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Ubiquitin

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Plasmid
pET24-Ubiquitin c220a K63R mutant
Parent Plasmid
pET24a original
Expressed
untagged Ubiquitin c220a mutant K63R
DU Number
DU20029
Genbank
P62988*
Tag
untagged
Species
Human
Sequence of Insert
CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTG
GAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCT
GCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC

Amino Acid Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQRESTLHLVLRLRGG

Vector Type
Bacterial
Antibiotic
Kan
Comments
Bacterial expression construct for untagged ubiquitin K63R mutant.
Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389) and by K63R substitution conferred by primers MP1758/MP1759

Estimated MW of expressed protein : 8593 Daltons
100042 R 1000 1001 1047 19007
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