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Synonyms
FAAP43; PHF9; POG
Plasmid
pET15 6His TEV codon optimised mouse FancI M1-E1301 15TGS FANCL Q109-E294
Parent Plasmid
pET15 6His 3C
Expressed
6His TEV codon optimised mouse FancI M1-E1301 15TGS FANCL Q109-E294
DU Number
DU50969
Genbank
mouse FancI:codon optimised NM_145946.2 Human FANCL: NM_001114636.1 GI:167860137
Tag
6His
Sequence of Insert
View Sequence
CCATGGGCAGCAGCCATCACCATCACCATCACGAAAACCTGTATTTTCAGATGGACCTGAAGATCCTGTCCCTGGCTACCGACAAGACCACCGACAAGCTGCAAGAGTTCCTGCAGACCCTGAAGGACGACGACCTGGCTTCCCTGCTGCAGAACCAGGCTGTGAAGGGTCGTGCTGTGGGCACCCTGCTGCGTGCTGTGCTGAAGGGTTCCCCCTGCTCCGAAGAGGACGGTGCTCTGCGCCGTTACAAGATCTACTCCTGCTGCATCCAGCTGGTCGAGTCCGGCGACCTCCAGCAGGATGTGGCTTCCGAGATCATCGGCCTGCTGATGCTCGAGGTGCACCACTTCCCTGGTCCTCTGCTGGTGGACCTGGCCTCCGATTTCGTGGGTGCTGTGCGCGAGGACCGTCTGGTCAACGGCAAGTCCCTGGAACTGCTGCCCATCATCCTGACCGCTCTGGCTACCAAGAAGGAAGTCCTGGCTTGCGGCAAGGGCGACCTGAACGGCGAAGAGTACAAGCGTCAGCTGATCGACACCCTGTGCTCCGTGCGTTGGCCCCAGCGTTACATGATCCAGCTGACCTCCGTGTTCAAGGACGTGTGCCTGACCCCCGAAGAGATGAACCTGGTGGTGGCTAAGGTGCTGACCATGTTCTCCAAGCTGAACCTGCAAGAGATCCCCCCACTGGTGTACCAGCTGCTGGTCCTGTCCTCCAAGGGCTCCCGTCGTTCTGTGCTGGACGGTATCATCGCTTTCTTCCGTGAACTGGACAAGCAGCACCGCGAGGAACAGTCCTCCGACGAGCTGTCCGAGCTGATCACCGCTCCTGCTGACGAACTGTACCACGTCGAGGGCACCGTGATCCTGCACATCGTGTTCGCTATCAAGCTGGACTGCGAGCTGGGTCGCGAGCTGCTGAAGCACCTGAAGGCTGGTCAACAGGGCGACCCCTCCAAGTGCCTGTGCCCCTTCTCTATCGCTCTGCTGCTGTCCCTGACCCGTATCCAGCGTTTCGAAGAACAGGTGTTCGACCTGCTCAAGACCTCCGTCGTGAAGTCCTTCAAGGACCTGCAGCTCCTGCAGGGTTCCAAGTTCCTCCAGACTCTGGTGCCCCAGAGGACCTGCGTGTCCACCATGATCCTGGAAGTCGTGCGCAACTCCGTGCACTCCTGGGACCACGTGACCCAGGGCCTGATCGAGTTCGGTTTCATCCTGATGGACTCCTACGGTCCCAAGAAGATCCTCGACGGCAAGGCTGTCGAGATCGGCACCTCCCTGTCCAAGATGACCAACCAGCACGCCTGCAAGCTGGGTGCTAACATCCTCCTGGAAACCTTCAAGATCCACGAGATGATCCGCCAAGAAATCCTGGAACAGGTGCTGAACCGTGTCGTGACCCGTACCTCCTCCCCTATCAACCACTTCCTGGACCTGTTCTCCGACATCATTATGTACGCTCCTCTGATCCTCCAGAACTGCTCCAAAGTGACCGAGACTTTCGACTACCTGACCTTCCTGCCTCTGCAGACCGTGCAGGGACTGCTGAAGGCCGTGCAGCCCCTGCTGAAGATCTCCATGTCCATGCGCGACTCCCTGATCCTGGTGCTGCGCAAGGCTATGTTCGCTTCCCAGCTGGACGCTCGCAAGTCCGCTGTGGCTGGTTTCCTGCTGCTCCTGAAGAACTTCAAGGTGCTGGGTTCCCTGCCATCCTCCCAGTGCACCCAGTCCATCGGTGTCACCCAAGTGCGCGTGGACGTGCACTCTCGTTACTCCGCTGTCGCTAACGAAACCTTCTGCCTGGAAATCATCGACTCCCTCAAGCGTTCCCTGGGCCAGCAGGCTGACATCCGTCTGATGCTGTACGACGGTTTCTACGACGTGCTGCGTCGTAACTCTCAGCTGGCTTCCTCCATCATGCAGACTCTGTTCTCCCAGCTCAAGCAGTTCTACGAGCCCGAGCCCGACCTGCTGCCTCCTCTGAAGTTGGGAGCTTGCGTGCTGACTCAGGGTTCCCAGATCTTCCTGCAAGAACCCCTGGACCACCTCCTGTCCTGCATCCAACACTGCCTGGCTTGGTACAAGTCCCGTGTGGTGCCCCTGCAGCAAGGCGACGAGGGCGAGGAAGAGGAAGAAGAACTGTACTCCGAGCTGGACGACATGCTGGAATCCATCACCGTGCGTATGATCAAGTCCGAGTTGGAGGACTTCGAGTTGGACAAGTCCGCCGACTTCTCCCAGAACACCAACGTGGGCATCAAGAACAACATCTGCGCTTGCCTGATCATGGGCGTGTGCGAGGTGCTGATGGAGTACAACTTCTCCATCTCGAACTTCAGCAAGTCCAAGTTCGAAGAGATCCTGAGCCTGTTCACTTGCTACAAGAAGTTCAGCGACATCCTGTCCGAGAAGGCTGGCAAGGGCAAGGCTAAGATGACCTCCAAGGTGTCCGACTCTCTGCTGTCTCTCAAGTTCGTGTCTGACCTGCTGACCGCCCTGTTCCGTGACTCCATCCAGTCCCACGAGGAATCCCTGTCCGTGCTGAGATCCTCCGGCGAGTTCATGCACTACGCTGTGAACGTGACCCTCCAGAAGATCCAGCAGCTCATCCGTACCGGTCACGTGTCCGGACCTGACGGCCAGAACCCCGACAAGATCTTCCAGAACCTGTGCGACATCACCCGTGTGCTGCTGTGGCGTTACACCTCCATCCCCACCTCCGTGGAAGAGTCCGGAAAGAAGGAAAAGGGCAAGTCCATCTCCCTGCTGTGCCTGGAAGGCCTGCAAAAGACCTTCTCCGTGGTGCTCCAGTTCTACCAACCCAAGGTGCAGCAATTCCTGCAGGCTCTGGACGTGATGGGTACTGAAGAGGAAGAGGCTGGCGTCACCGTGACCCAGAGGGCTAGCTTCCAGATCCGTCAGTTCCAGCGTTCTCTGCTCAACCTGCTGTCCAGCGAAGAGGATGACTTCAACTCCAAGGAAGCCCTGCTGCTGATCGCCGTGCTGTCCACCCTGTCCCGTCTGCTGGAACCTACCTCCCCACAGTTCGTGCAGATGCTGTCCTGGACTTCCAAGATCTGCAAGGAATACTCTCAAGAGGACGCCAGCTTCTGCAAGTCTCTGATGAACCTCTTCTTCTCCCTCCACGTGCTGTACAAGTCTCCCGTGACCCTGCTGAGGGACCTGTCCCAGGACATCCACGGTCAACTGGGAGACATCGACCAGGACGTGGAAATCGAAAAGACCGACCACTTCGCTGTGGTCAACCTGCGTACCGCTGCTCCCACTGTGTGCCTGCTGGTGTTGTCCCAGGCCGAGAAGGTCCTGGAAGAGGTCGACTGGCTGATCGCTAAGATCAAGGGTTCCGCTAACCAGGAAACCCTGTCTGACAAAGTGACTCCCGAGGACGCCTCCTCCCAGGCTGTTCCTCCTACCCTGCTCATCGAGAAGGCCATCGTGATGCAGCTGGGTACTCTGGTCACCTTCTTCCACGAACTGGTGCAGACTGCTCTGCCCTCCGGTTCCTGCGTGGACACCCTCCTGAAGGGCCTGTCTAAGATCTACAGCACCCTGACCGCCTTCGTGAAGTACTACCTGCAAGTGTGCCAGTCCTCCCGTGGTATCCCCAACACCGTCGAGAAGCTGGTCAAGCTGTCCGGTTCCCACCTGACCCCTGTGTGCTACTCCTTCATCTCCTACGTGCAGAACAAGTCCTCTGACGCTCCCAAGTGCAGCGAAAAGGAAAAGGCTGCTGTCTCCACCACAATGGCTAAGGTCCTGCGCGAGACTAAGCCCATCCCCAACCTGGTGTTCGCCATCGAGCAGTACGAGAAGTTCCTGATCCAGTTGTCCAAGAAGTCCAAAGTCAACCTGATGCAGCACATGAAGCTGTCCACTTCCCGCGATTTCAAGATCAAGGGCAGCGTGCTGGACATGGTGCTCCGCGAGGATGAGACTGGATCGACCGGTTCAACCGGATCCACCGGGAGCACAGGCTCTACGGGATCGACTGGATCGACCGGTTCAACGGGGAGCACTGGTTCAACAGGTTCCACCGGAAGCACAGGAAGTACAGGGTCCACTGGAAGTATGGGACAGTTCTACTCAAGCCTTATTGAAGAGATAGGAACTCTTGGTTGGGATAAACTTGTGTATGCGGATACCTGCTTCAGTACCATCAAGTTAAAAGCAGAAGATGCTTCTGGTAGAGAGCATTTAATCACTCTCAAGTTGAAGGCAAAGTATCCTGCAGAATCACCAGATTATTTTGTGGATTTTCCTGTTCCATTTTGTGCCTCCTGGACACCTCAGGTAAATTCTCCTCAGAGCTCCTTAATAAGCATTTATAGTCAGTTTTTGGCAGCAATAGAATCACTAAAGGCATTCTGGGATGTTATGGATGAAATCGATGAGAAGACCTGGGTACTTGAGCCAGAAAAACCTCCACGGAGTGCAACAGCACGCAGAATTGCATTAGGTAATAATGTTTCCATAAATATAGAGGTAGACCCCAGGCATCCTACTATGCTTCCTGAGTGCTTCTTTCTTGGAGCTGACCATGTGGTAAAACCCCTGGGAATTAAGCTGAGCAGGAACATACATTTGTGGGACCCAGAAAATAGTGTGTTACAAAATTTGAAAGATGTTTTAGAATGAGCGGCCGC
Amino Acid Sequence
View Sequence
MGSSHHHHHHENLYFQMDLKILSLATDKTTDKLQEFLQTLKDDDLASLLQNQAVKGRAVGTLLRAVLKGSPCSEEDGALRRYKIYSCCIQLVESGDLQQD
VASEIIGLLMLEVHHFPGPLLVDLASDFVGAVREDRLVNGKSLELLPIILTALATKKEVLACGKGDLNGEEYKRQLIDTLCSVRWPQRYMIQLTSVFKDV
CLTPEEMNLVVAKVLTMFSKLNLQEIPPLVYQLLVLSSKGSRRSVLDGIIAFFRELDKQHREEQSSDELSELITAPADELYHVEGTVILHIVFAIKLDCE
LGRELLKHLKAGQQGDPSKCLCPFSIALLLSLTRIQRFEEQVFDLLKTSVVKSFKDLQLLQGSKFLQTLVPQRTCVSTMILEVVRNSVHSWDHVTQGLIE
FGFILMDSYGPKKILDGKAVEIGTSLSKMTNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRTSSPINHFLDLFSDIIMYAPLILQNCSKVTETF
DYLTFLPLQTVQGLLKAVQPLLKISMSMRDSLILVLRKAMFASQLDARKSAVAGFLLLLKNFKVLGSLPSSQCTQSIGVTQVRVDVHSRYSAVANETFCL
EIIDSLKRSLGQQADIRLMLYDGFYDVLRRNSQLASSIMQTLFSQLKQFYEPEPDLLPPLKLGACVLTQGSQIFLQEPLDHLLSCIQHCLAWYKSRVVPL
QQGDEGEEEEEELYSELDDMLESITVRMIKSELEDFELDKSADFSQNTNVGIKNNICACLIMGVCEVLMEYNFSISNFSKSKFEEILSLFTCYKKFSDIL
SEKAGKGKAKMTSKVSDSLLSLKFVSDLLTALFRDSIQSHEESLSVLRSSGEFMHYAVNVTLQKIQQLIRTGHVSGPDGQNPDKIFQNLCDITRVLLWRY
TSIPTSVEESGKKEKGKSISLLCLEGLQKTFSVVLQFYQPKVQQFLQALDVMGTEEEEAGVTVTQRASFQIRQFQRSLLNLLSSEEDDFNSKEALLLIAV
LSTLSRLLEPTSPQFVQMLSWTSKICKEYSQEDASFCKSLMNLFFSLHVLYKSPVTLLRDLSQDIHGQLGDIDQDVEIEKTDHFAVVNLRTAAPTVCLLV
LSQAEKVLEEVDWLIAKIKGSANQETLSDKVTPEDASSQAVPPTLLIEKAIVMQLGTLVTFFHELVQTALPSGSCVDTLLKGLSKIYSTLTAFVKYYLQV
CQSSRGIPNTVEKLVKLSGSHLTPVCYSFISYVQNKSSDAPKCSEKEKAAVSTTMAKVLRETKPIPNLVFAIEQYEKFLIQLSKKSKVNLMQHMKLSTSR
DFKIKGSVLDMVLREDETGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSMGQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDAS
GREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQVNSPQSSLISIYSQFLAAIESLKAFWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIE
VDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENSVLQNLKDVLE*
VASEIIGLLMLEVHHFPGPLLVDLASDFVGAVREDRLVNGKSLELLPIILTALATKKEVLACGKGDLNGEEYKRQLIDTLCSVRWPQRYMIQLTSVFKDV
CLTPEEMNLVVAKVLTMFSKLNLQEIPPLVYQLLVLSSKGSRRSVLDGIIAFFRELDKQHREEQSSDELSELITAPADELYHVEGTVILHIVFAIKLDCE
LGRELLKHLKAGQQGDPSKCLCPFSIALLLSLTRIQRFEEQVFDLLKTSVVKSFKDLQLLQGSKFLQTLVPQRTCVSTMILEVVRNSVHSWDHVTQGLIE
FGFILMDSYGPKKILDGKAVEIGTSLSKMTNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRTSSPINHFLDLFSDIIMYAPLILQNCSKVTETF
DYLTFLPLQTVQGLLKAVQPLLKISMSMRDSLILVLRKAMFASQLDARKSAVAGFLLLLKNFKVLGSLPSSQCTQSIGVTQVRVDVHSRYSAVANETFCL
EIIDSLKRSLGQQADIRLMLYDGFYDVLRRNSQLASSIMQTLFSQLKQFYEPEPDLLPPLKLGACVLTQGSQIFLQEPLDHLLSCIQHCLAWYKSRVVPL
QQGDEGEEEEEELYSELDDMLESITVRMIKSELEDFELDKSADFSQNTNVGIKNNICACLIMGVCEVLMEYNFSISNFSKSKFEEILSLFTCYKKFSDIL
SEKAGKGKAKMTSKVSDSLLSLKFVSDLLTALFRDSIQSHEESLSVLRSSGEFMHYAVNVTLQKIQQLIRTGHVSGPDGQNPDKIFQNLCDITRVLLWRY
TSIPTSVEESGKKEKGKSISLLCLEGLQKTFSVVLQFYQPKVQQFLQALDVMGTEEEEAGVTVTQRASFQIRQFQRSLLNLLSSEEDDFNSKEALLLIAV
LSTLSRLLEPTSPQFVQMLSWTSKICKEYSQEDASFCKSLMNLFFSLHVLYKSPVTLLRDLSQDIHGQLGDIDQDVEIEKTDHFAVVNLRTAAPTVCLLV
LSQAEKVLEEVDWLIAKIKGSANQETLSDKVTPEDASSQAVPPTLLIEKAIVMQLGTLVTFFHELVQTALPSGSCVDTLLKGLSKIYSTLTAFVKYYLQV
CQSSRGIPNTVEKLVKLSGSHLTPVCYSFISYVQNKSSDAPKCSEKEKAAVSTTMAKVLRETKPIPNLVFAIEQYEKFLIQLSKKSKVNLMQHMKLSTSR
DFKIKGSVLDMVLREDETGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSMGQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDAS
GREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQVNSPQSSLISIYSQFLAAIESLKAFWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIE
VDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENSVLQNLKDVLE*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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