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Plasmid
pCAGGS puro 3FLAG mouse USP26
Parent Plasmid
pCAGGS puro 3FLAG
Expressed
3FLAG mouse USP26
DU Number
DU53288
Genbank
AF285570.1 GI:13603822
Tag
3FLAG
Species
Mouse
Sequence of Insert
View Sequence
GAATTCGCCACCATGGAGCCCATATTGATAAATGCTCAAGTCCAGATGTGGAGTGCAAAGGCAGGAATGTCCAAGTCAAGAAATGCACTCATTGAAACATGTGTAGGAAAACGAGAAGTTAAACTTATTCTCTATTTCAGCACTGGAAAGATTAAGACTTTGCAACTACACGATAATATTAAAAGTGTGGTCCTTCAAACCTATGGCGAAGACCAGAATTACCTACATTTGACTTTTAAAAATAATGATTTCTTGTTTGTTGAGAAACTCACCACCACAGATGCCAGAAGACTGAAGAGATTTCTAGACAAAACCTCTCAAGGTAGTATTCGGCCAGCCAGAAGTGATGAGAGATGTGGTGAGCCTAGCACAAGTGCACAGGAGTTGAATGGCTCTGGAAGTTCATGTGAAACAAATAGTGAGTGCTTTGAATCACCCAAAGAAAGTGAAATGTGCATGTTTCGTGAGTTGTCTTTGCTTCCATCCTCATCAACCTTTCTTCACAATGTAGGATTATTAGAAAACCAATTCATAAAGAGGAAAAGATTTTTCTCTGATTTAGCAAAAAATGAAAAACAGAGCAACCTGAAGGACAGTATCAGGGACTTTGAGGCAAATTTAGTGGTGTGTATCTCTAATGAAAAGGGAAAAGAAAGGAATGTAAGAGAAGTAGACATCAGTAAGCCAGGGTTTGGATTTCCATTTGAGACCAACTATCCTGAAGATAGTGGTGTGGATGTTCGTGATCTTAATGATCTCATTACAAAATTATTTTCTCCAGTTCTGTTGGAAACACACTGTATTGAGAACGGCCTAGAGTGGCATGAATATATGAAGACATACTTGCTTTACCCAGAGAAATTGTGGCAAGGCCTGCCTAATGTGGGAAACACCTGCTATATAAATGTTGTATTACAGTCTCTATGCTCAATACCACTGTTTATTAATGATTTATTCAACCAGGGTTTCCCATGGATTAAAGCTCCCAAAGATGATTTTAACATGCTCTTGATGCAACTGCTTGTTTTGAAAGATATTTACAACGCAAGATTTAGACAGAAGTTACTTATAGGTATTACAAAAGCCCTCCCCATATTTGGAGAGATATTTGCTGTTGACAGGCAGAATGATGCTCATGAGTTTTTAAGTCTCTGTTTAGTTCAGTTGAAGGAGACTTTCCAAAGAGTAACCATGATGTGGCAGTCTGAAAATGATTCGGGGGATTTTTACTTACTTAAAGACATTTTTGCTGATTATGCTACTATCAACAAAATGCCCGTTTGTCCTGTTACCAATAATTTTGAATTTGAGTTGCTAAGCTCCATTTTTTGTAAAGCTTGTGGCCTGACTCTTTTTAAGGGAGAACCAAGTAGATACCTTTCTATCAACATTCCCCAAGGAGGGAAAGACATGTCCATCCAGTCCACTTTAGATCTTTTCTTTAGTGCAGAGGAGCTTGAGCATAGGTGTGAAAAGTGTTTGTACAACAAATCTGTTTCATTTCACAGGTTTGGCCGGCTACCCAGGGTAATTATTGTTCATCTGAAACGCTATCACTTTAATGAGTCATGGGTAATGAAGAAGGATGAGCGGCCCATCCTTGTTTCCAAATACTTAAGGCTGTCTTGTCACTGTAGCAAAAGCACAAAACCGCCCCCACCCCTTCGCCCAGGTGAACATGTTAAGAATCTTGACTTATTAAAACCCCTTGAAGTGTTGGGTTCCGAAATACTCAAATTGCCTTTTAATTCAGTGAGGACCTCTAGATCCAAGGGTTTCGAAACTATAAACATCACATCAAACAGGGAGTCAGAAGCACAAAGTGGGAAAAGAGTCTCTGAAGTGTTGAGTGGAAAAGTGCAGCAGGAAAATTCAGGGAAAGGTGACACAGCACATATAGTTGGGTCAGAACTTACAAAGGAGACTGAGAAACTCAAGAAACATGAGGAAGAGCATAGACCCAGTGATTTAGATTCTGGTAGTATCAGGGAGGCCCAAAAGTACCAACAGGCTGAGAAATGTAACGAAGGGAGAAGTGATAAGCAGATTTCCCTAGAGGCACTTACTCAAAGCCGTCCAAAACCAATCTCCCAGGAACAGACAGAAAACCTTGGGAAAACTACACTGTCACATACCCAGGATAGTAGTCAGAGTTCACAGAGCTCATCAGATTCCAGTAAGAGCTCCCGATGCAGTGATGATCTCGATAAGAAGGCAAAGCCTACACGCAAGGTGGATCCAACAAAGTTTAATAAAAAAGAAGATAATGTTTACAGGCTTGTTAATATTATCAACCATATTGGGAACAGTCCCAATGGAGGCCACTACATCAATGATGCCTTTGACTTCAAGAGGCAGAGTTGGTTCACTTATAGTGATCTACATGTAACAAGAACCCAAGAGGACTTTGTATATAGGGGTCGGAGTTCTACTGGGTATGTCTTCTTTTACATGCATAATGATATATTTGAAGAGCTCTTGGCAAAGGAAACTCAGTCTACCAGCACATCCAAGGGTTAGCGGCCGC
Amino Acid Sequence
View Sequence
MDYKDHDGDYKDHDIDYKDDDDKGSPEFATMEPILINAQVQMWSAKAGMSKSRNALIETCVGKREVKLILYFSTGKIKTLQLHDNIKSVVLQTYGEDQNY
LHLTFKNNDFLFVEKLTTTDARRLKRFLDKTSQGSIRPARSDERCGEPSTSAQELNGSGSSCETNSECFESPKESEMCMFRELSLLPSSSTFLHNVGLLE
NQFIKRKRFFSDLAKNEKQSNLKDSIRDFEANLVVCISNEKGKERNVREVDISKPGFGFPFETNYPEDSGVDVRDLNDLITKLFSPVLLETHCIENGLEW
HEYMKTYLLYPEKLWQGLPNVGNTCYINVVLQSLCSIPLFINDLFNQGFPWIKAPKDDFNMLLMQLLVLKDIYNARFRQKLLIGITKALPIFGEIFAVDR
QNDAHEFLSLCLVQLKETFQRVTMMWQSENDSGDFYLLKDIFADYATINKMPVCPVTNNFEFELLSSIFCKACGLTLFKGEPSRYLSINIPQGGKDMSIQ
STLDLFFSAEELEHRCEKCLYNKSVSFHRFGRLPRVIIVHLKRYHFNESWVMKKDERPILVSKYLRLSCHCSKSTKPPPPLRPGEHVKNLDLLKPLEVLG
SEILKLPFNSVRTSRSKGFETINITSNRESEAQSGKRVSEVLSGKVQQENSGKGDTAHIVGSELTKETEKLKKHEEEHRPSDLDSGSIREAQKYQQAEKC
NEGRSDKQISLEALTQSRPKPISQEQTENLGKTTLSHTQDSSQSSQSSSDSSKSSRCSDDLDKKAKPTRKVDPTKFNKKEDNVYRLVNIINHIGNSPNGG
HYINDAFDFKRQSWFTYSDLHVTRTQEDFVYRGRSSTGYVFFYMHNDIFEELLAKETQSTSTSKG*
LHLTFKNNDFLFVEKLTTTDARRLKRFLDKTSQGSIRPARSDERCGEPSTSAQELNGSGSSCETNSECFESPKESEMCMFRELSLLPSSSTFLHNVGLLE
NQFIKRKRFFSDLAKNEKQSNLKDSIRDFEANLVVCISNEKGKERNVREVDISKPGFGFPFETNYPEDSGVDVRDLNDLITKLFSPVLLETHCIENGLEW
HEYMKTYLLYPEKLWQGLPNVGNTCYINVVLQSLCSIPLFINDLFNQGFPWIKAPKDDFNMLLMQLLVLKDIYNARFRQKLLIGITKALPIFGEIFAVDR
QNDAHEFLSLCLVQLKETFQRVTMMWQSENDSGDFYLLKDIFADYATINKMPVCPVTNNFEFELLSSIFCKACGLTLFKGEPSRYLSINIPQGGKDMSIQ
STLDLFFSAEELEHRCEKCLYNKSVSFHRFGRLPRVIIVHLKRYHFNESWVMKKDERPILVSKYLRLSCHCSKSTKPPPPLRPGEHVKNLDLLKPLEVLG
SEILKLPFNSVRTSRSKGFETINITSNRESEAQSGKRVSEVLSGKVQQENSGKGDTAHIVGSELTKETEKLKKHEEEHRPSDLDSGSIREAQKYQQAEKC
NEGRSDKQISLEALTQSRPKPISQEQTENLGKTTLSHTQDSSQSSQSSSDSSKSSRCSDDLDKKAKPTRKVDPTKFNKKEDNVYRLVNIINHIGNSPNGG
HYINDAFDFKRQSWFTYSDLHVTRTQEDFVYRGRSSTGYVFFYMHNDIFEELLAKETQSTSTSKG*
Vector Type
Mammalian
Antibiotic
Amp
Comments
(Residue 746 backmutated to Phe in this clone, as in AF285570.1)
100042 R 1000 1001 1047 19007
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