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Plasmid
pHis-TEV30a-YFP Venus-Ubiquitin
Parent Plasmid
pHIS TEV30a YFP
Expressed
YFP-Ubiquitin
DU Number
DU20857
Genbank
P62988
Tag
YFP
Sequence of Insert
View Sequence
GGATCCGGCTCCGGCTCCATGCAGATCTTCGTGAAGACCCTGACTGGTAA
GACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGG
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC
TTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACAT
CCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGAT
AAGAATTC
GACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGG
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC
TTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACAT
CCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGAT
AAGAATTC
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICT
TGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSGSGSMQIFV
KTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRT
LSDYNIQKESTLHLVLRLRGG**
TGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSGSGSMQIFV
KTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRT
LSDYNIQKESTLHLVLRLRGG**
Antibiotic
Amp
Comments
Bacterial YFP Venus ubiquitin. No 6His (YFP subcloned into Nco1 site of pHISTEV30a). Vector obtained from Hay group
100042 R 1000 1001 1047 19007
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