This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Plasmid
pGEX6P HECTD2 F373-E776(end) (With mutations L519F and D540G)
Parent Plasmid
pGEX6P1
Expressed
GST-C3-HECTD2 F373-E776(end) (With mutations L519F and D540G)
DU Number
DU45770
Genbank
NM_182765.3 GI:190341083 (With EST-derived mutations L519F and D540G)
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCTTCGTTATTTCTGTAGCTGCAAAAAAAATCATTATTCAGAGAGACTCAGAGCAACAGATGATAAACATCGCAAGGCAAAGTCTGGTGGATAAAGTATCTCGAAGACAGAGACCTGATATGAATATATTATTTCTAAATATGAAAGTAAGACGGACACATCTGGTTAGCGATTCACTTGATGAGCTAACCCGGAAAAGAGCCGATTTGAAAAAGAAGTTAAAAGTTACATTTGTAGGGGAAGCTGGTTTGGATATGGGTGGTTTGACTAAAGAATGGTTCCTTCTTCTAATTCGCCAAATTTTTCATCCAGATTATGGCATGTTTACATATCACAAGGATTCACACTGCCATTGGTTTAGCAGCTTTAAATGTGATAACTATTCTGAATTCCGATTGGTTGGAATTCTTATGGGACTAGCTGTTTATAACAGCATCACCTTCGATATTCGTTTCCCTCCCTGCTGTTACAAGAAATTATTGAGCCCTCCCATCATTCCTAGTGGTCAAAATATACCAGTAGGCATCTGCAATGTTACCGTGGACGACTTATGTCAAATTATGCCTGAGTTGGCCCATGGATTAAGTGAACTCTTATCACATGAAGGCAATGTTGAAGAAGATTTCTATTCAACATTTCAGGTTTTTCAAGAAGAATTTGGAATAATCAAGTCCTATAATTTAAAGCCCGGTGGTGATAAAATTTCAGTTACCAATCAAAATAGAAAAGAATATGTACAGCTTTATACTGACTTCCTTCTGAACAAATCCATCTATAAGCAGTTTGCTGCATTTTATTATGGATTTCATAGTGTGTGTGCTTCAAATGCCCTAATGCTGCTTCGTCCAGAAGAGGTTGAAATTTTGGTCTGTGGAAGTCCTGACCTGGATATGCATGCTCTGCAGAGAAGTACTCAGTATGATGGCTATGCAAAAACGGACTTAACTATAAAATACTTTTGGGATGTTGTGCTTGGATTTCCTCTTGATCTTCAAAAGAAGTTGCTACATTTTACTACAGGGAGTGACAGAGTACCTGTAGGAGGGATGGCTGATTTGAACTTTAAAATCTCAAAGAATGAAACTTCTACTAACTGCTTACCTGTGGCCCATACCTGCTTCAATCAACTTTGCCTTCCCCCCTACAAGAGCAAAAAGGATCTGAAACAGAAATTGATCATTGGAATTTCAAATTCAGAAGGTTTTGGACTTGAGTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSFVISVAAKKIIIQRDSEQQ
MINIARQSLVDKVSRRQRPDMNILFLNMKVRRTHLVSDSLDELTRKRADL
KKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWF
SSFKCDNYSEFRLVGILMGLAVYNSITFDIRFPPCCYKKLLSPPIIPSGQ
NIPVGICNVTVDDLCQIMPELAHGLSELLSHEGNVEEDFYSTFQVFQEEF
GIIKSYNLKPGGDKISVTNQNRKEYVQLYTDFLLNKSIYKQFAAFYYGFH
SVCASNALMLLRPEEVEILVCGSPDLDMHALQRSTQYDGYAKTDLTIKYF
WDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNCLPVA
HTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSFVISVAAKKIIIQRDSEQQ
MINIARQSLVDKVSRRQRPDMNILFLNMKVRRTHLVSDSLDELTRKRADL
KKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWF
SSFKCDNYSEFRLVGILMGLAVYNSITFDIRFPPCCYKKLLSPPIIPSGQ
NIPVGICNVTVDDLCQIMPELAHGLSELLSHEGNVEEDFYSTFQVFQEEF
GIIKSYNLKPGGDKISVTNQNRKEYVQLYTDFLLNKSIYKQFAAFYYGFH
SVCASNALMLLRPEEVEILVCGSPDLDMHALQRSTQYDGYAKTDLTIKYF
WDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNCLPVA
HTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet