ubiquitin

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Plasmid
pGEX6p-1 Ubiquitin G75A G76A Flash
Parent Plasmid
pGEX6P1
Expressed
GST-Precission-ubiquitin-FLASH
DU Number
DU25285
Tag
GST
FLASH
Species
Human
Sequence of Insert
GGATCCGGCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCTGCTTGATAAGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGAMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRAAGGSGGCCPGCCGG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Ecoli optimised Flash tag on c terminal
Each ubiquitin differs from ref sequence in having C-terminal G75/76 AA instead of GG.


100042 R 1000 1001 1047 19007
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