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Plasmid
pcDNA5D FRT/TO BirA R118G-USP26-HA
Parent Plasmid
pcDNA5D FRT/TO BirA R118G MCS HA
Expressed
BirA R118G-USP26-HA
DU Number
DU28137
Genbank
NM_031907.1 Gene 83844
Tag
HA
BirA
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGCTGCCCTATTCCTACGTGGTTTTGTCCAAATAGGGAACTGCAAGACTGGGATATCTAAGTCAAAAGAAGCATTCATTGAAGCAGTGGAAA
GAAAGAAGAAAGATAGACTGGTGCTGTATTTCAAAAGTGGAAAATATAGCACTTTTCGGCTAAGTGATAATATTCAAAATGTAGTCCTTAAATCCTATAG
AGGAAACCAAAATCACCTGCATTTAACTTTACAAAATAATAATGGCTTGTTTATTGAAGGATTATCCTCCACAGATGCTGAACAATTGAAGATATTCTTG
GACAGAGTTCATCAAAACGAGGTTCAGCCACCTGTGAGACCTGGTAAGGGTGGGAGTGTCTTTTCTAGCACAACACAGAAGGAAATCAACAAAACTTCAT
TCCACAAAGTTGATGAGAAATCAAGTAGCAAATCTTTTGAGATAGCAAAAGGAAGTGGGACAGGTGTCCTTCAGAGGATGCCTTTGCTTACATCAAAATT
GACACTTACTTGCGGAGAGTTATCAGAAAATCAGCACAAGAAGAGGAAAAGAATGCTCTCATCTAGCTCAGAGATGAATGAGGAATTCTTGAAAGAAAAT
AATTCTGTAGAATACAAGAAATCCAAGGCAGATTGTTCGAGGTGTGTAAGCTATAATCGAGAGAAACAATTGAAGTTAAAAGAGTTAGAAGAGAATAAGA
AATTGGAATGTGAATCTTCATGCATCATGAACGCCACTGGAAATCCTTACCTAGATGACATTGGTCTTCTCCAAGCTCTCACTGAGAAAATGGTTTTGGT
ATTTCTGTTACAACAAGGGTATAGTGACGGTTACACAAAGTGGGATAAATTAAAACTATTTTTTGAATTATTTCCAGAGAAAATATGCCACGGCCTCCCC
AATTTGGGAAACACCTGTTATATGAATGCAGTGTTACAGTCTCTACTTTCAATCCCATCGTTTGCTGATGATTTACTTAATCAGAGTTTCCCATGGGGTA
AAATTCCCCTTAATGCTCTTACCATGTGCTTGGCACGGCTACTTTTTTTTAAAGATACCTATAATATAGAAATCAAGGAGATGTTACTCTTGAATCTTAA
AAAGGCCATTTCAGCAGCTGCAGAGATATTCCATGGCAATGCACAGAACGATGCTCATGAGTTTTTAGCTCACTGTTTAGATCAACTGAAAGATAACATG
GAAAAACTCAACACAATTTGGAAGCCTAAAAGTGAATTTGGGGAAGATAATTTTCCTAAACAGGTTTTTGCTGATGATCCTGACACCAGTGGGTTTTCTT
GCCCTGTCATTACTAATTTTGAGTTAGAGTTGTTGCACTCCATTGCTTGTAAAGCTTGTGGTCAGGTTATTCTCAAGACAGAACTGAATAATTACCTCTC
CATCAACCTTCCCCAAAGAATAAAAGCACATCCTTCATCTATTCAGTCTACTTTTGATCTTTTTTTTGGAGCAGAAGAGCTTGAGTATAAATGTGCAAAA
TGTGAGCACAAGACTTCCGTTGGAGTGCACTCATTCAGTAGGCTACCTAGAATCCTTATTGTTCACCTCAAACGCTATAGCTTGAATGAGTTTTGTGCAT
TAAAGAAGAATGACCAGGAAGTCATCATTTCCAAATATTTAAAGGTGTCTTCTCATTGCAATGAAGGCACCAGACCACCTCTTCCCTTGAGTGAGGATGG
AGAAATTACAGATTTCCAATTATTAAAAGTTATTCGAAAGATGACTTCTGGAAACATCAGTGTATCATGGCCTGCAACAAAGGAATCCAAAGATATCCTG
GCTCCACACATTGGATCAGATAAGGAGTCTGAACAAAAAAAAGGCCAGACAGTCTTTAAAGGGGCAAGCAGAAGACAGCAGCAAAAGTACCTTGGAAAAA
ATTCTAAACCAAATGAGCTAGAATCTGTATACTCAGGAGATCGAGCATTCATTGAAAAAGAACCGTTAGCTCACTTAATGACGTATCTGGAAGATACCTC
ACTTTGTCAGTTCCACAAAGCTGGAGGTAAACCTGCCAGCAGCCCAGGCACACCTCTCTCAAAAGTTGACTTTCAAACAGTGCCCGAAAATCCAAAACGA
AAGAAATATGTGAAAACCAGTAAGTTTGTAGCTTTTGATAGGATTATCAATCCTACTAAAGATTTGTATGAAGATAAAAATATCAGAATTCCAGAAAGAT
TCCAAAAAGTGTCTGAACAGACTCAGCAGTGTGACGGTATGAGAATCTGTGAACAAGCCCCTCAGCAGGCACTGCCTCAAAGCTTTCCAAAGCCAGGCAC
CCAGGGGCACACAAAGAACCTCCTAAGACCTACAAAATTAAATCTACAGAAGTCTAACAGGAATTCCCTACTTGCACTGGGTTCCAATAAGAATCCAAGA
AACAAAGACATTTTAGATAAGATAAAATCTAAAGCCAAGGAAACAAAAAGAAATGATGATAAGGGAGATCATACCTACCGGCTCATTAGTGTTGTCAGCC
ATCTTGGGAAGACTCTAAAGTCAGGCCATTATATCTGTGATGCCTATGACTTTGAGAAACAGATCTGGTTCACTTACGATGATATGCGGGTGTTAGGTAT
CCAGGAGGCCCAGATGCAGGAGGATAGGCGTTGCACTGGGTACATCTTCTTTTACATGCATAATGAGATCTTTGAAGAGATGTTGAAAAGAGAAGAGAAT
GCCCAGCTTAATAGCAAGGAGGTAGAGGAGACCCTTCAGAAGGAAGCGGCCGC
GAAAGAAGAAAGATAGACTGGTGCTGTATTTCAAAAGTGGAAAATATAGCACTTTTCGGCTAAGTGATAATATTCAAAATGTAGTCCTTAAATCCTATAG
AGGAAACCAAAATCACCTGCATTTAACTTTACAAAATAATAATGGCTTGTTTATTGAAGGATTATCCTCCACAGATGCTGAACAATTGAAGATATTCTTG
GACAGAGTTCATCAAAACGAGGTTCAGCCACCTGTGAGACCTGGTAAGGGTGGGAGTGTCTTTTCTAGCACAACACAGAAGGAAATCAACAAAACTTCAT
TCCACAAAGTTGATGAGAAATCAAGTAGCAAATCTTTTGAGATAGCAAAAGGAAGTGGGACAGGTGTCCTTCAGAGGATGCCTTTGCTTACATCAAAATT
GACACTTACTTGCGGAGAGTTATCAGAAAATCAGCACAAGAAGAGGAAAAGAATGCTCTCATCTAGCTCAGAGATGAATGAGGAATTCTTGAAAGAAAAT
AATTCTGTAGAATACAAGAAATCCAAGGCAGATTGTTCGAGGTGTGTAAGCTATAATCGAGAGAAACAATTGAAGTTAAAAGAGTTAGAAGAGAATAAGA
AATTGGAATGTGAATCTTCATGCATCATGAACGCCACTGGAAATCCTTACCTAGATGACATTGGTCTTCTCCAAGCTCTCACTGAGAAAATGGTTTTGGT
ATTTCTGTTACAACAAGGGTATAGTGACGGTTACACAAAGTGGGATAAATTAAAACTATTTTTTGAATTATTTCCAGAGAAAATATGCCACGGCCTCCCC
AATTTGGGAAACACCTGTTATATGAATGCAGTGTTACAGTCTCTACTTTCAATCCCATCGTTTGCTGATGATTTACTTAATCAGAGTTTCCCATGGGGTA
AAATTCCCCTTAATGCTCTTACCATGTGCTTGGCACGGCTACTTTTTTTTAAAGATACCTATAATATAGAAATCAAGGAGATGTTACTCTTGAATCTTAA
AAAGGCCATTTCAGCAGCTGCAGAGATATTCCATGGCAATGCACAGAACGATGCTCATGAGTTTTTAGCTCACTGTTTAGATCAACTGAAAGATAACATG
GAAAAACTCAACACAATTTGGAAGCCTAAAAGTGAATTTGGGGAAGATAATTTTCCTAAACAGGTTTTTGCTGATGATCCTGACACCAGTGGGTTTTCTT
GCCCTGTCATTACTAATTTTGAGTTAGAGTTGTTGCACTCCATTGCTTGTAAAGCTTGTGGTCAGGTTATTCTCAAGACAGAACTGAATAATTACCTCTC
CATCAACCTTCCCCAAAGAATAAAAGCACATCCTTCATCTATTCAGTCTACTTTTGATCTTTTTTTTGGAGCAGAAGAGCTTGAGTATAAATGTGCAAAA
TGTGAGCACAAGACTTCCGTTGGAGTGCACTCATTCAGTAGGCTACCTAGAATCCTTATTGTTCACCTCAAACGCTATAGCTTGAATGAGTTTTGTGCAT
TAAAGAAGAATGACCAGGAAGTCATCATTTCCAAATATTTAAAGGTGTCTTCTCATTGCAATGAAGGCACCAGACCACCTCTTCCCTTGAGTGAGGATGG
AGAAATTACAGATTTCCAATTATTAAAAGTTATTCGAAAGATGACTTCTGGAAACATCAGTGTATCATGGCCTGCAACAAAGGAATCCAAAGATATCCTG
GCTCCACACATTGGATCAGATAAGGAGTCTGAACAAAAAAAAGGCCAGACAGTCTTTAAAGGGGCAAGCAGAAGACAGCAGCAAAAGTACCTTGGAAAAA
ATTCTAAACCAAATGAGCTAGAATCTGTATACTCAGGAGATCGAGCATTCATTGAAAAAGAACCGTTAGCTCACTTAATGACGTATCTGGAAGATACCTC
ACTTTGTCAGTTCCACAAAGCTGGAGGTAAACCTGCCAGCAGCCCAGGCACACCTCTCTCAAAAGTTGACTTTCAAACAGTGCCCGAAAATCCAAAACGA
AAGAAATATGTGAAAACCAGTAAGTTTGTAGCTTTTGATAGGATTATCAATCCTACTAAAGATTTGTATGAAGATAAAAATATCAGAATTCCAGAAAGAT
TCCAAAAAGTGTCTGAACAGACTCAGCAGTGTGACGGTATGAGAATCTGTGAACAAGCCCCTCAGCAGGCACTGCCTCAAAGCTTTCCAAAGCCAGGCAC
CCAGGGGCACACAAAGAACCTCCTAAGACCTACAAAATTAAATCTACAGAAGTCTAACAGGAATTCCCTACTTGCACTGGGTTCCAATAAGAATCCAAGA
AACAAAGACATTTTAGATAAGATAAAATCTAAAGCCAAGGAAACAAAAAGAAATGATGATAAGGGAGATCATACCTACCGGCTCATTAGTGTTGTCAGCC
ATCTTGGGAAGACTCTAAAGTCAGGCCATTATATCTGTGATGCCTATGACTTTGAGAAACAGATCTGGTTCACTTACGATGATATGCGGGTGTTAGGTAT
CCAGGAGGCCCAGATGCAGGAGGATAGGCGTTGCACTGGGTACATCTTCTTTTACATGCATAATGAGATCTTTGAAGAGATGTTGAAAAGAGAAGAGAAT
GCCCAGCTTAATAGCAAGGAGGTAGAGGAGACCCTTCAGAAGGAAGCGGCCGC
Amino Acid Sequence
View Sequence
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMAALFLRGFVQIGNCKTGISKSKEAFIEAVERKKKDRLVLYFKSGKYSTFRLSDNIQNVVLKSYRGNQNHLHLTLQN
NNGLFIEGLSSTDAEQLKIFLDRVHQNEVQPPVRPGKGGSVFSSTTQKEINKTSFHKVDEKSSSKSFEIAKGSGTGVLQRMPLLTSKLTLTCGELSENQH
KKRKRMLSSSSEMNEEFLKENNSVEYKKSKADCSRCVSYNREKQLKLKELEENKKLECESSCIMNATGNPYLDDIGLLQALTEKMVLVFLLQQGYSDGYT
KWDKLKLFFELFPEKICHGLPNLGNTCYMNAVLQSLLSIPSFADDLLNQSFPWGKIPLNALTMCLARLLFFKDTYNIEIKEMLLLNLKKAISAAAEIFHG
NAQNDAHEFLAHCLDQLKDNMEKLNTIWKPKSEFGEDNFPKQVFADDPDTSGFSCPVITNFELELLHSIACKACGQVILKTELNNYLSINLPQRIKAHPS
SIQSTFDLFFGAEELEYKCAKCEHKTSVGVHSFSRLPRILIVHLKRYSLNEFCALKKNDQEVIISKYLKVSSHCNEGTRPPLPLSEDGEITDFQLLKVIR
KMTSGNISVSWPATKESKDILAPHIGSDKESEQKKGQTVFKGASRRQQQKYLGKNSKPNELESVYSGDRAFIEKEPLAHLMTYLEDTSLCQFHKAGGKPA
SSPGTPLSKVDFQTVPENPKRKKYVKTSKFVAFDRIINPTKDLYEDKNIRIPERFQKVSEQTQQCDGMRICEQAPQQALPQSFPKPGTQGHTKNLLRPTK
LNLQKSNRNSLLALGSNKNPRNKDILDKIKSKAKETKRNDDKGDHTYRLISVVSHLGKTLKSGHYICDAYDFEKQIWFTYDDMRVLGIQEAQMQEDRRCT
GYIFFYMHNEIFEEMLKREENAQLNSKEVEETLQKEAAAYPYDVPDYA*
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMAALFLRGFVQIGNCKTGISKSKEAFIEAVERKKKDRLVLYFKSGKYSTFRLSDNIQNVVLKSYRGNQNHLHLTLQN
NNGLFIEGLSSTDAEQLKIFLDRVHQNEVQPPVRPGKGGSVFSSTTQKEINKTSFHKVDEKSSSKSFEIAKGSGTGVLQRMPLLTSKLTLTCGELSENQH
KKRKRMLSSSSEMNEEFLKENNSVEYKKSKADCSRCVSYNREKQLKLKELEENKKLECESSCIMNATGNPYLDDIGLLQALTEKMVLVFLLQQGYSDGYT
KWDKLKLFFELFPEKICHGLPNLGNTCYMNAVLQSLLSIPSFADDLLNQSFPWGKIPLNALTMCLARLLFFKDTYNIEIKEMLLLNLKKAISAAAEIFHG
NAQNDAHEFLAHCLDQLKDNMEKLNTIWKPKSEFGEDNFPKQVFADDPDTSGFSCPVITNFELELLHSIACKACGQVILKTELNNYLSINLPQRIKAHPS
SIQSTFDLFFGAEELEYKCAKCEHKTSVGVHSFSRLPRILIVHLKRYSLNEFCALKKNDQEVIISKYLKVSSHCNEGTRPPLPLSEDGEITDFQLLKVIR
KMTSGNISVSWPATKESKDILAPHIGSDKESEQKKGQTVFKGASRRQQQKYLGKNSKPNELESVYSGDRAFIEKEPLAHLMTYLEDTSLCQFHKAGGKPA
SSPGTPLSKVDFQTVPENPKRKKYVKTSKFVAFDRIINPTKDLYEDKNIRIPERFQKVSEQTQQCDGMRICEQAPQQALPQSFPKPGTQGHTKNLLRPTK
LNLQKSNRNSLLALGSNKNPRNKDILDKIKSKAKETKRNDDKGDHTYRLISVVSHLGKTLKSGHYICDAYDFEKQIWFTYDDMRVLGIQEAQMQEDRRCT
GYIFFYMHNEIFEEMLKREENAQLNSKEVEETLQKEAAAYPYDVPDYA*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Parental 49108
100042 R 1000 1001 1047 19007
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