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Synonyms
-
Plasmid
pET15b-TEV-DiUbiquitin
Parent Plasmid
pET15b 6His TEV1
Expressed
6His-Ubiquitin dimer
DU Number
DU20503
Genbank
P62988*
Tag
6His
Species
Human
Sequence of Insert
View Sequence
GGATCCAAGATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCAC
TCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCC
AAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGG
AAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGA
GTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGCGGCATGCAGATCTTCG
TGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGAC
ACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCC
TGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCA
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTC
CGTCTCAGAGGTGGGTGATAAGGATCC
TCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCC
AAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGG
AAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGA
GTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGCGGCATGCAGATCTTCG
TGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGAC
ACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCC
TGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCA
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTC
CGTCTCAGAGGTGGGTGATAAGGATCC
Amino Acid Sequence
View Sequence
MGSSHHHHHHSSGENLYFQGHMLEGSKMQIFVKTLTGKTITLEVEPSDTI
ENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRL
RGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAG
KQLEDGRTLSDYNIQKESTLHLVLRLRGG**
ENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRL
RGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAG
KQLEDGRTLSDYNIQKESTLHLVLRLRGG**
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
*after subcloning, mutagenic oligos NW415/NW416 were used to correct frame
Bacterial expression construct to express wild type ubiquitin dimer with N-terminal 6HIS tag (contains TEV protease site). Also N-terminal lysine for tryptic cleavage
Estimated MW of expressed protein : 20163 Daltons