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Ubiquitin

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Plasmid
pTXB1 3xUbiquitin 1-76 S65A-Ubiquitin 1-75 (both copies codon opitmised)-Mxe intein/chitin binding domain
Parent Plasmid
pTXB1
Expressed
3xUbiquitin 1-76 S65A-Ubiquitin 1-75 (both copies codon opitmised)-Mxe intein/chitin binding domain
DU Number
DU53103
Genbank
codon optimised
Species
Human
Sequence of Insert
ATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTGGAACCGAGCGATACCATCGAAAACGTGAAAGCGAAAATCCAGGATAAAGAAGGCATCCCGCCGGATCAGCAGCGTCTGATCTTTGCGGGCAAACAGCTGGAAGATGGCCGCACCCTGTCTGATTATAACATCCAGAAAGAGGCGACCCTGCATCTGGTCTTAAGACTGCGTGGCGGTATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTGGAACCGAGCGATACCATCGAAAACGTGAAAGCGAAAATCCAGGATAAAGAAGGCATCCCGCCGGATCAGCAGCGTCTGATCTTTGCGGGCAAACAGCTGGAAGATGGCCGCACCCTGTCTGATTATAACATCCAGAAAGAGGCGACCCTGCATCTGGTCTTAAGACTGCGTGGCGGTATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTGGAACCGAGCGATACCATCGAAAACGTGAAAGCGAAAATCCAGGATAAAGAAGGCATCCCGCCGGATCAGCAGCGTCTGATCTTTGCGGGCAAACAGCTGGAAGATGGCCGCACCCTGTCTGATTATAACATCCAGAAAGAGGCGACCCTGCATCTGGTCTTAAGACTGCGTGGCGGTATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTGGAACCGAGCGATACCATCGAAAACGTGAAAGCGAAAATCCAGGATAAAGAAGGCATCCCGCCGGATCAGCAGCGTCTGATCTTTGCGGGCAAACAGCTGGAAGATGGCCGCACCCTGTCTGATTATAACATCCAGAAAGAGTCGACCCTGCATCTGGTCTTAAGACTGCGTGGCTGCATCACGGGAGATGCACTAGT
Amino Acid Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKEATLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE
NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKEATLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGK
QLEDGRTLSDYNIQKEATLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR
LRGCITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPVLADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEI
KPGDYAVIQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYYAKVASVTDAGVQPVYSLRVDTADHAFITNGFVSH
ATGLTGLNSGLTTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ*
Vector Type
Bacterial
Antibiotic
Amp
Comments
4 copies of Ubiquitin. First three have S65A mutation, fourth is wild-type.
100042 R 1000 1001 1047 19007
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