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Plasmid
pFBDMb 6His TEVG mouse USP1 delta M1-V69, E222-F429 and L607-V735/mouse WDR48
Parent Plasmid
pFBDMb
Expressed
6His TEVG mouse USP1 delta M1-V69, E222-F429 and L607-V735
DU Number
DU58157
Genbank
NM_001301414.1
Tag
6His
Species
Mouse
Sequence of Insert
View Sequence
GGATCCGAAACCATGCATCACCATCACCATCACGAAAACCTGTATTTTCAGGGCAGCTGCGAGAAGAGAGAGAACTTGTTACCATTTGTGGGATTGAATAACCTTGGCAACACTTGTTATCTGAATAGTATTCTTCAGGTGTTATACTTTTGTCCTGGCTTTAAGACTGGAGTGAAGCACTTATTTAATATTATTTCAAGGAAGAAAGAAGCTCTGAAGGATGACTCTAATCAGAAGGATAAGGGAAGCTGCAAAGAAGAGTCTTTGGCAAGTTATGAGCTTATATGTAGTTTACAGTCCTTAATAATTTCAGTTGAACAGCTTCAGGCTAGTTTTCTCTTAAATCCAGAGAAATATACAGATGAACTTGCTACACAGCCAAGGCGACTGCTGAACACACTCAGGGAACTCAACCCTATGTATGAAGGATTTCTACAGCATGATGCACAGGAAGTGCTACAGTGTATTCTGGGAAACATTCAAGAAACATGCCAACTCCTAAAAAAAGAACAAGGTCAGCTCGTGTTAAGGACTCGGTGTTTGGAATGTGAAAGCTTAACAGAAAGAAGAGAAGATTTCCAGGATATTAGTGTTCCAGTGCAAGAAGATGAGCTTTCCAAGGTCGAGGAGAGCTCTGAAATTTCTCCAGAGCCAAAAACAGAAATGAAGACCTTGAGATGGGCAATTTCACAGTTTGCTTCAGTGGAGAGAATTGTAGGAGAGGATAAATACTTCTGTGAAAATTGCCATCATTATACGGAAGCAGAGCGAAGTCTCCTGTTTGATAAAATGCCTGAAGTTATCACTATTCATCTGAAGTGCTTTGCTGCTAGTGGCTTGGAGTTTGACTGTTACGGTGGTGGACTTTCCAAGATCAACACTCCTTTATTGACACCTCTTAAACTGTCACTAGAAGAATGGAGCACAAAGCCGACGAATGACAGCTATGGATTATTTGCCGTTGTGATGCACAGTGGCATTACTATTAGTAGTGGGCACTATACCGCATCTGTTAAAGTCACTGACCTTAACAGTCTAGAGGTGCAAAGCTTAAAGGAGTACGAGGGGAAGTGGTTGCTCTTTGACGATTCTGAGGTAAAAGTGACAGAAGAGAAAGACTTTCTGAATTCTCTCTCCCCTTCTACATCCCCTACGTCTACTCCCTACTTGCTATTCTATAAAAAATTATAGCGGCCGC
Amino Acid Sequence
View Sequence
MHHHHHHENLYFQGSCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKTGVKHLFNIISRKKEALKDDSNQKDKGSCKEESLASYELICSLQSLIIS
VEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGFLQHDAQEVLQCILGNIQETCQLLKKEQGQLVLRTRCLECESLTERREDFQDISVPVQEDE
LSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSL
EEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLEVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL*
VEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGFLQHDAQEVLQCILGNIQETCQLLKKEQGQLVLRTRCLECESLTERREDFQDISVPVQEDE
LSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSL
EEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLEVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
mouse WDR48 Xho/KpnI from DU58089 into DU58115
100042 R 1000 1001 1047 19007
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