This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Plasmid
pET15-UBIQUITIN-6HIS TAG34
Parent Plasmid
pET156P-1
Expressed
ubiquitin-His TAG34
DU Number
DU29180
Tag
6His
Species
Human
Sequence of Insert
View Sequence
GGATCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCT
CGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAG
ACAAGTAGGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAA
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTC
CACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGCATCACCATCACCATC
ACTAAGCGGCCGC
CGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAG
ACAAGTAGGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAA
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTC
CACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGCATCACCATCACCATC
ACTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MGSMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDK*GIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGGHHHHHH*
EDGRTLSDYNIQKESTLHLVLRLRGGHHHHHH*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N-ter 6His deleted. C-terminal Hisx6 tag immediately follows GG (retain TAA stop codon). Clone has 3 extra residues (MGS) upstream of ubiquitin Met1
100042 R 1000 1001 1047 19007
Download datasheet