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Plasmid
pGEX6P1 Ufc1(ms) C116A
Parent Plasmid
pGEX6P1
Expressed
GST-C3-Ufc1(ms) C116A
DU Number
DU59458
Tag
GST
Species
Mouse
Sequence of Insert
View Sequence
GGATCCATGGCGGACGAGGCCACTCGGCGGGTCGTGTCTGAGATCCCGGTGCTGAAGACCAACGCCGGACCCCGAGATCGGGAATTGTGGGTGCAGCGACTAAAGGAGGAATATCAGTCCCTGATCCGGTATGTGGAGAACAACAAGAATTCGGACAATGATTGGTTCCGACTGGAGTCCAACAAGGAAGGGACCCGGTGGTTTGGAAAATGCTGGTACATCCACGATTTCCTCAAATATGAGTTCGACATCGAGTTTGAAATTCCTATCACATATCCCACTACTGCTCCGGAAATTGCAGTCCCTGAGCTGGACGGGAAGACAGCAAAGATGTACAGGGGTGGCAAAATAGCTCTGACTGATCATTTCAAACCTTTGTGGGCCAGGAATGTGCCTAAGTTTGGACTAGCTCATCTCATGGCCCTGGGGCTGGGTCCTTGGCTGGCAGTGGAAGTCCCCGATCTGATCCAGAAGGGTGTGATCCAGCACAAAGAAAAATGCAGCCAATGAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMADEATRRVVSEIPVLKTNAGPRDRELWVQRLKEEYQSLIRYVENNKNSDNDWFRLESNKEGTRWFGKCWYIHDFLKYEFDIEFEIPITYPTTAPEIAVPELDGKTAKMYRGGKIALTDHFKPLWARNVPKFGLAHLMALGLGPWLAVEVPDLIQKGVIQHKEKCSQ*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Please mutate Cysteine 116 to Alanine
100042 R 1000 1001 1047 19007
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