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Plasmid
pet32a UFC1
Parent Plasmid
pET32a
Expressed
Trx-His-Thrombin-S-tag-enterokinase site-UFC1
DU Number
DU55772
Tag
6His
TrxA
Sequence of Insert
View Sequence
GGATCCATGGCGGATGAAGCCACGCGACGTGTTGTGTCTGAGATCCCGGTGCTGAAGACTAACGCCGGACCCCGAGATCGTGAGTTGTGGGTGCAGCGACTGAAGGAGGAATATCAGTCCCTTATCCGGTATGTGGAGAACAACAAGAATGCTGACAACGATTGGTTCCGACTGGAGTCCAACAAGGAAGGAACTCGGTGGTTTGGAAAATGCTGGTATATCCATGACCTCCTGAAATATGAGTTTGACATCGAGTTTGACATTCCTATCACATGTCCTACTACTGCCCCAGAAATTGCAGTTCCTGAGCTGGATGGAAAGACAGCAAAGATGTACAGGGGTGGCAAAATATGCCTGACGGATCATTTCAAACCTTTGTGGGCCAGGAATGTGCCCAAATTTGGACTAGCTCATCTCATGGCTCTGGGGCTGGGTCCATGGCTGGCAGTGGAAATCCCTGATCTGATTCAGAAGGGCGTCATCCAACACAAAGAGAAATGCAACCAATGAGCGGCCGC
Amino Acid Sequence
View Sequence
MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSMADEATRRVVSEIPVLKTNAGPRDRELWVQRLKEEYQSLIRYVENNKNADNDWFRLESNKEGTRWFGKCWYIHDLLKYEFDIEFDIPITCPTTAPEIAVPELDGKTAKMYRGGKICLTDHFKPLWARNVPKFGLAHLMALGLGPWLAVEIPDLIQKGVIQHKEKCNQ*
Vector Type
Bacterial
Antibiotic
Amp
Comments
kindly clone it in pET32a vector containing Trx- His6. Use full length WT UFC1 (DU 53290)
100042 R 1000 1001 1047 19007
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