TRAFD1 | Ubiquitylation Resource

Plasmid

pET156P-1-TRAFD1

Parent Plasmid

pET15b 6His 3C

Expressed

6His-TRAFD1

DU Number

DU20137

Genbank

NM_006700.1

Tag

6His

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGCTGAATTTCTAGATGACCAGGAAACTCGACTGTGTGACAA
CTGCAAAAAAGAAATTCCTGTGTTTAACTTTACCATCCATGAGATCCACT
GTCAAAGGAACATTGGTATGTGTCCTACCTGTAAGGAACCATTTCCCAAA
TCTGACATGGAGACTCACATGGCTGCAGAACACTGTCAGGTGACCTGCAA
ATGTAACAAGAAGTTGGAGAAGAGGCTGTTAAAGAAGCATGAGGAGACTG
AGTGCCCTTTGCGGCTTGCTGTCTGCCAGCACTGTGATTTAGAACTTTCC
ATTCTCAAACTGAAGGAACATGAAGATTATTGTGGTGCCCGGACGGAACT
ATGTGGCAACTGTGGTCGCAATGTCCTTGTGAAAGATCTGAAGACTCACC
CTGAAGTTTGTGGGAGAGAGGGGGAGGAAAAGAGAAATGAGGTTGCCATA
CCTCCTAATGCATATGATGAATCTTGGGGTCAGGATGGAATCTGGATTGC
ATCCCAACTCCTCAGACAAATTGAGGCTCTGGACCCACCCATGAGGCTGC
CGCGAAGGCCCCTGAGAGCCTTTGAATCAGATGTTTTCCACAATAGAACT
ACCAACCAAAGGAACATTACAGCCCAGGTTTCAATTCAGAATAATCTGTT
TGAAGAACAAGAGAGGCAGGAAAGGAATAGAGGCCAACAGCCCCCCAAAG
AGGGTGGTGAAGAGAGTGCAAACTTGGACTTCATGTTGGCCCTAAGTCTG
CAAAATGAAGGCCAAGCCTCCAGTGTGGCAGAGCAGGACTTCTGGAGGGC
CGTATGTGAGGCCGACCAGTCTCATGGCGGTCCCAGGTCTCTCAGTGACA
TAAAGGGTGCAGCTGACGAGATCATGTTGCCTTGTGAATTTTGTGAGGAG
CTCTACCCAGAGGAACTGCTGATTGACCATCAGACAAGCTGTAACCCTTC
ACGTGCCTTACCTTCACTCAATACTGGCAGCTCTTCCCCCAGAGGGGTGG
AGGAACCTGATGTCATCTTCCAGAACTTCTTGCAACAGGCTGCAAGTAAC
CAGTTAGACTCTTTGATGGGCCTGAGCAATTCACACCCTGTGGAGGAGAG
CATCATTATCCCATGTGAATTCTGTGGGGTACAGCTGGAAGAGGAGGTGC
TGTTCCATCACCAGGACCAGTGTGACCAACGCCCAGCCACTGCAACCAAC
CATGTGACAGAGGGGATTCCTAGACTGGATTCCCAGCCTCAAGAGACCTC
ACCAGAGCTGCCCAGGAGGCGTGTCAGACACCAGGGAGACCTGTCTTCTG
GTTACCTGGATGATACTAAGCAGGAAACAGCTAATGGGCCCACCTCCTGT
CTGCCTCCCAGCCGACCCATTAACAATATGACAGCTACCTATAACCAGCT
ATCGAGATCAACATCAGGCCCCAGACCTGGGTGCCAGCCCAGCTCTCCTT
GTGTGCCGAAGCTCAGCAACTCAGACAGCCAGGACATCCAGGGGCGGAAT
CGAGACAGCCAGAATGGGGCCATAGCCCCTGGGCACGTTTCAGTGATTCG
CCCTCCTCAAAATCTCTACCCAGAAAACATTGTGCCCTCTTTCTCCCCTG
GGCCTTCAGGGAGATACGGAGCTAGTGGTAGGAGTGAAGGTGGCAGGAAT
TCCCGGGTCACCCCTGCAGCTGCCAACTACCGCAGCAGAACTGCAAAGGC
AAAGCCTTCCAAGCAACAGGGAGCTGGGGATGCAGAAGAGGAAGAGGAGG
AGTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAEFLDDQETRLCDNCKKEIPVFNFTI
HEIHCQRNIGMCPTCKEPFPKSDMETHMAAEHCQVTCKCNKKLEKRLLKK
HEETECPLRLAVCQHCDLELSILKLKEHEDYCGARTELCGNCGRNVLVKD
LKTHPEVCGREGEEKRNEVAIPPNAYDESWGQDGIWIASQLLRQIEALDP
PMRLPRRPLRAFESDVFHNRTTNQRNITAQVSIQNNLFEEQERQERNRGQ
QPPKEGGEESANLDFMLALSLQNEGQASSVAEQDFWRAVCEADQSHGGPR
SLSDIKGAADEIMLPCEFCEELYPEELLIDHQTSCNPSRALPSLNTGSSS
PRGVEEPDVIFQNFLQQAASNQLDSLMGLSNSHPVEESIIIPCEFCGVQL
EEEVLFHHQDQCDQRPATATNHVTEGIPRLDSQPQETSPELPRRRVRHQG
DLSSGYLDDTKQETANGPTSCLPPSRPINNMTATYNQLSRSTSGPRPGCQ
PSSPCVPKLSNSDSQDIQGRNRDSQNGAIAPGHVSVIRPPQNLYPENIVP
SFSPGPSGRYGASGRSEGGRNSRVTPAAANYRSRTAKAKPSKQQGAGDAE
EEEEE*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Bacterial expression construct to express TRAFD1 with N-terminal 6HIS tag (contains preScission protease site)

Estimated Mw of expressed protein : 67286 Daltons

100042 R 1000 1001 1047 19007