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Plasmid
pcDNA FRT TO GFP USP25 C178A
Expressed
GFP-USP25 C178A
DU Number
DU59618
Genbank
NM_013396.3
Tag
GFP
Sequence of Insert
View Sequence
ggatccATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAA GCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATTACGGGGATTAATG ACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAA TTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGACCCCTCAGCAGGA GGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCA GTGTGGGAAGCCAAGCAGATACAAATGTGATTGATCTCACTGGAGATGAT AAAGATGATCTTCAGAGAGCAATTGCCTTGAGTTTGGCCGAATCAAACAG GGCATTCAGGGAGACTGGAATAACTGATGAGGAACAAGCCATTAGCAGAG TTCTTGAAGCCAGCATAGCAGAGAATAAAGCATGTTTGAAGAGGACACCT ACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGATAGAAAAAGACAGGA CAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTgctTGGTTTAGTG CTGTTATTCAGTCATTATTTAATCTTTTGGAATTTAGAAGATTAGTTCTG AATTACAAGCCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGA ACATCGGAATTTGCCTTTTATGCGTGAGCTGAGGTATCTATTTGCACTTC TTGTTGGTACCAAAAGGAAGTATGTTGATCCATCAAGAGCAGTTGAAATT CTTAAGGATGCTTTCAAATCAAATGACTCACAGCAGCAAGATGTGAGTGA GTTTACACACAAATTATTAGATTGGTTAGAAGATGCCTTCCAAATGAAAG CTGAAGAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTG TTCTATGGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAATTTGA AAACACTGAAATGTTTGGTCAGTACCCACTTCAGGTCAATGGGTTCAAAG ATCTGCATGAGTGCCTAGAAGCTGCAATGATTGAAGGAGAAATTGAGTCT TTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAGCATTGGTTTACTGA ATTACCACCTGTGTTAACATTTGAATTGTCAAGATTTGAATTTAATCAGG CATTGGGAAGACCAGAAAAAATTCACAACAAATTAGAATTTCCCCAAGTT TTATATTTGGACAGATACATGCACAGAAACAGAGAAATAACAAGAATTAA GAGGGAAGAGATCAAGAGACTGAAAGATTACCTCACGGTATTACAACAAA GGCTAGAAAGATATTTAAGCTATGGTTCCGGTCCCAAACGATTCCCCTTG GTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCAAGTAAACCTGTTTG CACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGTTCCA TACCATCACAGACATTACCAAGCACAACAGAACAACAGGGAGCCCTATCT TCAGAACTGCCAAGCACATCACCTTCATCAGTTGCTGCCATTTCATCGAG ATCAGTAATACACAAACCATTTACTCAGTCCCGGATACCTCCAGATTTGC CCATGCATCCGGCACCGAGGCACATAACGGAGGAAGAACTTTCTGTGCTG GAAAGTTGTTTACATCGCTGGAGGACAGAAATAGAAAATGACACCAGAGA TTTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAATTAATGTACT CTGACAAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTT CACGAAGGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTTGATCA TCGTGAAAGCAGATGGATGAAGTACAATGATATTGCTGTGACAAAATCAT CATGGGAAGAGCTAGTGAGGGACTCTTTTGGTGGTTATAGAAATGCCAGT GCATACTGTTTAATGTACATAAATGATAAGGCACAGTTCCTAATACAAGA GGAGTTTAATAAAGAAACTGGGCAGCCCCTTGTTGGTATAGAAACATTAC CACCGGATTTGAGAGATTTTGTTGAGGAAGACAACCAACGATTTGAAAAA GAACTAGAAGAATGGGATGCACAACTTGCCCAGAAAGCTTTGCAGGAAAA GCTTTTAGCGTCTCAGAAATTGAGAGAGTCAGAGACTTCTGTGACAACAG CACAAGCAGCAGGAGACCCAGAATATCTAGAGCAGCCATCAAGAAGTGAT TTCTCAAAGCACTTGAAAGAAGAAACTATTCAAATAATTACCAAGGCATC ACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGGCAATTA AGTTGGAATATGCAAGGTTGGTTAAGTTGGCCCAAGAAGACACCCCACCA GAAACCGATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAGAACCA GGCACCAAAGAAAATTATTGAGAAAACATTACTAGAACAATTTGGAGATA GAAATTTGAGTTTTGATGAAAGGTGTCACAACATAATGAAAGTTGCTCAA GCCAAACTGGAAATGATAAAACCTGAAGAAGTAAACTTGGAGGAATATGA GGAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTATCTCA TAATTGGGCTAGAAAATTTTCAAAGAGAAAGTTATATAGATTCCTTGCTG TTCCTCATCTGTGCTTATCAGAATAACAAAGAACTCTTGTCTAAAGGCTT ATACAGAGGACATGATGAAGAATTGATATCACATTATAGAAGAGAATGTT TGCTAAAATTAAATGAGCAAGCCGCAGAACTCTTCGAATCTGGAGAGGAT CGAGAAGTAAACAATGGTTTGATTATCATGAATGAGTTTATTGTCCCATT TTTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGATATACTAGCTG TAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAA CCACACCTCCAAGAAAAGCTGACAGATTTTTTGCCAAAACTGCTTGATTG TTCTATGGAGATTAAAAGTTTCCATGAGCCACCGAAGTTACCTTCATATT CCACGCATGAACTCTGTGAGCGATTTGCCCGAATCATGTTGTCCCTCAGT CGAACTCCTGCTGATGGAAGATAAGCGGCCGC
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTAWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Please clone DU Number:49617 with a point mutation at C178 to Alanine
100042 R 1000 1001 1047 19007
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