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Ubiquitin

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Plasmid
pGEX6P-1-Ubiquitin monomer (Bam-Nae-Ubiquitin-Not)
Parent Plasmid
pGEX6P1
DU Number
DU20709
Genbank
P62988
Species
Human
Sequence of Insert

GGATCCGCCGGCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACC
ATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAA
GATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTG
CTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAG
AAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGCGGCTGATAAGC
GGCCGC

Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAGMQIFVKTLTGKTITLEV
EPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTL
HLVLRLRGG**
Vector Type
Bacterial
Antibiotic
Amp; Kan
Comments
GST-ubiquitin (wild type) monomer and intermediate in the assembly of wild type polyubiquitin chains

Estimated MW of expressed protein : 35517 Daltons
100042 R 1000 1001 1047 19007
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