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Plasmid
pcDNA FRT/TO HA USP35 BirA R118G
Parent Plasmid
pcDNA FRT/TO HA-MCS-BirA
Expressed
HA USP35 BirA R118G
DU Number
DU55250
Tag
HA
BirA
Sequence of Insert
View Sequence
ggatccATGGACAAGATCCTGGAAGCCGTGGTCACCAGCAGCTACCCCGTGTCCGTGAAGCAGGGCCTCGTGCGGAGAGTGCTGGAAGCCGCCAGACAGCCCCTGGAACGCGAGCAGTGTCTGGCCCTGCTGGCTCTGGGCGCCAGACTGTATGTGGGCGGAGCCGAGGAACTGCCCAGAAGAGTGGGCTGCCAGCTGCTGCACGTGGCCGGCAGACACCACCCCGACGTGTTCGCCGAGTTCTTCAGCGCCAGACGGGTGCTGAGACTGCTGCAGGGCGGAGCTGGCCCTCCTGGACCTAGAGCCCTGGCCTGTGTGCAGCTGGGCCTGCAGCTGCTCCCTGAGGGACCTGCCGCCGATGAGGTGTTCGCCCTGCTGCGGCGGGAAGTGCTGAGAACCGTGTGCGAAAGACCCGGCCCTGCCGCCTGTGCTCAGGTGGCCAGACTGCTGGCCAGACACCCCAGATGCGTGCCCGACGGCCCCCACAGACTGCTGTTCTGTCAGCAGCTCGTGCGGTGCCTGGGCCGGTTCAGATGTCCTGCCGAAGGCGAAGAGGGCGCCGTGGAATTCCTGGAACAGGCCCAGCAGGTCAGCGGCCTGCTGGCACAGCTGTGGCGAGCCCAGCCTGCCGCCATCCTGCCCTGCCTGAAAGAACTGTTCGCCGTGATCAGCTGCGCCGAGGAAGAACCCCCTAGCAGCGCTCTGGCCAGCGTGGTGCAGCATCTGCCTCTGGAACTGATGGACGGCGTCGTGCGGAACCTGAGCAACGACGACAGCGTGACCGACAGCCAGATGCTGACCGCCATCAGCCGGATGATCGACTGGGTGTCCTGGCCCCTGGGCAAGAACATCGACAAGTGGATCATTGCCCTGCTGAAGGGCCTGGCCGCCGTGAAGAAGTTCAGCATCCTGATCGAGGTGTCCCTGACCAAGATCGAGAAGGTGTTCAGCAAGCTGCTGTACCCCATCGTGCGGGGAGCCGCCCTGAGCGTGCTGAAGTACATGCTGCTGACCTTCCAGCACAGCCACGAGGCCTTCCATCTGCTGCTGCCCCACATCCCCCCCATGGTGGCCAGCCTGGTCAAAGAGGACAGCAACAGCGGCACCAGCTGCCTGGAACAGCTGGCCGAGCTGGTGCACTGCATGGTGTTCCGGTTCCCCGGCTTCCCCGACCTGTACGAGCCCGTGATGGAAGCCATCAAGGACCTGCACGTGCCCAACGAGGACCGGATCAAGCAGCTGCTGGGCCAGGACGCCTGGACCAGCCAGAAGTCCGAGCTGGCCGGCTTCTACCCCAGGCTGATGGCCAAGAGCGACACCGGCAAGATCGGCCTGATCAACCTGGGCAATACCTGCTACGTGAACAGCATCCTGCAGGCCCTGTTCATGGCCAGCGACTTCCGGCACTGCGTGCTGCGGCTGACCGAGAACAACAGCCAGCCCCTGATGACCAAGCTGCAGTGGCTGTTCGGCTTTCTGGAACACTCCCAGAGGCCCGCTATCAGCCCCGAGAACTTCCTGAGCGCCAGCTGGACCCCCTGGTTCAGCCCTGGCACCCAGCAGGACTGCAGCGAGTACCTGAAGTATCTGCTGGACCGGCTGCACGAGGAAGAGAAAACCGGCACCCGGATCTGCCAGAAGCTGAAGCAGAGCAGCAGCCCCAGCCCCCCTGAGGAACCTCCTGCCCCAAGCAGCACCAGCGTGGAAAAGATGTTCGGCGGAAAGATCGTGACCAGAATCTGCTGCCTGTGCTGTCTGAACGTGTCCAGCCGGGAAGAGGCCTTCACCGACCTGAGCCTGGCCTTCCCACCCCCCGAGAGATGCAGAAGGCGGAGACTGGGCAGCGTGATGCGGCCCACCGAGGACATCACCGCCAGAGAGCTGCCTCCCCCAACAAGCGCCCAGGGACCTGGCAGAGTGGGCCCCAGACGGCAGCGGAAGCACTGCATCACCGAGGATACCCCCCCCACCAGCCTGTACATCGAGGGCCTGGACAGCAAAGAGGCTGGCGGCCAGAGCAGCCAGGAAGAGAGAATCGAGCGCGAGGAAGAGGGCAAAGAGGAACGCACCGAGAAAGAGGAAGTCGGCGAGGAAGAAGAGAGCACCAGAGGCGAGGGCGAGCGCGAAAAAGAGGAAGAAGTCGAAGAGGAAGAGGAAAAGGTCGAGAAAGAGACAGAGAAAGAGGCCGAGCAGGAAAAAGAAGAGGACTCCCTGGGAGCCGGCACCCACCCCGATGCCGCCATTCCTAGCGGCGAGAGAACCTGCGGCAGCGAGGGCAGCAGAAGCGTGCTGGACCTGGTCAACTACTTCCTGTCCCCCGAGAAGCTGACCGCCGAGAACCGGTACTACTGCGAGAGCTGCGCCAGCCTGCAGGACGCCGAGAAGGTGGTGGAACTGAGCCAGGGCCCCTGCTACCTGATCCTGACCCTGCTGAGATTCAGCTTCGACCTGCGGACCATGCGGCGGAGAAAGATTCTGGACGACGTGTCCATCCCCCTGCTGCTGAGGCTGCCTCTGGCTGGCGGAAGAGGCCAGGCCTACGACCTGTGCAGCGTGGTGGTGCACAGCGGCGTGTCCAGCGAGAGCGGCCACTACTACTGCTACGCCAGAGAGGGCGCTGCCAGACCAGCCGCCTCTCTGGGCACAGCCGATAGACCCGAGCCCGAGAACCAGTGGTATCTGTTCAACGACACCAGAGTGTCCTTCAGCAGCTTCGAGAGCGTGTCCAACGTGACCTCATTCTTCCCAAAGGACACCGCCTACGTGCTGTTCTACCGGCAGCGGCCTAGAGAGGGCCCTGAGGCCGAACTGGGCAGCTCCAGAGTGCGGACCGAGCCCACCCTGCACAAGGACCTGATGGAAGCTATCTCCAAGGACAACATCCTGTATCTGCAGGAACAGGAAAAAGAGGCCAGAAGCAGAGCCGCCTACATCAGCGCCCTGCCCACCAGCCCTCACTGGGGCAGAGGCTTCGACGAGGACAAGGATGAGGACGAGGGCAGCCCTGGCGGCTGCAACCCTGCTGGCGGCAATGGCGGCGACTTCCACCGGCTGGTGTTCgcggccgc
Amino Acid Sequence
View Sequence
MYPYDVPDYAGSMDKILEAVVTSSYPVSVKQGLVRRVLEAARQPLEREQCLALLALGARLYVGGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLRLLQGGAGPPGPRALACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPGPAACAQVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQAQQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHLPLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKGLAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHEAFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGFPDLYEPVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSDTGKIGLINLGNTCYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWLFGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHPDAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQDAEKVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGGRGQAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPENQWYLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGSSRVRTEPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGRGFDEDKDEDEGSPGGCNPAGGNGGDFHRLVFAAAMKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEN*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Please clone USP35 WT into pcDNA5D FRT/TO HA-BirA R118G
100042 R 1000 1001 1047 19007
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