Ubiquitin

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Plasmid
pET28a-FLAG-Ubiquitin
Parent Plasmid
pET28a original
Expressed
FLAG-Ubiquitin
DU Number
DU20637
Genbank
P62988
Tag
FLAG
Species
Human
Sequence of Insert
CCATGGACTACAAGGACGACGATGACAAGATGCAAATCTTCGTGAAGACC
CTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGA
GAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGC
AGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCT
GACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAG
AGGTGGGTGATAACTCGAGAGATCC
Amino Acid Sequence
MDYKDDDDKMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQ
RLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG**
Vector Type
Bacterial
Antibiotic
Amp
Comments
*6HIS and T7 tags removed by subcloning Nco1 BglII into Nco1 BamH1 when DU35145 was created
N terminal FLAG tag for bacterial expression of wild type ubiquitin
100042 R 1000 1001 1047 19007
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