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Synonyms
Map3k1
Plasmid
pGEX-MEKK1 6His
Parent Plasmid
pGEX6P1
Expressed
GST-MEKK1 6His
DU Number
DU480
Genbank
L13103
Tag
GST
Species
Mouse
Sequence of Insert
View Sequence
GGATCCGTGACCGCAGTGCCCGCTGTGTTTTCCAAGCTGGTAACCATGCT
TAATGCTTCTGGCTCCACCCACTTCACCAGGATGCGCCGGCGTCTGATGG
CTATCGCGGATGAGGTAGAAATTGCCGAGGTCATCCAGCTGGGTGTGGAG
GACACTGTGGATGGGCATCAGGACAGCTTACAGGCCGTGGCCCCCACCAG
CTGTCTAGAAAACAGCTCCCTTGAGCACACAGTCCATAGAGAGAAAACTG
GAAAAGGACTAAGTGCTACGAGACTGAGTGCCAGCTCGGAGGACATTTCT
GACAGACTGGCCGGCGTCTCTGTAGGACTTCCCAGCTCAACAACAACAGA
ACAACCAAAGCCAGCGGTTCAAACAAAAGGCAGACCCCACAGTCAGTGTT
TGAACTCCTCCCCTTTGTCTCATGCTCAATTAATGTTCCCAGCACCATCA
GCCCCTTGTTCCTCTGCCCCGTCTGTCCCAGATATTTCTAAGCACAGACC
CCAGGCATTTGTTCCCTGCAAAATACCTTCCGCATCTCCTCAGACACAGC
GCAAGTTCTCTCTACAATTCCAGAGGAACTGCTCTGAACACCGAGACTCA
GACCAGCTCTCCCCAGTCTTCACTCAGTCAAGACCCCCACCCTCCAGTAA
CATACACAGGCCAAAGCCATCCCGACCCGTTCCGGGCAGTACAAGCAAAC
TAGGGGACGCCACAAAAAGTAGCATGACACTTGATCTGGGCAGTGCTTCC
AGGTGTGACGACAGCTTTGGCGGCGGCGGCAACAGTGGCAACGCCGTCAT
ACCCAGCGACGAGACAGTGTTCACGCCGGTGGAGGACAAGTGCAGGTTAG
ATGTGAACACCGAGCTCAACTCCAGCATCGAGGACCTTCTTGAAGCATCC
ATGCCTTCAAGTGACACGACAGTCACTTTCAAGTCCGAAGTCGCCGTCCT
CTCTCCGGAAAAGGCCGAAAATGACGACACCTACAAAGACGACGTCAATC
ATAATCAAAAGTGCAAAGAAAAGATGGAAGCTGAAGAGGAGGAGGCTTTA
GCGATCGCCATGGCGATGTCAGCGTCTCAGGATGCCCTCCCCATCGTCCC
TCAGCTGCAGGTGGAAAATGGAGAAGATATTATCATCATTCAGCAGGACA
CACCAGAAACTCTTCCAGGACATACCAAAGCGAAACAGCCTTACAGAGAA
GACGCTGAGTGGCTGAAAGGCCAGCAGATAGGCCTCGGAGCATTTTCTTC
CTGTTACCAAGCACAGGATGTGGGGACTGGGACTTTAATGGCTGTGAAAC
AGGTGACGTACGTCAGAAACACATCCTCCGAGCAGGAGGAGGTGGTGGAA
GCGTTGAGGGAAGAGATCCGGATGATGGGTCACCTCAACCATCCAAACAT
CATCCGGATGCTGGGGGCCACGTGCGAGAAGAGCAACTACAACCTCTTCA
TTGAGTGGATGGCGGGAGGATCTGTGGCTCACCTCTTGAGTAAATACGGA
GCTTTCAAGGAGTCAGTCGTCATTAACTACACTGAGCAGTTACTGCGTGG
CCTTTCCTATCTCCACGAGAACCAGATCATTCACAGAGACGTCAAAGGTG
CCAACCTGCTCATTGACAGCACCGGTCAGAGGCTGAGAATTGCAGACTTT
GGAGCTGCTGCCAGGTTGGCATCAAAAGGAACCGGTGCAGGAGAGTTCCA
GGGACAGTTACTGGGGACAATTGCATTCATGGCGCCTGAGGTCCTAAGAG
GTCAGCAGTATGGTAGGAGCTGTGATGTATGGAGTGTTGGCTGCGCCATT
ATAGAAATGGCTTGTGCAAAACCACCTTGGAATGCAGAAAAACACTCCAA
TCATCTCGCCTTGATATTTAAGATTGCTAGCGCAACTACTGCACCGTCCA
TCCCGTCACACCTGTCCCCGGGTCTGCGCGACGTGGCCGTGCGCTGCTTA
GAACTTCAGCCTCAGGACCGGCCTCCGTCCAGAGAGCTGCTGAAACATCC
GGTCTTCCGTACCACGCACCATCACCATCACCATTAAGCGGCCGC
TAATGCTTCTGGCTCCACCCACTTCACCAGGATGCGCCGGCGTCTGATGG
CTATCGCGGATGAGGTAGAAATTGCCGAGGTCATCCAGCTGGGTGTGGAG
GACACTGTGGATGGGCATCAGGACAGCTTACAGGCCGTGGCCCCCACCAG
CTGTCTAGAAAACAGCTCCCTTGAGCACACAGTCCATAGAGAGAAAACTG
GAAAAGGACTAAGTGCTACGAGACTGAGTGCCAGCTCGGAGGACATTTCT
GACAGACTGGCCGGCGTCTCTGTAGGACTTCCCAGCTCAACAACAACAGA
ACAACCAAAGCCAGCGGTTCAAACAAAAGGCAGACCCCACAGTCAGTGTT
TGAACTCCTCCCCTTTGTCTCATGCTCAATTAATGTTCCCAGCACCATCA
GCCCCTTGTTCCTCTGCCCCGTCTGTCCCAGATATTTCTAAGCACAGACC
CCAGGCATTTGTTCCCTGCAAAATACCTTCCGCATCTCCTCAGACACAGC
GCAAGTTCTCTCTACAATTCCAGAGGAACTGCTCTGAACACCGAGACTCA
GACCAGCTCTCCCCAGTCTTCACTCAGTCAAGACCCCCACCCTCCAGTAA
CATACACAGGCCAAAGCCATCCCGACCCGTTCCGGGCAGTACAAGCAAAC
TAGGGGACGCCACAAAAAGTAGCATGACACTTGATCTGGGCAGTGCTTCC
AGGTGTGACGACAGCTTTGGCGGCGGCGGCAACAGTGGCAACGCCGTCAT
ACCCAGCGACGAGACAGTGTTCACGCCGGTGGAGGACAAGTGCAGGTTAG
ATGTGAACACCGAGCTCAACTCCAGCATCGAGGACCTTCTTGAAGCATCC
ATGCCTTCAAGTGACACGACAGTCACTTTCAAGTCCGAAGTCGCCGTCCT
CTCTCCGGAAAAGGCCGAAAATGACGACACCTACAAAGACGACGTCAATC
ATAATCAAAAGTGCAAAGAAAAGATGGAAGCTGAAGAGGAGGAGGCTTTA
GCGATCGCCATGGCGATGTCAGCGTCTCAGGATGCCCTCCCCATCGTCCC
TCAGCTGCAGGTGGAAAATGGAGAAGATATTATCATCATTCAGCAGGACA
CACCAGAAACTCTTCCAGGACATACCAAAGCGAAACAGCCTTACAGAGAA
GACGCTGAGTGGCTGAAAGGCCAGCAGATAGGCCTCGGAGCATTTTCTTC
CTGTTACCAAGCACAGGATGTGGGGACTGGGACTTTAATGGCTGTGAAAC
AGGTGACGTACGTCAGAAACACATCCTCCGAGCAGGAGGAGGTGGTGGAA
GCGTTGAGGGAAGAGATCCGGATGATGGGTCACCTCAACCATCCAAACAT
CATCCGGATGCTGGGGGCCACGTGCGAGAAGAGCAACTACAACCTCTTCA
TTGAGTGGATGGCGGGAGGATCTGTGGCTCACCTCTTGAGTAAATACGGA
GCTTTCAAGGAGTCAGTCGTCATTAACTACACTGAGCAGTTACTGCGTGG
CCTTTCCTATCTCCACGAGAACCAGATCATTCACAGAGACGTCAAAGGTG
CCAACCTGCTCATTGACAGCACCGGTCAGAGGCTGAGAATTGCAGACTTT
GGAGCTGCTGCCAGGTTGGCATCAAAAGGAACCGGTGCAGGAGAGTTCCA
GGGACAGTTACTGGGGACAATTGCATTCATGGCGCCTGAGGTCCTAAGAG
GTCAGCAGTATGGTAGGAGCTGTGATGTATGGAGTGTTGGCTGCGCCATT
ATAGAAATGGCTTGTGCAAAACCACCTTGGAATGCAGAAAAACACTCCAA
TCATCTCGCCTTGATATTTAAGATTGCTAGCGCAACTACTGCACCGTCCA
TCCCGTCACACCTGTCCCCGGGTCTGCGCGACGTGGCCGTGCGCTGCTTA
GAACTTCAGCCTCAGGACCGGCCTCCGTCCAGAGAGCTGCTGAAACATCC
GGTCTTCCGTACCACGCACCATCACCATCACCATTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSVTAVPAVFSKLVTMLNASG
STHFTRMRRRLMAIADEVEIAEVIQLGVEDTVDGHQDSLQAVAPTSCLEN
SSLEHTVHREKTGKGLSATRLSASSEDISDRLAGVSVGLPSSTTTEQPKP
AVQTKGRPHSQCLNSSPLSHAQLMFPAPSAPCSSAPSVPDISKHRPQAFV
PCKIPSASPQTQRKFSLQFQRNCSEHRDSDQLSPVFTQSRPPPSSNIHRP
KPSRPVPGSTSKLGDATKSSMTLDLGSASRCDDSFGGGGNSGNAVIPSDE
TVFTPVEDKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEK
AENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQV
ENGEDIIIIQQDTPETLPGHTKAKQPYREDAEWLKGQQIGLGAFSSCYQA
QDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIRML
GATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYL
HENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLL
GTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLAL
IFKIASATTAPSIPSHLSPGLRDVAVRCLELQPQDRPPSRELLKHPVFRT
THHHHHH*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSVTAVPAVFSKLVTMLNASG
STHFTRMRRRLMAIADEVEIAEVIQLGVEDTVDGHQDSLQAVAPTSCLEN
SSLEHTVHREKTGKGLSATRLSASSEDISDRLAGVSVGLPSSTTTEQPKP
AVQTKGRPHSQCLNSSPLSHAQLMFPAPSAPCSSAPSVPDISKHRPQAFV
PCKIPSASPQTQRKFSLQFQRNCSEHRDSDQLSPVFTQSRPPPSSNIHRP
KPSRPVPGSTSKLGDATKSSMTLDLGSASRCDDSFGGGGNSGNAVIPSDE
TVFTPVEDKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEK
AENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQV
ENGEDIIIIQQDTPETLPGHTKAKQPYREDAEWLKGQQIGLGAFSSCYQA
QDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIRML
GATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYL
HENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLL
GTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLAL
IFKIASATTAPSIPSHLSPGLRDVAVRCLELQPQDRPPSRELLKHPVFRT
THHHHHH*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial construct to express GST N terminal and 6His C terminal fusion
100042 R 1000 1001 1047 19007
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