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FAN1 (C.elegans) (C01G5.8)

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Plasmid
pMal.His.FAN1 C.elegans
Expressed
MBP-FAN1 C.elegans
DU Number
DU19998
Genbank
NM_068596.3
Tag
MBP
Species
Caenorhabditis elegans
Sequence of Insert
GGATCCATGAAGAAAGCCAAGAAAGAGAAGAAAGAAAAGATCATTAAGCCAGACGGACCCATTAACTTTAATCGGAGTATCATGGCCGCTTTCGAGAAACAGAGCCGGGGCAAGATTTGCCCTCTGTGCGAAACTAAATTTTCACTGGCCAGCTACAAGTCCCACATGAACACCTGCAATGTGGCTGACGATGACGAGGAAATCGAAGTGATTGCCACATATACTCGCGACGAGGCTATCCTGATGAGGGCTGGACCAGAAATCATTCTGGGGGATGCCTCCTTCTCTGACAAGAGCGAGAACCCCACCAAACGGAGAAAGACAGATGAGCGGGAAGTGCCATCAGAGGATGACATTGTGCCCGAAGTGCCCGGACCTAGCGGCATCGTGAAGAACCACGAGATGCCTAGTGAATCACTGGACGTGACCGAGATCAGCGAAAATATTGAGAAAGTGATCAAGAAGAGCCCAGAGTGGATCAACCATCGGCGGCGGAGCAGCCGGCTGCTCCAGAACAGCCAGAAAGACCAGGCCGATAACGCTAATAAGGAGGACCCAGTGAAGAAAGAAACCGCCACAATTTCTGAGGTGCTCCAGGCTATCGAACGCTTCGAGCAGCGGGTGAGTGGACCAGAGCAGACATGGCCTTACTACATCAAAATCACTATCAAAATCATGAAGAGAGTGATCAGCACTGAGAAGTTCGACGGGACCTTTTACGCCGATGACTTTTGGCTGCCCTCCGATATCATTACATTCTATCGCTTTGTGGAGCTGCTGTCTGAAGGAGCTAAATGTCTGCTGGTGAGACTGTTCATCAGGAAGCCTGCCTGGTACAACCTGGAAAAACTGGAGCAGAAGTATCCTGAGATCCCAAATATCAAGGAGGCCGTGTCCGAACTGGCTAAGGGACACTTCATCGATGACGATTCTAGTATGAAAACCCTGGATGAGGCCCTCCAGATCAGCGACGTGGTGGCTCTGAAGAACGTGACCAAGAAATTCAAACTGGATGGCACAAAGAATCGCCAGGAGCTGATCCAGTCCCTGCGGAAATTTGCCCAGTCTCAGCAGAGTATTTTCGGCGGGACCGGCAACGTGGAGAAGAGCATCCTGAAATCCCTGAAGCAGGAACTGGGACCATGCGTGAGAGTGAGGGGAGGATTCGTGGACCTGTTTAAGTGCCTGTTCACAATCTACTGTCCCGTGACCACAAACTCAGCCAATGTGATCGACAACCCTAGCACTACCAACGTGTACCAGGATCTGCTGTATCTGATGCTGTCCGTGGCCAACGGGACAGTGCAGTTTCCAGCTCCCAATCCTTGTCCAATCATTGCCTCTTTCTACAAAAACAGGAATATGCTCCAGGATTATATGATCAGCAAGTCCCTGGAGATCGCCATCGTGAGCCAGATGAGCAACGGAAATCTGGACGCCGCTCTGGATCTGGCCATTGACGCTAAAGAGTTCATCGAGCAGATGAGCGACGATGACAAGAGATACTATGAATCCCTGGAGATTCACGAAAGGAAGTTTACAAGCATCTGGGTGTTCACTCGCTGCTGTGGGCATGCTTCAAGCATCCTGGAGCGGCAGAAGAAATACGGAATGGCCGTGGAATGGCAGAAGGACCTGCTGATTACCAACAAGGACATCCAGTCTTATTGCATTGATAGTCGCGGCATCTGGTGGGACCGGATGCTGCTGAATCTGGATAGTCACCTGAAAGAGAAGAAAGAATGTGCCAAGATGATCCAGATCGCCCTCCAGGACCCCTCTATTCTGGAGAAGGAACTGCTGATGATCCAGGACAGGGCTCTGAAGCTGAAAGAGATGCCAGCCGATTTCGTGACACCCATCAACATTGGGAATCCTGAAAAGAAAACTATTACCGCCAACGTGATCACTAAGAGCCTGGGGGACGGACGCATTAATCGGTTTATGATCCGCGATCACGAAACTGATGACGATGTGGAGTGCAGCGTGGAGGAAGTGACCAGAAGGCATTACCTGGAGAACGAAGGGTTCTCCACAGGAGTGCACGATGAGGGATCTACATGGCATACTCTGTTTGGCCTGTTCTTTTATGATGTGATCTTCGCCACTGACGAGTCCGTGGAATCTACCTGGCTGTCAGAGCTGCAAGATTGCCCAAGTGACCTGTCAAACACTCTGTACAGCAAGAGAAAAGAGAAGTTTGAAGACAGGTTCGTGTGGCTGGAGGAAGCTGAGCAGGAACTGATCGAGGAAAACATCAGGAAAATTTGGGACCTGAAGCACAACGAGACCAATCGCGAATGTAGTTGGAAGCAGTTCCCTATGGGCGCCGAGGATTGCGTGTCATTCTTTCAGTGTATCCCCCGGCCTGCTCTGATCCTGATTCTGCGCCGGCTGGCCGAGAACTATAGAAATTCTAGGAGTGGGTTTCCTGACCTGACCCTGTGGAACCCAGAAACAAAAAGAGTGGCTGTGGTGGAGGTGAAGGGACCAGGGGACAGACTGAGCACCAAGCAGAGGCTGTGGCTGGCCATTTTCGCTGATTCCGGCATCCGGGCTGAGGTGTGCCATGTGGCTGCTCAGAACAGCCGGCTGCTGGTGTGAGCGGCCGC
Amino Acid Sequence
MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGLEVLFQGPLGSMKKAKKEKKEKIIKPDGPINFNRSIMAAFEKQSRGKICPLCETKFSLASYKSHMNTCNVADDDEEIEVIATYTRDEAILMRAGPEIILGDASFSDKSENPTKRRKTDEREVPSEDDIVPEVPGPSGIVKNHEMPSESLDVTEISENIEKVIKKSPEWINHRRRSSRLLQNSQKDQADNANKEDPVKKETATISEVLQAIERFEQRVSGPEQTWPYYIKITIKIMKRVISTEKFDGTFYADDFWLPSDIITFYRFVELLSEGAKCLLVRLFIRKPAWYNLEKLEQKYPEIPNIKEAVSELAKGHFIDDDSSMKTLDEALQISDVVALKNVTKKFKLDGTKNRQELIQSLRKFAQSQQSIFGGTGNVEKSILKSLKQELGPCVRVRGGFVDLFKCLFTIYCPVTTNSANVIDNPSTTNVYQDLLYLMLSVANGTVQFPAPNPCPIIASFYKNRNMLQDYMISKSLEIAIVSQMSNGNLDAALDLAIDAKEFIEQMSDDDKRYYESLEIHERKFTSIWVFTRCCGHASSILERQKKYGMAVEWQKDLLITNKDIQSYCIDSRGIWWDRMLLNLDSHLKEKKECAKMIQIALQDPSILEKELLMIQDRALKLKEMPADFVTPINIGNPEKKTITANVITKSLGDGRINRFMIRDHETDDDVECSVEEVTRRHYLENEGFSTGVHDEGSTWHTLFGLFFYDVIFATDESVESTWLSELQDCPSDLSNTLYSKRKEKFEDRFVWLEEAEQELIEENIRKIWDLKHNETNRECSWKQFPMGAEDCVSFFQCIPRPALILILRRLAENYRNSRSGFPDLTLWNPETKRVAVVEVKGPGDRLSTKQRLWLAIFADSGIRAEVCHVAAQNSRLLV*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial construct to express Protein with N-ter MBP.

Estimated MW of expressed protein : 145105.75 Daltons

All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.
100042 R 1000 1001 1047 19007
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