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Synonyms
6030441H18Rik; Mtmr15
Plasmid
pBabe.puro FLAG.DU mouse FAN1 K975A
Parent Plasmid
pBabeD puro FLAG1
Expressed
FLAG mouse FAN1 K975A
DU Number
DU49099
Genbank
NM_177893.3 GI:257467663 with silent change a414g
Tag
FLAG
Species
Mouse
Sequence of Insert
View Sequence
GGATCCATGCCGTCACAAAGGAAATCACCTGATCAAAAAAGACCCCGTAGAAGCTTATCTACCAGCAAAACTGCAAAAAGCCAGTGTCATTCGATTACTTCGTATTTTAACAGTGCACCACCTGCTAAACTTGCATGTTCAACTTGTCATAAAATGGTGCCCAGGTATGACCTCATTCGGCACCTTGATGAGTCTTGTGCTAACAACGGTGTTGGTGATGACGTTCAAGTAGAGCCTGCTCAGGCTGGATTAATGAGTCCAACTGTGCCTACATCAGATTTACCCAGTGGTCCTTTAGAGAATGTGACACCTCAGAAATTATCACCACCAAAGAGAAGTTTAATTTCTGTCCAATGTGGTTCAAAACTGGGCATACAACAGCAGACCAGTCCCTACTTTAAAGATGCCTTAGTGTCCAAGGATCAAAATGAGCTTCCAAATCAGAGTGTAGAAATTATGCCTTTGGGAAGTCTGACATCGAAATTGTCCAGACGATACTTAAACGCTAAAAAATCACTCGCTAAGAATGAGGGACTAGCCAGTCAGTGCCCACAGACTTCTCCGTCCACACCTGGCACCAGCCTGACTGATAACTGTCCAGAGATGGAAGACAAAGATGAGGTTTTGAACAGTTCCCAAAAGGAAAACATTTATTCATGTGCACCTCTAAAAGAAGAGAATGCTTCAGAACAGAAGGTAAAGAACAATAAAATAACGGGAGATGAAAGCCAGAAAGCTTCCTGCGGGGAGCCAGCCCTCACCCCTGCATCTGCAGAGCATGCTTCCATATTACTGTCCTCAGATTCAACTCTCGTCAGTAACACGAAATCCTCTCCGGGAGACACTCTTGTAAAGCAAGAAAGTGCCAGAAGGGCAGATGTAGGGCTTGCTGAGCCGCTCGAGGTGCGCAGTCACAAAGAAGTACAAATGACTTTCGATGCTGCGGCCAAAACGCTGGTGTCTGGGGAGGCAGAATCAAATGGTCCTACCGATGTTGACATGTCTGACATGACTACGTGGAGTAACAACCAAGAGCTTGTCAGGGAAGCTGGCAGTGTCTTACACTGCCCTCTGGAGCAGGGGTCCAGCTGCGGTGGCCCCAGTGAGACAGCTCAACTGGCACTGAGTCACCCGTACTACCTGCGGAGCTTCCTGGTGGTGCTGCAGGCCCTCCTTGGGAACGAAGAGGACATGAAGCTCTTCGATGAGCAGGAGAAGGCGATTATCACCAGGTTTTACCAGTTGTCAGCTAGTGGTCAAAAGTTGTATGTGAGGCTCTTTCAACGTAAATTAACCTGGATTAAAATGAGTAAACTGGAGTATGAAGAGATTGCCTCCGACTTAACCCCTGTGGTTGAAGAATTGAAGGACTCAGGCTTTCTACAGACAGAATCTGAGTTGCAAGAACTCTCTGATGTACTTGAACTCCTTTCTGCTCCTGAGCTGAAAGCCCTGGCCAAAACCTTCCACTTGGTGAGTCCCGGTGGGCAGAAGCAGCAGCTGGTAGACGCCTTTCACAAACTGGCCAAACAGCGCTCAGTCTGCACGTGGGGCAAGACTCAGCCTGGAATCCGAGCAGTGATTTTAAAAAGAGCCAAAGACTTGGCTGGCCGGTCGCTTCGAGTCTGTAAAGGCCCTAGGGCTGTGTTTGCCCGAATCTTGCTTCTGTTCTCATTGACTGATTCCATGGAAGACGAAGAAGCTGCTTGTGGGGGTCAAGGTCAGCTGTCTACTGTGCTATTGGTCAATCTGGGCCGAATGGAGTTTCCTCAGTACACCATCTGCCGGAAGACCCAGATCTTCAGGGACAGAGAGGACCTCATCAGATACGCAGCAGCCGCACACATGCTGAGTGACATCTCAGCTGCCATGGCCAGTGGGAACTGGGAGGACGCTAAGGAGCTTGCTCGGAGTGCAAAAAGGGACTGGGAACAACTGAAAAGCCACCCTTCCCTGAGGTACCACGAGGCCTTGCCGCCCTTCCTGCGCTGCTTTACCGTCGGGTGGATCTACACAAGGATTTCCTCTCGGGCTGTTGAAGTCCTAGAGAGGCTTCACATGTACGAGGAAGCTGTCAAGGAACTTGAAAACCTCTTGTCTCAGAAAATCTATTGTCCTGACAGCAGAGGCCGCTGGTGGGATCGCCTGGCTCTTAACCTGCACCAGCACCTGAAGCGTCTGGAAGAGGCAATTAGGTGCATCAGGGAAGGCCTGGCAGACCCACACGTGAGGACAGGACACCGACTTTCTCTTTATCAGCGAGCCGTGCGCCTGCGAGAGTCTCCGAGCTGCAGGAAGTACAAGCACCTCTTCAGCCGGCTGCCCGAGGTTGCCGTGGGAGACGTCAAGCATGTGACCATCACGGGCAGGCTGTGCCCACAGCATGGCATGGGCAAGTCTGTGTTTGTCATGGAGAGTGGAGACGGCGCCAACCCCACCACAGTCCTGTGTTCTGTGGAGGAGCTGGCCCTGGGTTATTACAGGCAGAGTGGCTTTGACCAAGGGATTCATGGAGAAGGGTCCACCTTCAGCACCCTGTGTGGCCTCCTGCTGTGGGATATCATCTTCATGGATGGAATACCAGACGTCTTCAGAAATGCCTACCAGGCATCCCCACTGGATTTGCTCACGGACAGCTTCTTCGCAAGCAGGGAGCAGGCCCTGGAAGCCAGGCTGCAGCTGATCCACAGCGCCCCTGCTGAGAGCCTGCGGGCCTGGGTGGGCGAAGCATGGCAGGCCCAGCAAGGCAGAGTGGCCTCTCTGGTCAGCTGGGACCGCTTCACCTCCCTACAGCAAGCTCAGGATCTTGTCTCCTGCCTCGGGGGTCCTGTCCTCAGTGGTGTGTGCAGGCGCCTGGCTGCTGACTTTCGGCACTGCCGAGGGGGCCTCCCAGACTTGGTGGTGTGGAATTCTCAGAGCCACCATTGCGCGCTGGTGGAGGTGAAAGGCCCCAGTGATCGACTTTCATGTAAGCAGATGATCTGGCTGTACGAACTCCAAAAGCTGGGGGCTGATGTAGAAGTGTGCCATGTGGTTGCAGTTGGTGCTAAGAGCAAAGGTCTTGGCTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MDYKDDDDKGSMPSQRKSPDQKRPRRSLSTSKTAKSQCHSITSYFNSAPP
AKLACSTCHKMVPRYDLIRHLDESCANNGVGDDVQVEPAQAGLMSPTVPT
SDLPSGPLENVTPQKLSPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKD
QNELPNQSVEIMPLGSLTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTP
GTSLTDNCPEMEDKDEVLNSSQKENIYSCAPLKEENASEQKVKNNKITGD
ESQKASCGEPALTPASAEHASILLSSDSTLVSNTKSSPGDTLVKQESARR
ADVGLAEPLEVRSHKEVQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWS
NNQELVREAGSVLHCPLEQGSSCGGPSETAQLALSHPYYLRSFLVVLQAL
LGNEEDMKLFDEQEKAIITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEY
EEIASDLTPVVEELKDSGFLQTESELQELSDVLELLSAPELKALAKTFHL
VSPGGQKQQLVDAFHKLAKQRSVCTWGKTQPGIRAVILKRAKDLAGRSLR
VCKGPRAVFARILLLFSLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQ
YTICRKTQIFRDREDLIRYAAAAHMLSDISAAMASGNWEDAKELARSAKR
DWEQLKSHPSLRYHEALPPFLRCFTVGWIYTRISSRAVEVLERLHMYEEA
VKELENLLSQKIYCPDSRGRWWDRLALNLHQHLKRLEEAIRCIREGLADP
HVRTGHRLSLYQRAVRLRESPSCRKYKHLFSRLPEVAVGDVKHVTITGRL
CPQHGMGKSVFVMESGDGANPTTVLCSVEELALGYYRQSGFDQGIHGEGS
TFSTLCGLLLWDIIFMDGIPDVFRNAYQASPLDLLTDSFFASREQALEAR
LQLIHSAPAESLRAWVGEAWQAQQGRVASLVSWDRFTSLQQAQDLVSCLG
GPVLSGVCRRLAADFRHCRGGLPDLVVWNSQSHHCALVEVKGPSDRLSCK
QMIWLYELQKLGADVEVCHVVAVGAKSKGLG*
AKLACSTCHKMVPRYDLIRHLDESCANNGVGDDVQVEPAQAGLMSPTVPT
SDLPSGPLENVTPQKLSPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKD
QNELPNQSVEIMPLGSLTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTP
GTSLTDNCPEMEDKDEVLNSSQKENIYSCAPLKEENASEQKVKNNKITGD
ESQKASCGEPALTPASAEHASILLSSDSTLVSNTKSSPGDTLVKQESARR
ADVGLAEPLEVRSHKEVQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWS
NNQELVREAGSVLHCPLEQGSSCGGPSETAQLALSHPYYLRSFLVVLQAL
LGNEEDMKLFDEQEKAIITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEY
EEIASDLTPVVEELKDSGFLQTESELQELSDVLELLSAPELKALAKTFHL
VSPGGQKQQLVDAFHKLAKQRSVCTWGKTQPGIRAVILKRAKDLAGRSLR
VCKGPRAVFARILLLFSLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQ
YTICRKTQIFRDREDLIRYAAAAHMLSDISAAMASGNWEDAKELARSAKR
DWEQLKSHPSLRYHEALPPFLRCFTVGWIYTRISSRAVEVLERLHMYEEA
VKELENLLSQKIYCPDSRGRWWDRLALNLHQHLKRLEEAIRCIREGLADP
HVRTGHRLSLYQRAVRLRESPSCRKYKHLFSRLPEVAVGDVKHVTITGRL
CPQHGMGKSVFVMESGDGANPTTVLCSVEELALGYYRQSGFDQGIHGEGS
TFSTLCGLLLWDIIFMDGIPDVFRNAYQASPLDLLTDSFFASREQALEAR
LQLIHSAPAESLRAWVGEAWQAQQGRVASLVSWDRFTSLQQAQDLVSCLG
GPVLSGVCRRLAADFRHCRGGLPDLVVWNSQSHHCALVEVKGPSDRLSCK
QMIWLYELQKLGADVEVCHVVAVGAKSKGLG*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Propagate at 30 degrees
100042 R 1000 1001 1047 19007
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