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A20

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Plasmid
pGEX6P-2-A20 1-467
Parent Plasmid
pGEX6P2
Expressed
GST A20 1-467
DU Number
DU32913
Genbank
BC114480
Tag
GST
Species
Human
Sequence of Insert
GCGGCCGCGATGGCTGAACAAGTCCTTCCTCAGGCTTTGTATTTGAGCAA
TATGCGGAAAGCTGTGAAGATACGGGAGAGAACTCCAGAAGACATTTTTA
AACCTACTAATGGGATCATTCATCATTTTAAAACCATGCACCGATACACA
CTGGAAATGTTCAGAACTTGCCAGTTTTGTCCTCAGTTTCGGGAGATCAT
CCACAAAGCCCTCATCGACAGAAACATCCAGGCCACCCTGGAAAGCCAGA
AGAAACTCAACTGGTGTCGAGAAGTCCGGAAGCTTGTGGCGCTGAAAACG
AACGGTGACGGCAATTGCCTCATGCATGCCACTTCTCAGTACATGTGGGG
CGTTCAGGACACAGACTTGGTACTGAGGAAGGCGCTGTTCAGCACGCTCA
AGGAAACAGACACACGCAACTTTAAATTCCGCTGGCAACTGGAGTCTCTC
AAATCTCAGGAATTTGTTGAAACGGGGCTTTGCTATGATACTCGGAACTG
GAATGATGAATGGGACAATCTTATCAAAATGGCTTCCACAGACACACCCA
TGGCCCGAAGTGGACTTCAGTACAACTCACTGGAAGAAATACACATATTT
GTCCTTTGCAACATCCTCAGAAGGCCAATCATTGTCATTTCAGACAAAAT
GCTAAGAAGTTTGGAATCAGGTTCCAATTTCGCCCCTTTGAAAGTGGGTG
GAATTTACTTGCCTCTCCACTGGCCTGCCCAGGAATGCTACAGATACCCC
ATTGTTCTCGGCTATGACAGCCATCATTTTGTACCCTTGGTGACCCTGAA
GGACAGTGGGCCTGAAATCCGAGCTGTTCCACTTGTTAACAGAGACCGGG
GAAGATTTGAAGACTTAAAAGTTCACTTTTTGACAGATCCTGAAAATGAG
ATGAAGGAGAAGCTCTTAAAAGAGTACTTAATGGTGATAGAAATCCCCGT
CCAAGGCTGGGACCATGGCACAACTCATCTCATCAATGCCGCAAAGTTGG
ATGAAGCTAACTTACCAAAAGAAATCAATCTGGTAGATGATTACTTTGAA
CTTGTTCAGCATGAGTACAAGAAATGGCAGGAAAACAGCGAGCAGGGGAG
GAGAGAGGGGCACGCCCAGAATCCCATGGAACCTTCCGTGCCCCAGCTTT
CTCTCATGGATGTAAAATGTGAAACGCCCAACTGCCCCTTCTTCATGTCT
GTGAACACCCAGCCTTTATGCCATGAGTGCTCAGAGAGGCGGCAAAAGAA
TCAAAACAAACTCCCAAAGCTGAACTCCAAGCCGGGCCCTGAGGGGCTCC
CTGGCATGGCGCTCGGGGCCTCTCGGGGAGAAGCCTATGAGCCCTTGGCG
TGGAACCCTGAGGAGTCCACTGGGGGGCCTCATTCGGCCCCACCGACAGC
ACCCAGCCCTTAACTGTTCAGTGAGACCACTGCCATGAAGTGCAGGAGCC
CCGGCTGCCCCTTCACACTGAATGTGCAGCACAACGGATTTTGTGAACGT
TGCCACAACGCCCGGCAACTTCACGCCAGCCACGCCCCAGACCACACAAG
GCACTTGGATCCCGGGAAGTGCCAAGCCTGCCTCCAGGATGTTACCAGGA
CATTTAATGGGATCTGCAGTACTTGCTTCAAAAGGACTACAGCAGAGGCC
TCCTCCAGCCTCAGCACCAGCCTCCCTCCTTCCTGTCACCAGCGTTCCAA
GTCAGATCCCTCGCGGCTCGTCCGGAGCCCCTCCCCGCATTCTTGCCACA
GAGCTGGAAACGACGCCCCTGCTGGCTGCCTGTCTCAAGCTGCACGGACT
CCTGGGGACAGGACGGGGACGAGCAAGTGCAGAAAAGCCGGCTGCGTGTA
TTTTGGGACTCCAGAAAACAAGGGCTTTTGCACACTGTGTTTCATCGAGT
ACAGAGAAAACAAACATTTTGCTGCTGCCTCAGGGAAAGTCAGTCCCACA
GCGTCCAGGTTCCAGAACACCATTCCGTGCCTGGGGAGGGAATGCGGCAC
CCTTGGAAGCACCATGTTTGAAGGATACTGCCAGAAGTGTTTCATTGAAG
CTCAGAATCAGAGATTTCATGAGGCCAAAAGGACAGAAGAGCAACTGAGA
TCGAGCCAGCGCAGAGATGTGCCTCGAACCACACAAAGCACCTCAAGGCC
CAAGTGCGCCCGGGCCTCCTGCAAGAACATCCTGGCCTGCCGCAGCGAGG
AGCTCTGCATGGAGTGTCAGCATCCCAACCAGAGGATGGGCCCTGGGGCC
CACCGGGGTGAGCCTGCCCCCGAAGACCCCCCCAAGCAGCGTTGCCGGGC
CCCCGCCTGTGATCATTTTGGCAATGCCAAGTGCAACGGCTACTGCAACG
AATGCTTTCAGTTCAAGCAGATGTATGGCTAAGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMAEQVLPQ
ALYLSNMRKAVKIRERTPEDIFKPTNGIIHHFKTMHRYTLEMFRTCQFCP
QFREIIHKALIDRNIQATLESQKKLNWCREVRKLVALKTNGDGNCLMHAT
SQYMWGVQDTDLVLRKALFSTLKETDTRNFKFRWQLESLKSQEFVETGLC
YDTRNWNDEWDNLIKMASTDTPMARSGLQYNSLEEIHIFVLCNILRRPII
VISDKMLRSLESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSHHFV
PLVTLKDSGPEIRAVPLVNRDRGRFEDLKVHFLTDPENEMKEKLLKEYLM
VIEIPVQGWDHGTTHLINAAKLDEANLPKEINLVDDYFELVQHEYKKWQE
NSEQGRREGHAQNPMEPSVPQLSLMDVKCETPNCPFFMSVNTQPLCHECS
ERRQKNQNKLPKLNSKPGPEGLPGMALGASRGEAYEPLAWNPEESTGGPH
SAPPTAPSP*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N ter GST for bacterial expression

Estimated MW of expressed protein : 81606 Daltons
100042 R 1000 1001 1047 19007
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