UBE2S

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Synonyms
E2-EPF; E2EPF; EPF5
Plasmid
pET156P-1 UBE2S C95A/K100A
Parent Plasmid
pET156P-1
Expressed
6His UBE2S C95A/K100A
DU Number
DU65524
Genbank
NM_014501.3
Tag
6His
Species
Human
Sequence of Insert
GGATCCAACTCCAACGTGGAGAACCTACCCCCGCACATCATCCGCCTGGTGTACAAGGAGGTGACGACACTGACCGCAGACCCACCCGATGGCATCAAGGTCTTTCCCAACGAGGAGGACCTCACCGACCTCCAGGTCACCATCGAGGGCCCTGAGGGGACCCCATATGCTGGAGGTCTGTTCCGCATGAAACTCCTGCTGGGGAAGGACTTCCCTGCCTCCCCACCCAAGGGCTACTTCCTGACCAAGATCTTCCACCCGAACGTGGGCGCCAATGGCGAGATCGCCGTCAACGTGCTCGCGAGGGACTGGACGGCTGAGCTGGGCATCCGACACGTACTGCTGACCATCAAGTGCCTGCTGATCCACCCTAACCCCGAGTCTGCACTCAACGAGGAGGCGGGCCGCCTGCTCTTGGAGAACTACGAGGAGTATGCAGCTCGGGCCCGTCTGCTCACAGAGATCCACGGGGGCGCCGGCGGGCCCAGCGGCAGGGCCGAAGCCGGTCGGGCCCTGGCCAGTGGCACTGAAGCTTCCTCCACCGACCCTGGGGCCCCAGGGGGCCCGGGAGGGGCTGAGGGTCCCATGGCCAAGAAGCATGCTGGCGAGCGCGATAAGAAGCTGGCGGCCAAGAAAAAGACGGACAAGAAGCGGGCGCTGCGGCGGCTGTAGGCGGCCGC
Amino Acid Sequence
MGSSHHHHHHSSGLEVLFQGPGSNSNVENLPPHIIRLVYKEVTTLTADPP
DGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGY
FLTKIFHPNVGANGEIAVNVLARDWTAELGIRHVLLTIKCLLIHPNPESA
LNEEAGRLLLENYEEYAARARLLTEIHGGAGGPSGRAEAGRALASGTEAS
STDPGAPGGPGGAEGPMAKKHAGERDKKLAAKKKTDKKRALRRL*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Silent change at Ala145 (GCG > GCA)
UBE2S has no native initiating methionine
100042 R 1000 1001 1047 19007
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