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Plasmid
pET28a 6His-Halo.TEV-ubiquitin dimer
Parent Plasmid
pET28a 6His-Halo.TEV
Expressed
6His-HALO-ubiquitin dimer
DU Number
DU24951
Genbank
P62988
Tag
6His
HALO
Species
Human
Sequence of Insert
View Sequence
GGATCCGCCGGCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCAT
CACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGA
TCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCT
GGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAA
AGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGCGGCATGCAGATCT
TCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGT
GACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCC
TCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGAC
GCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTC
CTCCGTCTCAGAGGCGGCTGATAAGCGGCCGC
CACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGA
TCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCT
GGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAA
AGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGCGGCATGCAGATCT
TCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGT
GACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCC
TCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGAC
GCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTC
CTCCGTCTCAGAGGCGGCTGATAAGCGGCCGC
Amino Acid Sequence
View Sequence
MHHHHHHMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPT
SSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIE
ALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPE
FARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPF
LNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTP
GVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTL
EISGENLYFQGGSAGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEG
IPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLT
GKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDY
NIQKESTLHLVLRLRGG**
SSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIE
ALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPE
FARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPF
LNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTP
GVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTL
EISGENLYFQGGSAGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEG
IPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLT
GKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDY
NIQKESTLHLVLRLRGG**
Vector Type
Bacterial
Antibiotic
Kan
Comments
Parental 45630
100042 R 1000 1001 1047 19007
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