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Plasmid
pGEX-TEV-Ubiquitin (c220a mutant) duplicated TEV site
Parent Plasmid
pGEX TEV TEV
Expressed
GST-Ubiquitin (c220a mutant)
DU Number
DU20756
Genbank
P62988*
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCGGTATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCAC
TCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCC
AAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGG
AAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGA
GTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGAATTC
TCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCC
AAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGG
AAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGA
GTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGAATTC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQDHPPKSDENLYFQGGSGMQIFVKT
LTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLS
DYNIQKESTLHLVLRLRGG**
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQDHPPKSDENLYFQGGSGMQIFVKT
LTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLS
DYNIQKESTLHLVLRLRGG**
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
GST - ubiquitin (Arg73 codon AGA mutated to more common CGA) with 2 TEV cleavage sites
Estimated MW of expressed protein : 36873 Daltons
Parental: 20712