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Plasmid
pSc-B-ubiquitin KallR
Parent Plasmid
pSC-B
DU Number
DU24507
Genbank
NM_020640
Species
Human
Sequence of Insert
View Sequence
gcggccgctggcggctccggcggaggttccggaATGCAGATATTCGTGAG
GACCCTGACTGGTAGGACCATCACTCTCGAAGTGGAGCCGAGTGACACCA
TTGAGAATGTCAGGGCAAGGATCCAAGACAGGGAAGGCATCCCTCCTGAC
CAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACGCACCCT
GTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGGTCCTCCGTC
TCAGAgctgcgTGAgcggccgc
GACCCTGACTGGTAGGACCATCACTCTCGAAGTGGAGCCGAGTGACACCA
TTGAGAATGTCAGGGCAAGGATCCAAGACAGGGAAGGCATCCCTCCTGAC
CAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACGCACCCT
GTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGGTCCTCCGTC
TCAGAgctgcgTGAgcggccgc
Amino Acid Sequence
View Sequence
AAAGGSGGGSGMQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPD
QQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRAA*
QQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRAA*
Vector Type
Other
Antibiotic
Amp; Kan
Comments
Not1 ends with N-ter GGSGGGSG linker, plus stop. contains G75/76A substitutions at the C-teminus and a silent change @ nt9 (Ile3) to KO BglII site.
100042 R 1000 1001 1047 19007
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