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Plasmid
pGEX6P1 Ufc1(mouse)(K0) C116K
Parent Plasmid
pGEX6P1
Expressed
GST-C3-UFC1 (K0) C116K
DU Number
DU66383
Genbank
Q9CR09 NM_025388.2
Tag
GST
Sequence of Insert
View Sequence
GGATCCATGGCGGACGAGGCCACTCGGCGGGTCGTGTCTGAGATCCCGGTGCTGAGGACCAACGCCGGACCCCGAGATCGGGAATTGTGGGTGCAGCGACTAAGGGAGGAATATCAGTCCCTGATCCGGTATGTGGAGAACAACAGGAATTCGGACAATGATTGGTTCCGACTGGAGTCCAACAGGGAAGGGACCCGGTGGTTTGGAAGATGCTGGTACATCCACGATTTCCTCAGATATGAGTTCGACATCGAGTTTGAAATTCCTATCACATATCCCACTACTGCTCCGGAAATTGCAGTCCCTGAGCTGGACGGGAGGACAGCAAGGATGTACAGGGGTGGCAGAATAAAGCTGACTGATCATTTCAGACCTTTGTGGGCCAGGAATGTGCCTAGGTTTGGACTAGCTCATCTCATGGCCCTGGGGCTGGGTCCTTGGCTGGCAGTGGAAGTCCCCGATCTGATCCAGAGGGGTGTGATCCAGCACAGAGAAAGATGCAGCCAATGAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMADEATRRVVSEIPVLRTNAGPRDRELWVQRLREEYQSLIRYVENNRNSDNDWFRLESNREGTRWFGRCWYIHDFLRYEFDIEFEIPITYPTTAPEIAVPELDGRTARMYRGGRIKLTDHFRPLWARNVPRFGLAHLMALGLGPWLAVEVPDLIQRGVIQHRERCSQ*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Kindly mutate Cysteine at 116 to Lysine on UFC1 K0 background. Please use 59746 construct as a template! Many thanks in advance! K0=K17R, K33R , K47R ,K60R ,K68R ,K77R ,K105R ,K108R ,K114R ,K122R ,K131R ,K156R ,K162R ,K164R
100042 R 1000 1001 1047 19007
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