UFM1 | Ubiquitylation Resource

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Synonyms

Plasmid

pTXB1-UFM1 (Met1-GCSSG-Ser2-Asp80) T3C Mxe intein CBD

Parent Plasmid

pTXB1

Expressed

UFM1-(Met1-GCSSG-Ser2-Asp80) T3C Mxe intein CBD

DU Number

DU29487

Genbank

NM_016617.4 → NP_057701.1

Tag

Intein

Species

Human

Sequence of Insert

View Sequence

GCTAGCATGGGTTGCTCTTCCGGCTCGAAGGTTTCCTTTAAGATCACGCT
GACGTCGGACCCACGGCTGCCGTACAAAGTACTCAGTGTTCCTGAAAGTA
CACCTTTCACAGCAGTCTTAAAGTTTGCAGCAGAAGAATTTAAAGTTCCT
GCTGCAACAAGTGCAATTATTACCAATGATGGAATAGGAATAAATCCTGC
ACAGACTGCTGGAAATGTTTTTCTAAAACATGGTTCAGAACTGCGGATTA
TTCCTAGAGATTGCATCTGCGGAGATGCACTAGT

Amino Acid Sequence

View Sequence

MASMGCSSGSKVSFKITLTSDPRLPYKVLSVPESTPFTAVLKFAAEEFKV
PAATSAIITNDGIGINPAQTAGNVFLKHGSELRIIPRDCICGDALVALPE
GESVRIADIVPGARPNSDNAIDLKVLDRHGNPVLADRLFHSGEHPVYTVR
TVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAVIQRSAFSV
DCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFY
YAKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHATGLTGLNSGLTTNP
GVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ*

Vector Type

Bacterial

Antibiotic

Amp

Comments

additional N-terminal residues (MAS) derived from translation initiation at the pTXB1 vector derived Nde1 site. Native UFM1 Met1 = Met4 of translation product (with GCSSG inserted between native Met1 and Ser2)
Intein T3C mutant

PARENTAL 45876

100042 R 1000 1001 1047 19007

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