGCCTCGGCAGCAGCGGCCTC
GGCCGCCTCTGGCAGCCCGGGCCCGGGCGAGGGCTCCGCTGGCGGCGAAA
AGCGCTCCACCGCCCCTTCGGCCGCAGCCTCGGCCTCTGCCTCAGCCGCG
GCGTCGTCGCCCGCGGGGGGCGGCGCCGAGGCGCTGGAGCTGCTGGAGCA
CTGCGGCGTGTGCAGAGAGCGCCTGCGACCCGAGAGGGAGCCCCGCCTGC
TGCCCTGTTTGCACTCGGCCTGTAGTGCCTGCTTAGGGCCCGCGGCCCCC
GCCGCCGCCAACAGCTCGGGGGACGGCGGGGCGGCGGGCGACGGCACCGT
GGTGGACTGTCCCGTGTGCAAGCAACAGTGCTTCTCCAAAGACATCGTGG
AGAATTATTTCATGCGTGATAGTGGCAGCAAGGCTGCCACCGACGCCCAG
GATGCGAACCAGTGCTGCACTAGCTGTGAGGATAATGCCCCAGCCACCAG
CTACTGTGTGGAGTGCTCGGAGCCTCTGTGTGAGACCTGTGTAGAGGCGC
ACCAGCGGGTGAAGTACACCAAGGACCATACTGTGCGCTCTACTGGGCCA
GCCAAGTCTCGGGATGGTGAACGTACTGTCTATTGCAACGTACACAAGCA
TGAACCCCTTGTGCTGTTTTGTGAGAGCTGTGATACTCTCACCTGCCGAG
ACTGCCAGCTCAATGCCCACAAGGACCACCAGTACCAGTTCTTAGAGGAT
GCAGTGAGGAACCAGCGCAAGCTCCTGGCCTCACTGGTGAAGCGCCTTGG
GGACAAACATGCAACATTGCAGAAGAGCACCAAGGAGGTTCGCAGCTCAA
TCCGCCAGGTGTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATG
GCCATCCTGCAGATCATGAAGGAGCTGAATAAGCGGGGCCGTGTGCTGGT
CAATGATGCCCAGAAGGTGACTGAGGGGCAGCAGGAGCGCCTGGAGCGGC
AGCACTGGACCATGACCAAGATCCAGAAGCACCAGGAGCACATTCTGCGC
TTTGCCTCTTGGGCTCTGGAGAGTGACAACAACACAGCCCTTTTGCTTTC
TAAGAAGTTGATCTACTTCCAGCTGCACCGGGCCCTCAAGATGATTGTGG
ATCCCGTGGAGCCACATGGCGAGATGAAGTTTCAGTGGGACCTCAATGCC
TGGACCAAGAGTGCCGAGGCCTTTGGCAAGATTGTGGCAGAGCGTCCTGG
CACTAACTCAACAGGCCCTGCACCCATGGCCCCTCCAAGAGCCCCAGGGC
CCCTGAGCAAGCAGGGCTCTGGCAGCAGCCAGCCCATGGAGGTGCAGGAA
GGCTATGGCTTTGGGTCAGGAGATGATCCCTACTCAAGTGCAGAGCCCCA
TGTGTCAGGTGTGAAACGGTCCCGCTCAGGTGAGGGCGAGGTGAGCGGCC
TTATGCGCAAGGTGCCACGAGTGAGCCTTGAACGCCTGGACCTGGACCTC
ACAGCTGACAGCCAGCCACCCGTCTTCAAGGTCTTCCCAGGCAGTACCAC
TGAGGACTACAACCTTATTGTTATTGAACGTGGCGCTGCCGCTGCAGCTA
CCGGCCAGCCAGGGACTGCGCCTGCAGGAACCCCTGGTGCCCCACCCCTG
GCTGGCATGGCCATTGTCAAGGAGGAGGAGACGGAGGCTGCCATTGGAGC
CCCTCCTACTGCCACTGAGGGCCCTGAGACCAAACCTGTGCTTATGGCTC
TTGCGGAGGGTCCTGGTGCTGAGGGTCCCCGCCTGGCCTCACCTAGTGGC
AGCACCAGCTCAGGGCTGGAGGTGGTGGCTCCTGAGGGTACCTCAGCCCC
AGGTGGTGGCCCGGGAACCCTGGATGACAGTGCCACCATTTGCCGTGTCT
GCCAGAAGCCAGGCGATCTGGTTATGTGCAACCAGTGTGAGTTTTGTTTC
CACCTGGACTGTCACCTGCCGGCCCTGCAGGATGTACCAGGGGAGGAGTG
GAGCTGCTCACTCTGCCATGTGCTCCCTGACCTGAAGGAGGAGGATGGCA
GCCTCAGCCTGGATGGTGCAGACAGCACTGGCGTGGTGGCCAAGCTCTCA
CCAGCCAACCAGCGGAAATGTGAGCGTGTACTGCTGGCCCTATTCTGTCA
CGAACCCTGCCGCCCCCTGCATCAGCTGGCTACCGACTCCACCTTCTCCC
TGGACCAGCCCGGTGGCACCCTGGATCTGACCCTGATCCGTGCCCGCCTC
CAGGAGAAGTTGTCACCTCCCTACAGCTCCCCACAGGAGTTTGCCCAGGA
TGTGGGCCGCATGTTCAAGCAATTCAACAAGTTAACTGAGGACAAGGCAG
ACGTGCAGTCCATCATCGGCCTGCAGCGCTTCTTCGAGACGCGCATGAAC
GAGGCCTTCGGTGACACCAAGTTCTCTGCTGTGCTGGTGGAGCCCCCGCC
GATGAGCCTGCCTGGTGCTGGCCTGAGTTCCCAGGAGCTGTCTGGTGGCC
CTGGTGATGGCCCCTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MDYKDDDDKGIPGIPGSTRAAAMAASAAAASAAAASAASGSPGPGEGSAG
GEKRSTAPSAAASASASAAASSPAGGGAEALELLEHCGVCRERLRPEREP
RLLPCLHSACSACLGPAAPAAANSSGDGGAAGDGTVVDCPVCKQQCFSKD
IVENYFMRDSGSKAATDAQDANQCCTSCEDNAPATSYCVECSEPLCETCV
EAHQRVKYTKDHTVRSTGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLT
CRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKSTKEVR
SSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERL
ERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKM
IVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRA
PGPLSKQGSGSSQPMEVQEGYGFGSGDDPYSSAEPHVSGVKRSRSGEGEV
SGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIERGAAA
AATGQPGTAPAGTPGAPPLAGMAIVKEEETEAAIGAPPTATEGPETKPVL
MALAEGPGAEGPRLASPSGSTSSGLEVVAPEGTSAPGGGPGTLDDSATIC
RVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEE
DGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDST
FSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTED
KADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPMSLPGAGLSSQELS
GGPGDGP*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter FLAG for mammalian expression.

Estimated MW of expressed protein : 90819 Daltons

100042 R 1000 1001 1047 19007

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