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ISG15

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Plasmid
pGEX6P-1-ISG15 1-157
Parent Plasmid
pGEX6P1
Expressed
GST ISG15 1-157
DU Number
DU32336
Genbank
NP_005092
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGGGCTGGGACCTGACGGTGAAGATGCTGGCGGGCAACGAATT
CCAGGTGTCCCTGAGCAGCTCCATGTCGGTGTCAGAGCTGAAGGCGCAGA
TCACCCAGAAGATCGGCGTGCACGCCTTCCAGCAGCGTCTGGCTGTCCAC
CCGAGCGGTGTGGCGCTGCAGGACAGGGTCCCCCTTGCCAGCCAGGGCCT
GGGCCCCGGCAGCACGGTCCTGCTGGTGGTGGACAAATGCGACGAACCTC
TGAACATCCTGGTGAGGAATAACAAGGGCCGCAGCAGCACCTACGAGGTG
CGGCTGACGCAGACCGTGGCCCACCTGAAGCAGCAAGTGAGCGGGCTGGA
GGGTGTGCAGGACGACCTGTTCTGGCTGACCTTCGAGGGGAAGCCCCTGG
AGGACCAGCTCCCGCTGGGGGAGTACGGCCTCAAGCCCCTGAGCACCGTG
TTCATGAATCTGCGCCTGCGGGGAGGCTAAACAGAGCCTGGCGGGGAGGC
TAAACAGAGCCTGGCGGGGAGGCTAAACAGAGCCTGGCGGGGAGG
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGWDLTVKMLAGNEFQVSL
SSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGS
TVLLVVDKCDEPLNILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQD
DLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N ter GST for bacterial expression
Estimated MW of expressed protein : 43996 Daltons
100042 R 1000 1001 1047 19007
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