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Plasmid
pFBDM-8His-mMBP-3Ala-Traf6 50-end R88A F118A F122A
Parent Plasmid
pFBDM-8His-mMBP-3Ala
Expressed
8His-mMBP-3Ala-Traf6 50-end R88A F118A F122A
DU Number
DU29560
Tag
6His
MBP
TrxA
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGAGGAGATCCAGGGATATGATGTAGAGTTTGACCCACCCCT
GGAAAGCAAGTATGAATGCCCCATCTGCTTGATGGCATTACGAGAAGCAG
TGCAAACGCCATGCGGCCATGCGTTCTGCAAAGCCTGCATCATAAAATCA
ATAAGGGATGCAGGTCACAAATGTCCAGTTGACAATGAAATACTGCTGGA
AAATCAACTAGCTCCAGACAATGCTGCAAAACGTGAGATTCTTTCTCTGA
TGGTGAAATGTCCAAATGAAGGTTGTTTGCACAAGATGGAACTGAGACAT
CTTGAGGATCATCAGGCACATTGTGAGTTTGCTCTTATGGATTGTCCCCA
ATGCCAGCGTCCCTTCCAAAAATTCCATATTAATATTCACATTCTGAAGG
ATTGTCCAAGGAGACAGGTTTCTTGTGACAACTGTGCTGCATCAATGGCA
TTTGAAGATAAAGAGATCCATGACCAGAACTGTCCTTTGGCAAATGTCAT
CTGTGAATACTGCAATACTATACTCATCAGAGAACAGATGCCTAATCATT
ATGATCTAGACTGCCCTACAGCCCCAATTCCATGCACATTCAGTACTTTT
GGTTGCCATGAAAAGATGCAGAGGAATCACTTGGCACGCCACCTACAAGA
GAACACCCAGTCACACATGAGAATGTTGGCCCAGGCTGTTCATAGTTTGA
GCGTTATACCCGACTCTGGGTATATCTCAGAGGTCCGGAATTTCCAGGAA
ACTATTCACCAGTTAGAGGGTCGCCTTGTAAGACAAGACCATCAAATCCG
GGAGCTGACTGCTAAAATGGAAACTCAGAGTATGTATGTAAGTGAGCTCA
AACGAACCATTCGAACCCTTGAGGACAAAGTTGCTGAAATCGAAGCACAG
CAGTGCAATGGAATTTATATTTGGAAGATTGGCAACTTTGGAATGCATTT
GAAATGTCAAGAAGAGGAGAAACCTGTTGTGATTCATAGCCCTGGATTCT
ACACTGGCAAACCCGGGTACAAACTGTGCATGCGCTTGCACCTTCAGTTA
CCGACTGCTCAGCGCTGTGCAAACTATATATCCCTTTTTGTCCACACAAT
GCAAGGAGAATATGACAGCCACCTCCCTTGGCCCTTCCAGGGTACAATAC
GCCTTACAATTCTTGATCAGTCTGAAGCACCTGTAAGGCAAAACCACGAA
GAGATAATGGATGCCAAACCAGAGCTGCTTGCTTTCCAGCGACCCACAAT
CCCACGGAACCCAAAAGGTTTTGGCTATGTAACTTTTATGCATCTGGAAG
CCCTAAGACAAAGAACTTTCATTAAGGATGACACATTATTAGTGCGCTGT
GAGGTCTCCACCCGCTTTGACATGGGTAGCCTTCGGAGGGAGGGTTTTCA
GCCACGAAGTACTGATGCAGGGGTATAGGCGGCCGC
GGAAAGCAAGTATGAATGCCCCATCTGCTTGATGGCATTACGAGAAGCAG
TGCAAACGCCATGCGGCCATGCGTTCTGCAAAGCCTGCATCATAAAATCA
ATAAGGGATGCAGGTCACAAATGTCCAGTTGACAATGAAATACTGCTGGA
AAATCAACTAGCTCCAGACAATGCTGCAAAACGTGAGATTCTTTCTCTGA
TGGTGAAATGTCCAAATGAAGGTTGTTTGCACAAGATGGAACTGAGACAT
CTTGAGGATCATCAGGCACATTGTGAGTTTGCTCTTATGGATTGTCCCCA
ATGCCAGCGTCCCTTCCAAAAATTCCATATTAATATTCACATTCTGAAGG
ATTGTCCAAGGAGACAGGTTTCTTGTGACAACTGTGCTGCATCAATGGCA
TTTGAAGATAAAGAGATCCATGACCAGAACTGTCCTTTGGCAAATGTCAT
CTGTGAATACTGCAATACTATACTCATCAGAGAACAGATGCCTAATCATT
ATGATCTAGACTGCCCTACAGCCCCAATTCCATGCACATTCAGTACTTTT
GGTTGCCATGAAAAGATGCAGAGGAATCACTTGGCACGCCACCTACAAGA
GAACACCCAGTCACACATGAGAATGTTGGCCCAGGCTGTTCATAGTTTGA
GCGTTATACCCGACTCTGGGTATATCTCAGAGGTCCGGAATTTCCAGGAA
ACTATTCACCAGTTAGAGGGTCGCCTTGTAAGACAAGACCATCAAATCCG
GGAGCTGACTGCTAAAATGGAAACTCAGAGTATGTATGTAAGTGAGCTCA
AACGAACCATTCGAACCCTTGAGGACAAAGTTGCTGAAATCGAAGCACAG
CAGTGCAATGGAATTTATATTTGGAAGATTGGCAACTTTGGAATGCATTT
GAAATGTCAAGAAGAGGAGAAACCTGTTGTGATTCATAGCCCTGGATTCT
ACACTGGCAAACCCGGGTACAAACTGTGCATGCGCTTGCACCTTCAGTTA
CCGACTGCTCAGCGCTGTGCAAACTATATATCCCTTTTTGTCCACACAAT
GCAAGGAGAATATGACAGCCACCTCCCTTGGCCCTTCCAGGGTACAATAC
GCCTTACAATTCTTGATCAGTCTGAAGCACCTGTAAGGCAAAACCACGAA
GAGATAATGGATGCCAAACCAGAGCTGCTTGCTTTCCAGCGACCCACAAT
CCCACGGAACCCAAAAGGTTTTGGCTATGTAACTTTTATGCATCTGGAAG
CCCTAAGACAAAGAACTTTCATTAAGGATGACACATTATTAGTGCGCTGT
GAGGTCTCCACCCGCTTTGACATGGGTAGCCTTCGGAGGGAGGGTTTTCA
GCCACGAAGTACTGATGCAGGGGTATAGGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHHHKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHP
DKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPAAAFQDKLY
PFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELK
AKGKSALMFNLQEPYFTWPLIAADGGYAFKYAAGKYDIKDVGVDNAGAKA
GLTFLVDLIKNKHMNADTDYSIAEHAFNHGETAMTINGPWAWSNIDTSAV
NYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGL
EAVNKDKPLGAVALKSYEEELVKDPRVAATMENAQKGEIMPNIPQMSAFW
YAVRTAVINAASGRQTVDAALAAAQTNAAAGSMEEIQGYDVEFDPPLESK
YECPICLMALREAVQTPCGHAFCKACIIKSIRDAGHKCPVDNEILLENQL
APDNAAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCPQCQR
PFQKFHINIHILKDCPRRQVSCDNCAASMAFEDKEIHDQNCPLANVICEY
CNTILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQ
SHMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDHQIRELT
AKMETQSMYVSELKRTIRTLEDKVAEIEAQQCNGIYIWKIGNFGMHLKCQ
EEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGE
YDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPELLAFQRPTIPRN
PKGFGYVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRREGFQPRS
TDAGV*
DKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPAAAFQDKLY
PFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELK
AKGKSALMFNLQEPYFTWPLIAADGGYAFKYAAGKYDIKDVGVDNAGAKA
GLTFLVDLIKNKHMNADTDYSIAEHAFNHGETAMTINGPWAWSNIDTSAV
NYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGL
EAVNKDKPLGAVALKSYEEELVKDPRVAATMENAQKGEIMPNIPQMSAFW
YAVRTAVINAASGRQTVDAALAAAQTNAAAGSMEEIQGYDVEFDPPLESK
YECPICLMALREAVQTPCGHAFCKACIIKSIRDAGHKCPVDNEILLENQL
APDNAAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCPQCQR
PFQKFHINIHILKDCPRRQVSCDNCAASMAFEDKEIHDQNCPLANVICEY
CNTILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQ
SHMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDHQIRELT
AKMETQSMYVSELKRTIRTLEDKVAEIEAQQCNGIYIWKIGNFGMHLKCQ
EEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGE
YDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPELLAFQRPTIPRN
PKGFGYVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRREGFQPRS
TDAGV*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
Parental 29541
100042 R 1000 1001 1047 19007
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