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Plasmid
pSC-B UBA1 (yeast)
Parent Plasmid
pSC-B
DU Number
DU51208
Genbank
Z28210.1
Species
Yeast
Sequence of Insert
View Sequence
AGATCTATGAGCAGCAATAATAGTGGTCTCTCTGCCGCCGGAGAAATCGATGAAAGTCTTTATTCTCGTCAACTTTATGTGTTGGGTAAGGAAGCAATGT
TGAAAATGCAAACCTCCAACGTATTAATTCTCGGCTTGAAGGGGCTAGGTGTTGAGATAGCGAAGAACGTTGTGTTGGCAGGTGTCAAATCTATGACCGT
TTTTGACCCTGAGCCGGTTCAATTGGCAGACTTATCAACCCAATTCTTTTTGACCGAAAAGGATATTGGTCAAAAGAGAGGAGATGTGACCAGAGCCAAA
TTGGCAGAGTTGAACGCATATGTCCCGGTTAATGTGCTGGATTCCTTAGACGATGTTACCCAATTGAGTCAATTCCAGGTCGTTGTCGCTACGGACACTG
TTTCTTTGGAAGATAAGGTGAAAATCAACGAATTTTGCCATTCCTCCGGTATTAGATTCATTTCCTCGGAGACAAGAGGGCTGTTTGGTAACACCTTCGT
AGACCTAGGTGACGAGTTCACAGTGTTGGACCCAACGGGTGAAGAACCTCGCACTGGTATGGTTTCAGACATCGAGCCCGATGGAACAGTGACCATGCTA
GATGATAACAGACACGGGTTGGAAGACGGAAATTTTGTCAGGTTCTCCGAAGTAGAAGGTCTTGATAAGTTGAATGATGGGACCCTTTTTAAAGTTGAGG
TTTTGGGGCCCTTTGCATTCAGAATTGGTTCAGTCAAAGAATATGGCGAGTATAAGAAGGGCGGTATCTTCACCGAAGTCAAAGTACCCCGTAAAATCTC
ATTCAAGTCTTTGAAACAACAACTGTCCAATCCTGAGTTTGTTTTTTCGGATTTTGCTAAATTTGACAGAGCTGCTCAATTGCATTTGGGATTTCAGGCT
TTACATCAGTTTGCCGTTAGACATAATGGTGAACTGCCAAGAACAATGAATGACGAAGACGCCAATGAATTAATCAAGTTGGTCACCGATTTGTCCGTTC
AGCAACCGGAGGTTCTGGGTGAAGGTGTAGATGTTAATGAAGATTTAATCAAAGAGCTTTCCTATCAGGCAAGAGGTGACATACCGGGTGTCGTAGCATT
TTTCGGTGGCCTTGTGGCACAAGAAGTGTTAAAAGCATGTTCTGGTAAATTTACTCCGCTAAAGCAATTTATGTACTTTGATTCCTTGGAATCATTGCCA
GATCCTAAAAACTTCCCAAGAAATGAGAAGACTACTCAGCCAGTAAACTCTCGTTACGATAATCAAATCGCGGTTTTCGGTTTGGATTTTCAGAAAAAGA
TTGCCAATTCAAAGGTCTTTCTTGTCGGATCTGGTGCCATTGGTTGTGAGATGTTGAAAAATTGGGCACTTTTGGGGCTTGGTTCTGGCTCCGACGGTTA
CATCGTTGTTACGGATAACGATTCGATCGAAAAGTCCAACTTAAACCGTCAGTTCTTGTTTAGACCAAAGGATGTCGGAAAGAACAAATCTGAAGTAGCG
GCAGAGGCCGTTTGTGCTATGAACCCCGATTTGAAGGGCAAGATAAATGCTAAAATTGATAAAGTGGGGCCAGAAACTGAGGAAATATTTAATGACTCCT
TTTGGGAAAGCCTGGATTTTGTCACCAACGCTCTAGACAATGTCGACGCAAGAACATACGTAGATCGCCGTTGTGTGTTTTATAGAAAACCACTGTTAGA
ATCTGGTACCCTAGGTACTAAAGGTAATACTCAAGTCATCATTCCAAGATTGACTGAATCATACTCTTCTTCTAGAGACCCACCAGAAAAGTCTATCCCA
TTGTGTACCCTACGTTCTTTCCCAAACAAGATTGATCACACCATTGCCTGGGCCAAATCTCTTTTCCAAGGTTACTTCACTGATTCCGCCGAAAACGTTA
ATATGTACTTGACACAACCGAATTTCGTTGAGCAAACACTAAAGCAAAGCGGGGATGTCAAGGGAGTTCTAGAATCCATCTCAGATTCTCTATCTAGTAA
ACCACACAATTTTGAAGATTGTATCAAATGGGCAAGGTTAGAATTTGAAAAAAAATTCAACCATGATATCAAGCAGTTACTGTTCAACTTCCCCAAGGAT
GCCAAGACTTCCAACGGTGAACCATTTTGGTCCGGTGCTAAGCGTGCTCCAACACCATTAGAGTTTGATATTTACAATAACGATCACTTCCATTTTGTCG
TAGCAGGTGCTAGTTTACGCGCTTATAATTATGGTATTAAAAGTGATGACTCTAATTCTAAACCCAACGTGGACGAGTATAAGTCCGTCATCGATCATAT
GATAATTCCAGAATTTACTCCAAATGCCAATTTGAAAATTCAAGTTAATGACGATGATCCGGATCCAAATGCCAACGCGGCTAATGGCTCAGATGAAATT
GATCAGCTGGTATCATCCCTACCAGATCCTTCTACTTTGGCTGGCTTTAAACTGGAGCCTGTAGATTTCGAGAAAGACGATGACACTAATCATCATATTG
AATTTATAACGGCTTGTTCTAACTGTAGAGCTCAAAACTACTTCATCGAAACTGCTGATCGCCAAAAGACCAAATTCATAGCTGGCCGTATTATTCCTGC
CATCGCAACAACCACTTCTTTGGTTACTGGTTTGGTCAATTTGGAATTATATAAACTGATTGACAACAAGACAGATATTGAGCAATATAAGAATGGCTTT
GTTAATTTAGCTTTGCCATTTTTCGGTTTTTCGGAACCAATTGCTTCACCAAAGGGAGAATATAACAACAAGAAATATGATAAAATTTGGGATAGATTTG
ATATCAAGGGAGATATTAAATTAAGTGATTTAATTGAACATTTTGAAAAAGATGAAGGGTTAGAAATAACAATGCTTTCTTATGGCGTTTCTCTTCTTTA
TGCCTCATTTTTCCCTCCAAAGAAATTGAAAGAAAGACTAAATCTACCAATAACTCAATTGGTTAAATTAGTGACTAAGAAAGATATTCCGGCACATGTA
TCTACAATGATTCTCGAAATTTGCGCAGATGACAAGGAAGGAGAGGATGTTGAAGTTCCTTTCATTACCATTCATCTATGAGCGGCCGC
TGAAAATGCAAACCTCCAACGTATTAATTCTCGGCTTGAAGGGGCTAGGTGTTGAGATAGCGAAGAACGTTGTGTTGGCAGGTGTCAAATCTATGACCGT
TTTTGACCCTGAGCCGGTTCAATTGGCAGACTTATCAACCCAATTCTTTTTGACCGAAAAGGATATTGGTCAAAAGAGAGGAGATGTGACCAGAGCCAAA
TTGGCAGAGTTGAACGCATATGTCCCGGTTAATGTGCTGGATTCCTTAGACGATGTTACCCAATTGAGTCAATTCCAGGTCGTTGTCGCTACGGACACTG
TTTCTTTGGAAGATAAGGTGAAAATCAACGAATTTTGCCATTCCTCCGGTATTAGATTCATTTCCTCGGAGACAAGAGGGCTGTTTGGTAACACCTTCGT
AGACCTAGGTGACGAGTTCACAGTGTTGGACCCAACGGGTGAAGAACCTCGCACTGGTATGGTTTCAGACATCGAGCCCGATGGAACAGTGACCATGCTA
GATGATAACAGACACGGGTTGGAAGACGGAAATTTTGTCAGGTTCTCCGAAGTAGAAGGTCTTGATAAGTTGAATGATGGGACCCTTTTTAAAGTTGAGG
TTTTGGGGCCCTTTGCATTCAGAATTGGTTCAGTCAAAGAATATGGCGAGTATAAGAAGGGCGGTATCTTCACCGAAGTCAAAGTACCCCGTAAAATCTC
ATTCAAGTCTTTGAAACAACAACTGTCCAATCCTGAGTTTGTTTTTTCGGATTTTGCTAAATTTGACAGAGCTGCTCAATTGCATTTGGGATTTCAGGCT
TTACATCAGTTTGCCGTTAGACATAATGGTGAACTGCCAAGAACAATGAATGACGAAGACGCCAATGAATTAATCAAGTTGGTCACCGATTTGTCCGTTC
AGCAACCGGAGGTTCTGGGTGAAGGTGTAGATGTTAATGAAGATTTAATCAAAGAGCTTTCCTATCAGGCAAGAGGTGACATACCGGGTGTCGTAGCATT
TTTCGGTGGCCTTGTGGCACAAGAAGTGTTAAAAGCATGTTCTGGTAAATTTACTCCGCTAAAGCAATTTATGTACTTTGATTCCTTGGAATCATTGCCA
GATCCTAAAAACTTCCCAAGAAATGAGAAGACTACTCAGCCAGTAAACTCTCGTTACGATAATCAAATCGCGGTTTTCGGTTTGGATTTTCAGAAAAAGA
TTGCCAATTCAAAGGTCTTTCTTGTCGGATCTGGTGCCATTGGTTGTGAGATGTTGAAAAATTGGGCACTTTTGGGGCTTGGTTCTGGCTCCGACGGTTA
CATCGTTGTTACGGATAACGATTCGATCGAAAAGTCCAACTTAAACCGTCAGTTCTTGTTTAGACCAAAGGATGTCGGAAAGAACAAATCTGAAGTAGCG
GCAGAGGCCGTTTGTGCTATGAACCCCGATTTGAAGGGCAAGATAAATGCTAAAATTGATAAAGTGGGGCCAGAAACTGAGGAAATATTTAATGACTCCT
TTTGGGAAAGCCTGGATTTTGTCACCAACGCTCTAGACAATGTCGACGCAAGAACATACGTAGATCGCCGTTGTGTGTTTTATAGAAAACCACTGTTAGA
ATCTGGTACCCTAGGTACTAAAGGTAATACTCAAGTCATCATTCCAAGATTGACTGAATCATACTCTTCTTCTAGAGACCCACCAGAAAAGTCTATCCCA
TTGTGTACCCTACGTTCTTTCCCAAACAAGATTGATCACACCATTGCCTGGGCCAAATCTCTTTTCCAAGGTTACTTCACTGATTCCGCCGAAAACGTTA
ATATGTACTTGACACAACCGAATTTCGTTGAGCAAACACTAAAGCAAAGCGGGGATGTCAAGGGAGTTCTAGAATCCATCTCAGATTCTCTATCTAGTAA
ACCACACAATTTTGAAGATTGTATCAAATGGGCAAGGTTAGAATTTGAAAAAAAATTCAACCATGATATCAAGCAGTTACTGTTCAACTTCCCCAAGGAT
GCCAAGACTTCCAACGGTGAACCATTTTGGTCCGGTGCTAAGCGTGCTCCAACACCATTAGAGTTTGATATTTACAATAACGATCACTTCCATTTTGTCG
TAGCAGGTGCTAGTTTACGCGCTTATAATTATGGTATTAAAAGTGATGACTCTAATTCTAAACCCAACGTGGACGAGTATAAGTCCGTCATCGATCATAT
GATAATTCCAGAATTTACTCCAAATGCCAATTTGAAAATTCAAGTTAATGACGATGATCCGGATCCAAATGCCAACGCGGCTAATGGCTCAGATGAAATT
GATCAGCTGGTATCATCCCTACCAGATCCTTCTACTTTGGCTGGCTTTAAACTGGAGCCTGTAGATTTCGAGAAAGACGATGACACTAATCATCATATTG
AATTTATAACGGCTTGTTCTAACTGTAGAGCTCAAAACTACTTCATCGAAACTGCTGATCGCCAAAAGACCAAATTCATAGCTGGCCGTATTATTCCTGC
CATCGCAACAACCACTTCTTTGGTTACTGGTTTGGTCAATTTGGAATTATATAAACTGATTGACAACAAGACAGATATTGAGCAATATAAGAATGGCTTT
GTTAATTTAGCTTTGCCATTTTTCGGTTTTTCGGAACCAATTGCTTCACCAAAGGGAGAATATAACAACAAGAAATATGATAAAATTTGGGATAGATTTG
ATATCAAGGGAGATATTAAATTAAGTGATTTAATTGAACATTTTGAAAAAGATGAAGGGTTAGAAATAACAATGCTTTCTTATGGCGTTTCTCTTCTTTA
TGCCTCATTTTTCCCTCCAAAGAAATTGAAAGAAAGACTAAATCTACCAATAACTCAATTGGTTAAATTAGTGACTAAGAAAGATATTCCGGCACATGTA
TCTACAATGATTCTCGAAATTTGCGCAGATGACAAGGAAGGAGAGGATGTTGAAGTTCCTTTCATTACCATTCATCTATGAGCGGCCGC
Vector Type
Baculovirus
Antibiotic
Amp; Kan
Comments
Please can you make a vector for baculovirus expression of the budding yeast E1 for ubiquitin, which is called Uba1, with a 6His-TEV tag at the amino terminus? Then pass to Axel for expression. Thanks very much.
100042 R 1000 1001 1047 19007
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