Ubiquitin

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Plasmid
pSC-B-Ubiquitin G75A G76A trimer
Parent Plasmid
pSC-B
DU Number
DU20123
Genbank
P62988*
Species
Human
Sequence of Insert
GGCgccATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCT
CGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAG
ACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAA
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTC
CACCCTGCACCTGGTCCTCCGTCTCAGAgccgccATGCAGATCTTCGTGA
AGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACC
ATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGA
CCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCC
TGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGT
CTCAGAgccgccATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCAT
CACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGA
TCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCT
GGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAA
AGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAgccGGC
Amino Acid Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQ
LEDGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEPSDTI
ENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRL
RAAMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAG
KQLEDGRTLSDYNIQKESTLHLVLRLRA
Vector Type
Other
Antibiotic
Amp
Comments
Vector harbouring trimeric SfoI – NaeI ubiquitin unit (with flanking alanines and no STOP codon) for progressive assembly of polyubiquitin chains.
Differs from reference sequence: C-terminal single Ala instead of GG. N-terminal single Ala.
100042 R 1000 1001 1047 19007
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