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ubiquitin

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Plasmid
pET15-M1-GCSSG-Q2-UBIQUITIN-6HIS TAG11
Parent Plasmid
pET156P-1
Expressed
M1-GCSSG-Q2-ubiquitin-His TAG11
DU Number
DU29979
Tag
6His
Species
Human
Amino Acid Sequence
MGCSSGQIFVKTLTG*TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
AGKQLEDGRTLSDYNIQKESTLHLVLRLRGGHHHHHH*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Proximity to upstream shine dalgarno sequence restored compared to 29924.
GCSSG inserted between M1 and Q2 of ubiquitin ORF.
C-terminal Hisx6 tag immediately follows GG (retain TAA stop codon).
100042 R 1000 1001 1047 19007
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