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Ubiquitin

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Plasmid
pET24a 6His Ubiquitin
Parent Plasmid
pET24a original
Expressed
6His-Ubiquitin
DU Number
DU49503
Genbank
P62988*
Tag
6His
Species
Human
Sequence of Insert
ATGCATCACCATCACCATCACGGCGGTATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC


Amino Acid Sequence
MHHHHHHGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQ
RLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG*
Vector Type
Bacterial
Antibiotic
Kan
Comments
Bacterial expression construct for 6His-tagged ubiquitin (Arg73 codon AGA mutated to more common CGA)

Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389).



100042 R 1000 1001 1047 19007
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