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Plasmid
pGEX6P1 Ufc1(mouse)C116S
Parent Plasmid
pGEX6P1
Expressed
GST-C3-Ufc1(mouse)C116S
DU Number
DU59294
Tag
GST
Sequence of Insert
View Sequence
GGGATCCATGGCGGACGAGGCCACTCGGCGGGTCGTGTCTGAGATCCCGGTGCTGAAGACCAACGCCGGACCCCGAGATCGGGAATTGTGGGTGCAGCGACTAAAGGAGGAATATCAGTCCCTGATCCGGTATGTGGAGAACAACAAGAATTCGGACAATGATTGGTTCCGACTGGAGTCCAACAAGGAAGGGACCCGGTGGTTTGGAAAATGCTGGTACATCCACGATTTCCTCAAATATGAGTTCGACATCGAGTTTGAAATTCCTATCACATATCCCACTACTGCTCCGGAAATTGCAGTCCCTGAGCTGGACGGGAAGACAGCAAAGATGTACAGGGGTGGCAAAATAAGTCTGACTGATCATTTCAAACCTTTGTGGGCCAGGAATGTGCCTAAGTTTGGACTAGCTCATCTCATGGCCCTGGGGCTGGGTCCTTGGCTGGCAGTGGAAGTCCCCGATCTGATCCAGAAGGGTGTGATCCAGCACAAAGAAAAATGCAGCCAATGAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMADEATRRVVSEIPVLKTNAGPRDRELWVQRLKEEYQSLIRYVENNKNSDNDWFRLESNKEGTRWFGKCWYIHDFLKYEFDIEFEIPITYPTTAPEIAVPELDGKTAKMYRGGKISLTDHFKPLWARNVPKFGLAHLMALGLGPWLAVEVPDLIQKGVIQHKEKCSQ*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Please mutate Cysteine 116 to Serine.Find the WT Ufc1 sequence from DU55394.
100042 R 1000 1001 1047 19007
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