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Plasmid
pcDNA5D FRT/TO BirA R118G-USP11 C318A-HA
Parent Plasmid
pcDNA5D FRT/TO BirA R118G MCS HA
Expressed
BirA R118G-USP11 C318A-HA
DU Number
DU24801
Genbank
NM_004651
Tag
HA
BirA
Species
Human
Sequence of Insert
View Sequence
GCGGCCGCTATGGCAGTAGCCCCGCGACTGTTTGGGGGGCTCTGCTTCCG
TTTCCGGGACCAGAATCCGGAAGTGGCTGTTGAGGGGCGTCTTCCAATCT
CGCACAGCTGCGTTGGCTGTAGAAGAGAACGGACGGCGATGGCGACGGTC
GCAGCAAATCCAGCTGCTGCTGCGGCGGCTGTGGCGGCGGCAGCGGCGGT
GACTGAGGATAGAGAGCCACAGCACGAGGAGCTGCCAGGCCTGGACAGCC
AGTGGCGCCAGATAGAAAACGGCGAGAGTGGGCGAGAACGTCCACTGCGG
GCCGGCGAAAGCTGGTTCCTTGTGGAGAAGCACTGGTATAAGCAGTGGGA
GGCATACGTGCAGGGAGGGGACCAGGACTCCAGCACCTTCCCTGGCTGCA
TCAACAATGCCACACTCTTTCAAGATGAGATAAACTGGCGCCTCAAGGAG
GGACTGGTGGAAGGCGAGGATTATGTGCTGCTCCCAGCAGCTGCTTGGCA
TTACCTGGTCAGCTGGTATGGTCTAGAGCATGGCCAGCCACCCATTGAAC
GCAAGGTCATAGAGCTGCCCAACATCCAGAAGGTCGAAGTGTACCCAGTA
GAACTGCTGCTTGTCCGGCACAATGATTTGGGCAAATCTCACACTGTTCA
GTTCAGCCATACCGATTCTATTGGCCTAGTATTGCGCACAGCTCGGGAGC
GGTTTCTGGTGGAGCCCCAGGAAGACACTCGGCTTTGGGCCAAGAACTCA
GAAGGCTCTTTGGATAGGTTGTATGACACACACATCACGGTTCTCGATGC
GGCCCTTGAGACTGGGCAGTTGATCATCATGGAGACCCGCAAGAAAGATG
GCACTTGGCCCAGCGCACAGCTGCATGTCATGAACAACAACATGTCGGAA
GAGGATGAGGACTTCAAGGGTCAGCCAGGCATCTGTGGCCTCACCAATCT
GGGCAACACGgcCTTCATGAACTCGGCCCTGCAGTGCCTCAGCAATGTGC
CACAGCTCACCGAGTACTTCCTCAACAACTGCTACCTGGAGGAGCTCAAC
TTCCGCAACCCACTGGGCATGAAGGGTGAGATCGCAGAGGCCTATGCAGA
CCTGGTGAAGCAGGCGTGGTCTGGCCACCACCGCTCCATTGTGCCACATG
TGTTCAAGAACAAGGTTGGCCATTTTGCATCCCAATTTCTGGGCTACCAG
CAGCATGACTCTCAGGAGCTGCTGTCATTCCTCCTGGACGGGCTGCATGA
GGACCTTAATCGGGTGAAGAAGAAGGAGTATGTGGAGCTGTGCGATGCTG
CTGGGCGACCGGATCAGGAGGTGGCACAGGAGGCATGGCAAAACCACAAA
CGGCGGAACGATTCTGTGATCGTGGACACTTTCCACGGCCTCTTCAAGTC
CACGCTGGTGTGCCCCGATTGTGGCAATGTATCTGTGACCTTCGACCCCT
TCTGCTACCTCAGTGTTCCACTGCCTATCAGCCACAAGAGGGTCTTGGAG
GTCTTCTTTATCCCCATGGATCCGCGCCGCAAGCCAGAGCAGCACCGGCT
CGTGGTCCCCAAGAAAGGCAAGATCTCGGATCTATGTGTGGCTCTGTCCA
AACACACGGGCATCTCGCCAGAGAGGATGATGGTGGCTGATGTCTTCAGT
CACCGCTTCTATAAGCTCTATCAGCTAGAGGAGCCTCTGAGCAGCATCTT
GGACCGTGATGATATCTTCGTCTATGAGGTGTCAGGTCGCATTGAGGCCA
TTGAGGGCTCAAGAGAGGACATCGTGGTTCCTGTCTACCTGCGGGAGCGC
ACCCCTGCCCGTGACTACAACAACTCCTACTACGGCCTGATGCTTTTTGG
ACACCCCCTCCTGGTATCAGTGCCCCGGGACCGCTTCACCTGGGAGGGCC
TGTATAACGTCCTGATGTACCGGCTCTCACGCTACGTGACCAAACCCAAC
TCAGATGATGAGGACGATGGGGATGAGAAAGAAGATGACGAGGAGGATAA
AGATGACGTCCCTGGGCCCTCAACTGGGGGCAGCCTCCGAGACCCTGAGC
CAGAGCAGGCTGGGCCCAGCTCTGGAGTCACGAACAGGTGCCCGTTCCTC
CTGGACAATTGCCTTGGCACATCTCAGTGGCCCCCAAGGCGACGACGCAA
GCAGCTGTTCACCCTGCAGACGGTGAACTCCAATGGGACCAGCGACCGCA
CAACCTCCCCTGAAGAAGTCCATGCCCAGCCGTACATTGCTATCGACTGG
GAGCCAGAGATGAAGAAGCGTTACTATGACGAGGTAGAGGCTGAGGGCTA
CGTGAAGCATGACTGCGTCGGGTACGTGATGAAGAAGGCTCCCGTGCGGC
TGCAGGAGTGCATTGAGCTCTTCACCACTGTGGAGACCCTGGAGAAGGAA
AACCCCTGGTACTGCCCTTCCTGCAAGCAGCACCAGCTGGCAACCAAGAA
GCTGGACCTGTGGATGCTGCCGGAGATTCTCATCATCCACCTGAAACGCT
TTTCCTACACCAAGTTCTCCCGAGAGAAGCTGGACACCCTCGTGGAGTTT
CCTATCCGGGACCTGGACTTCTCTGAGTTTGTCATCCAGCCACAGAATGA
GTCGAATCCGGAGCTGTACAAATATGACCTCATCGCGGTTTCCAACCATT
ATGGGGGCATGCGTGATGGACACTACACAACATTTGCCTGCAACAAGGAC
AGCGGCCAGTGGCACTACTTTGATGACAACAGCGTCTCCCCTGTCAATGA
GAATCAGATCGAGTCCAAGGCAGCCTATGTCCTCTTCTACCAACGCCAGG
ACGTGGCGCGACGCCTGCTGTCCCCGGCCGGCTCATCTGGCGCCCCAGCC
TCCCCTGCCTGCAGCTCCCCACCCAGCTCTGAGTTCATGGATGTTAATGC
GGCCGC
TTTCCGGGACCAGAATCCGGAAGTGGCTGTTGAGGGGCGTCTTCCAATCT
CGCACAGCTGCGTTGGCTGTAGAAGAGAACGGACGGCGATGGCGACGGTC
GCAGCAAATCCAGCTGCTGCTGCGGCGGCTGTGGCGGCGGCAGCGGCGGT
GACTGAGGATAGAGAGCCACAGCACGAGGAGCTGCCAGGCCTGGACAGCC
AGTGGCGCCAGATAGAAAACGGCGAGAGTGGGCGAGAACGTCCACTGCGG
GCCGGCGAAAGCTGGTTCCTTGTGGAGAAGCACTGGTATAAGCAGTGGGA
GGCATACGTGCAGGGAGGGGACCAGGACTCCAGCACCTTCCCTGGCTGCA
TCAACAATGCCACACTCTTTCAAGATGAGATAAACTGGCGCCTCAAGGAG
GGACTGGTGGAAGGCGAGGATTATGTGCTGCTCCCAGCAGCTGCTTGGCA
TTACCTGGTCAGCTGGTATGGTCTAGAGCATGGCCAGCCACCCATTGAAC
GCAAGGTCATAGAGCTGCCCAACATCCAGAAGGTCGAAGTGTACCCAGTA
GAACTGCTGCTTGTCCGGCACAATGATTTGGGCAAATCTCACACTGTTCA
GTTCAGCCATACCGATTCTATTGGCCTAGTATTGCGCACAGCTCGGGAGC
GGTTTCTGGTGGAGCCCCAGGAAGACACTCGGCTTTGGGCCAAGAACTCA
GAAGGCTCTTTGGATAGGTTGTATGACACACACATCACGGTTCTCGATGC
GGCCCTTGAGACTGGGCAGTTGATCATCATGGAGACCCGCAAGAAAGATG
GCACTTGGCCCAGCGCACAGCTGCATGTCATGAACAACAACATGTCGGAA
GAGGATGAGGACTTCAAGGGTCAGCCAGGCATCTGTGGCCTCACCAATCT
GGGCAACACGgcCTTCATGAACTCGGCCCTGCAGTGCCTCAGCAATGTGC
CACAGCTCACCGAGTACTTCCTCAACAACTGCTACCTGGAGGAGCTCAAC
TTCCGCAACCCACTGGGCATGAAGGGTGAGATCGCAGAGGCCTATGCAGA
CCTGGTGAAGCAGGCGTGGTCTGGCCACCACCGCTCCATTGTGCCACATG
TGTTCAAGAACAAGGTTGGCCATTTTGCATCCCAATTTCTGGGCTACCAG
CAGCATGACTCTCAGGAGCTGCTGTCATTCCTCCTGGACGGGCTGCATGA
GGACCTTAATCGGGTGAAGAAGAAGGAGTATGTGGAGCTGTGCGATGCTG
CTGGGCGACCGGATCAGGAGGTGGCACAGGAGGCATGGCAAAACCACAAA
CGGCGGAACGATTCTGTGATCGTGGACACTTTCCACGGCCTCTTCAAGTC
CACGCTGGTGTGCCCCGATTGTGGCAATGTATCTGTGACCTTCGACCCCT
TCTGCTACCTCAGTGTTCCACTGCCTATCAGCCACAAGAGGGTCTTGGAG
GTCTTCTTTATCCCCATGGATCCGCGCCGCAAGCCAGAGCAGCACCGGCT
CGTGGTCCCCAAGAAAGGCAAGATCTCGGATCTATGTGTGGCTCTGTCCA
AACACACGGGCATCTCGCCAGAGAGGATGATGGTGGCTGATGTCTTCAGT
CACCGCTTCTATAAGCTCTATCAGCTAGAGGAGCCTCTGAGCAGCATCTT
GGACCGTGATGATATCTTCGTCTATGAGGTGTCAGGTCGCATTGAGGCCA
TTGAGGGCTCAAGAGAGGACATCGTGGTTCCTGTCTACCTGCGGGAGCGC
ACCCCTGCCCGTGACTACAACAACTCCTACTACGGCCTGATGCTTTTTGG
ACACCCCCTCCTGGTATCAGTGCCCCGGGACCGCTTCACCTGGGAGGGCC
TGTATAACGTCCTGATGTACCGGCTCTCACGCTACGTGACCAAACCCAAC
TCAGATGATGAGGACGATGGGGATGAGAAAGAAGATGACGAGGAGGATAA
AGATGACGTCCCTGGGCCCTCAACTGGGGGCAGCCTCCGAGACCCTGAGC
CAGAGCAGGCTGGGCCCAGCTCTGGAGTCACGAACAGGTGCCCGTTCCTC
CTGGACAATTGCCTTGGCACATCTCAGTGGCCCCCAAGGCGACGACGCAA
GCAGCTGTTCACCCTGCAGACGGTGAACTCCAATGGGACCAGCGACCGCA
CAACCTCCCCTGAAGAAGTCCATGCCCAGCCGTACATTGCTATCGACTGG
GAGCCAGAGATGAAGAAGCGTTACTATGACGAGGTAGAGGCTGAGGGCTA
CGTGAAGCATGACTGCGTCGGGTACGTGATGAAGAAGGCTCCCGTGCGGC
TGCAGGAGTGCATTGAGCTCTTCACCACTGTGGAGACCCTGGAGAAGGAA
AACCCCTGGTACTGCCCTTCCTGCAAGCAGCACCAGCTGGCAACCAAGAA
GCTGGACCTGTGGATGCTGCCGGAGATTCTCATCATCCACCTGAAACGCT
TTTCCTACACCAAGTTCTCCCGAGAGAAGCTGGACACCCTCGTGGAGTTT
CCTATCCGGGACCTGGACTTCTCTGAGTTTGTCATCCAGCCACAGAATGA
GTCGAATCCGGAGCTGTACAAATATGACCTCATCGCGGTTTCCAACCATT
ATGGGGGCATGCGTGATGGACACTACACAACATTTGCCTGCAACAAGGAC
AGCGGCCAGTGGCACTACTTTGATGACAACAGCGTCTCCCCTGTCAATGA
GAATCAGATCGAGTCCAAGGCAGCCTATGTCCTCTTCTACCAACGCCAGG
ACGTGGCGCGACGCCTGCTGTCCCCGGCCGGCTCATCTGGCGCCCCAGCC
TCCCCTGCCTGCAGCTCCCCACCCAGCTCTGAGTTCATGGATGTTAATGC
GGCCGC
Amino Acid Sequence
View Sequence
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSPGEFASLEKLAAAMAVAPRLFGGLCFR
FRDQNPEVAVEGRLPISHSCVGCRRERTAMATVAANPAAAAAAVAAAAAV
TEDREPQHEELPGLDSQWRQIENGESGRERPLRAGESWFLVEKHWYKQWE
AYVQGGDQDSSTFPGCINNATLFQDEINWRLKEGLVEGEDYVLLPAAAWH
YLVSWYGLEHGQPPIERKVIELPNIQKVEVYPVELLLVRHNDLGKSHTVQ
FSHTDSIGLVLRTARERFLVEPQEDTRLWAKNSEGSLDRLYDTHITVLDA
ALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDEDFKGQPGICGLTNL
GNTAFMNSALQCLSNVPQLTEYFLNNCYLEELNFRNPLGMKGEIAEAYAD
LVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSFLLDGLHE
DLNRVKKKEYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTFHGLFKS
TLVCPDCGNVSVTFDPFCYLSVPLPISHKRVLEVFFIPMDPRRKPEQHRL
VVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLSSIL
DRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLMLFG
HPLLVSVPRDRFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDDEEDK
DDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRRRRK
QLFTLQTVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGY
VKHDCVGYVMKKAPVRLQECIELFTTVETLEKENPWYCPSCKQHQLATKK
LDLWMLPEILIIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEFVIQPQNE
SNPELYKYDLIAVSNHYGGMRDGHYTTFACNKDSGQWHYFDDNSVSPVNE
NQIESKAAYVLFYQRQDVARRLLSPAGSSGAPASPACSSPPSSEFMDVNA
AAYPYDVPDYA*
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSPGEFASLEKLAAAMAVAPRLFGGLCFR
FRDQNPEVAVEGRLPISHSCVGCRRERTAMATVAANPAAAAAAVAAAAAV
TEDREPQHEELPGLDSQWRQIENGESGRERPLRAGESWFLVEKHWYKQWE
AYVQGGDQDSSTFPGCINNATLFQDEINWRLKEGLVEGEDYVLLPAAAWH
YLVSWYGLEHGQPPIERKVIELPNIQKVEVYPVELLLVRHNDLGKSHTVQ
FSHTDSIGLVLRTARERFLVEPQEDTRLWAKNSEGSLDRLYDTHITVLDA
ALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDEDFKGQPGICGLTNL
GNTAFMNSALQCLSNVPQLTEYFLNNCYLEELNFRNPLGMKGEIAEAYAD
LVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSFLLDGLHE
DLNRVKKKEYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTFHGLFKS
TLVCPDCGNVSVTFDPFCYLSVPLPISHKRVLEVFFIPMDPRRKPEQHRL
VVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLSSIL
DRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLMLFG
HPLLVSVPRDRFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDDEEDK
DDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRRRRK
QLFTLQTVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGY
VKHDCVGYVMKKAPVRLQECIELFTTVETLEKENPWYCPSCKQHQLATKK
LDLWMLPEILIIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEFVIQPQNE
SNPELYKYDLIAVSNHYGGMRDGHYTTFACNKDSGQWHYFDDNSVSPVNE
NQIESKAAYVLFYQRQDVARRLLSPAGSSGAPASPACSSPPSSEFMDVNA
AAYPYDVPDYA*
Vector Type
Mammalian
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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