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Ubiquitin

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Plasmid
pcDNA5-FRT/TO-DAC-A-C3 Ubiquitin G75A G76A tetramer
Parent Plasmid
pcDNA5 FRT/TO DAC 3C
Expressed
DAC-A-C3 Ubiquitin G75A G76A tetramer
DU Number
DU20571
Genbank
P62988
Tag
DAC
Species
Human
Sequence of Insert
GGATCCGGCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCTGCTTGATAAGCGGCCGC

Amino Acid Sequence
MIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQA
MKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGIN
LQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADG
QYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSL
NVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLT
WGFRFFETVNPLEVLFQGPGSGAMQIFVKTLTGKTITLEVEPSDTIENVK
AKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRAAM
QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLE
DGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEPSDTIEN
VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRA
AMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQ
LEDGRTLSDYNIQKESTLHLVLRLRAA**

Vector Type
Mammalian
Antibiotic
Amp
Comments
Flp-In vector for mammalian expression of N-terminally DAC-A tagged G75A G76A tetraubiquitin (with initiating methionines)
Parental Plasmid DU30210
100042 R 1000 1001 1047 19007
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