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Plasmid
pGEX-TEV-Ubiquitin (c220a mutant)
Parent Plasmid
pGEX TEV
Expressed
GST-Ubiquitin (c220a mutant)
DU Number
DU20776
Genbank
P62988*
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCGGTATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCAC
TCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCC
AAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGG
AAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGA
GTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGAATTC
TCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCC
AAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGG
AAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGA
GTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGAATTC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSGMQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
LRLRGG**
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSGMQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
LRLRGG**
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Bacterial expression vector with N-terminal GST tag and TEV protease cleavage site.
Estimated MW of expressed protein : 35301 Daltons
Parental: 20757