Itch | Ubiquitylation Resource

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Plasmid

pCMV5 C-Myc-Itch C830A

Parent Plasmid

pCMV5

Expressed

cMyc-Itch C830A

DU Number

DU11354

Genbank

NM_031483

Tag

Myc

Species

Human

Sequence of Insert

View Sequence

ggtaccgccaccatggaacaaaaacttatttctgaagaagatctgtctgacagtggatcacaacttggttcaatgggtagcctcaccatgaaatcacagcttcagatcactgtcatctcagcaaaacttaaggaaaataagaagaattggtttggaccaagtccttacgtagaggtcacagtagatggacagtcaaagaagacagaaaaatgcaacaacacaaacagtcccaagtggaagcaaccccttacagttatcgttacccctgtgagtaaattacattttcgtgtgtggagtcaccagacactgaaatctgatgttttgttgggaactgctgcattagatatttatgaaacattaaagtcaaacaatatgaaacttgaagaagtagttgtgactttgcagcttggaggtgacaaagagccaacagagacaataggagacttgtcaatttgtcttgatgggctacagttagagtctgaagttgttaccaatggtgaaactacatgttcagaaagtgcttctcagaatgatgatggctccagatccaaggatgaaacaagagtgagcacaaatggatcagatgaccctgaagatgcaggagctggtgaa
aataggagagtcagtgggaataattctccatcactctcaaatggtggttt
taaaccttctagacctccaagaccttcacgaccaccaccacccaccccac
gtagaccagcatctgtcaatggttcaccatctgccacttctgaaagtgat
gggtctagtacaggctctctgccgccgacaaatacaaatacaaatacatc
tgaaggagcaacatctggattaataattcctcttactatatctggaggct
caggccctaggccattaaatcctgtaactcaagctcccttgccacctggt
tgggagcagagagtggaccagcacgggcgagtttactatgtagatcatgt
tgagaaaagaacaacatgggatagaccagaacctctacctcctggctggg
aacggcgggttgacaacatgggacgtatttattatgttgaccatttcaca
agaacaacaacgtggcagaggccaacactggaatccgtccggaactatga
acaatggcagctacagcgtagtcagcttcaaggagcaatgcagcagttta
accagagattcatttatgggaatcaagatttatttgctacatcacaaagt
aaagaatttgatcctcttggtccattgccacctggatgggagaagagaac
agacagcaatggcagagtatatttcgtcaaccacaacacacgaattacac
aatgggaagaccccagaagtcaaggtcaattaaatgaaaagcccttacct
gaaggttgggaaatgagattcacagtggatggaattccatattttgtgga
ccacaatagaagaactaccacctatatagatccccgcacaggaaaatctg
ccctagacaatggacctcagatagcctatgttcgggacttcaaagcaaag
gttcagtatttccggttctggtgtcagcaactggccatgccacagcacat
aaagattacagtgacaagaaaaacattgtttgaggattcctttcaacaga
taatgagcttcagtccccaagatctgcgaagacgtttgtgggtgattttt
ccaggagaagaaggtttagattatggaggtgtagcaagagaatggttctt
tcttttgtcacatgaagtgttgaacccaatgtattgcctgtttgaatatg
cagggaaggataactactgcttgcagataaaccccgcttcttacatcaat
ccagatcacctgaaatattttcgttttattggcagattcattgccatggc
tctgttccatgggaaattcatagacacgggtttttctttaccattctata
agcgtatcttgaacaaaccagttggactcaaggatttagaatctattgat
ccagaattttacaattctctcatctgggttaaggaaaacaatattgagga
atgtgatttggaaatgtacttctccgttgacaaagaaattctaggtgaaa
ttaagagtcatgatctgaaacctaatggtggcaatattcttgtaacagaa
gaaaataaagaggaatacatcagaatggtagctgagtggaggttgtctcg
aggtgttgaagaacagacacaagctttctttgaaggctttaatgaaattc
ttccccagcaatatttgcaatactttgatgcaaaggaattagaggtcctt
ttatgtggaatgcaagagattgatttgaatgactggcaaagacatgccat
ctaccgtcattatgcaaggaccagcaaacaaatcatgtggttttggcagt
ttgttaaagaaattgataatgagaagagaatgagacttctgcagtttgtt
actggaacctgccgattgccagtaggaggatttgctgatctcatggggag
caatggaccacagaaattctgcattgaaaaagttgggaaagaaaattggc
tacccagaagtcataccgcttttaatcgcctggacctgccaccatacaag
agctatgagcaactgaaggaaaagctgttgtttgccatagaagaaacaga
aggatttggacaagagtaaggatcc

Amino Acid Sequence

View Sequence

MEQKLISEEDLSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPS
PYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLK
SDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLD
GLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGE
NRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESD
GSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPG
WEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFT
RTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQS
KEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLP
EGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAK
VQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIF
PGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYIN
PDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESID
PEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTE
ENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVL
LCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFV
TGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTAFNRLDLPPYK
SYEQLKEKLLFAIEETEGFGQE*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Nucleotide sequence difference compared with NM_031483 CDS:
t1809c (silent in F603)

Estimated MW of expressed protein : 99859 Daltons

100042 R 1000 1001 1047 19007

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