RNF220

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Plasmid
pEFIRES-P-eYFP C1-RNF220 g420a, t426c, c432t
Parent Plasmid
pEFIRES puro eYFPC1
Expressed
eYFP-RNF220 g420a, t426c, c432t
DU Number
DU20625
Genbank
NM_018150.2
Tag
YFP
Species
Human
Sequence of Insert
GGATCCATGGACTTACACCGGGCAGCCTTCAAGATGGAGAACTCATCCTA
CCTTCCCAACCCTCTGGCATCCCCAGCACTGATGGTCCTGGCATCCACGG
CTGAGGCCAGCCGTGATGCTTCCATCCCTTGTCAGCAGCCACGACCCTTT
GGTGTACCTGTCTCAGTTGACAAGGACGTGCATATTCCTTTCACCAACGG
TTCCTATACCTTTGCCTCTATGTACCATCGGCAAGGTGGGGTGCCAGGCA
CTTTTGCCAATCGTGATTTCCCCCCTTCTCTACTACACCTCCACCCTCAA
TTTGCTCCCCCAAATCTAGATTGCACCCCAATCAGTATGCTGAATCATAG
TGGTGTGGGGGCTTTCCGGCCCTTTGCCTCCACCGAGGACCGGGAGAGCT
ATCAGTCAGCCTTTACGCCGGCCAAACGACTCAAGAATTGCCATGACACA
GAGTCTCCCCACTTGCGCTTCTCAGATGCAGATGGCAAGGAATATGACTT
TGGGACACAGCTGCCATCTAGCTCCCCCGGTTCACTAAAGGTTGATGACA
CTGGGAAGAAGATTTTTGCTGTCTCTGGCCTCATTTCTGATCGGGAAGCC
TCATCTAGCCCAGAGGATCGGAATGACAGATGTAAGAAGAAAGCAGCGGC
ATTGTTCGACAGCCAGGCCCCAATTTGCCCCATCTGCCAGGTCCTGCTGA
GGCCCAGTGAGCTGCAGGAGCATATGGAGCAGGAACTGGAGCAGCTAGCC
CAACTGCCCTCGAGCAAGAATTCCCTTCTGAAGGATGCCATGGCTCCAGG
CACCCCAAAGTCCCTCCTGTTGTCTGCTTCCATCAAGAGGGAAGGAGAGT
CTCCAACGGCATCACCCCACTCATCTGCCACCGATGACCTCCACCATTCA
GACAGATACCAGACCTTTCTGCGAGTACGAGCCAACCGGCAGACCCGACT
GAATGCTCGGATTGGGAAAATGAAACGGAGGAAGCAAGATGAAGGGCAGA
GGGAAGGCTCCTGCATGGCTGAGGATGATGCTGTGGACATCGAGCATGAG
AACAACAACCGCTTTGAGGAGTATGAGTGGTGTGGACAGAAGCGGATACG
GGCCACCACTCTCCTGGAAGGTGGCTTCCGAGGCTCTGGCTTCATCATGT
GCAGCGGCAAAGAGAACCCGGACAGTGATGCTGACTTGGATGTGGATGGG
GATGACACTCTGGAGTATGGGAAGCCACAATACACAGAGGCTGATGTCAT
CCCCTGCACAGGCGAGGAGCCTGGTGAAGCCAAGGAGAGAGAGGCACTTC
GGGGCGCAGTCCTAAATGGCGGCCCTCCCAGCACGCGCATCACACCTGAG
TTCTCTAAATGGGCCAGTGATGAGATGCCATCCACCAGCAATGGTGAAAG
CAGCAAGCAGGAGGCCATGCAGAAGACCTGCAAGAACAGCGACATCGAGA
AAATCACCGAAGATTCAGCTGTGACCACGTTTGAGGCTCTGAAGGCTCGG
GTCAGAGAACTTGAACGGCAGCTATCTCGTGGGGACCGTTACAAATGCCT
CATCTGCATGGACTCGTACTCGATGCCCCTAACGTCCATCCAGTGTTGGC
ACGTGCACTGCGAGGAGTGCTGGCTGCGGACCCTGGGTGCCAAGAAGCTC
TGCCCTCAGTGCAACACGATCACAGCGCCCGGAGACCTGCGGAGGATCTA
CTTGTGAGCGGCCGC
Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSMDLHRA
AFKMENSSYLPNPLASPALMVLASTAEASRDASIPCQQPRPFGVPVSVDK
DVHIPFTNGSYTFASMYHRQGGVPGTFANRDFPPSLLHLHPQFAPPNLDC
TPISMLNHSGVGAFRPFASTEDRESYQSAFTPAKRLKNCHDTESPHLRFS
DADGKEYDFGTQLPSSSPGSLKVDDTGKKIFAVSGLISDREASSSPEDRN
DRCKKKAAALFDSQAPICPICQVLLRPSELQEHMEQELEQLAQLPSSKNS
LLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLR
VRANRQTRLNARIGKMKRRKQDEGQREGSCMAEDDAVDIEHENNNRFEEY
EWCGQKRIRATTLLEGGFRGSGFIMCSGKENPDSDADLDVDGDDTLEYGK
PQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDE
MPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQL
SRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTIT
APGDLRRIYL*


Vector Type
Mammalian
Antibiotic
Amp
Comments
Mammalian expression vector for eYFP-RNF220 g420a, t426c, c432t. EF1a promoter, IRES and puromycin selection cassette
Parental PLasmid 20522
Estimated MW of expressed protein : 90240 Daltons
100042 R 1000 1001 1047 19007
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