UFM1 | Ubiquitylation Resource

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Synonyms

C13orf20, HLD14

Plasmid

pTXB1-UFM1 (MGCSSGAS-Met1-GCSSG-Ser2 - Asp80) T3C Mxe intein CBD

Parent Plasmid

pTXB1

Expressed

UFM1 (MGCSSGAS-Met1-GCSSG-Ser2-Asp80) T3C Mxe intein CBD

DU Number

DU65446

Genbank

NM_016617.4 → NP_057701.1

Tag

Intein

Species

Human

Amino Acid Sequence

View Sequence

MGCSSGASMGCSSGSKVSFKITLTSDPRLPYKVLSVPESTPFTAVLKFAA
EEFKVPAATSAIITNDGIGINPAQTAGNVFLKHGSELRIIPRDCICGDAL
VALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPVLADRLFHSGEHP
VYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAVIQR
SAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELT
DGRFYYAKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHATGLTGLNSG
LTTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALW
QLQ*

Vector Type

Bacterial

Antibiotic

Amp

Comments

additional N-terminal residues (MAS) derived from translation initiation at the pTXB1 vector derived Nde1 site. GCSSG now inserted between this Met1 and Ala2. Native UFM1 Met1 = Met9 of translation product (with GCSSG also between Met1 and Ser2 of UFM1 ORF - deriving from 29487)
Intein T3C mutant

PARENTAL 45876

100042 R 1000 1001 1047 19007

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