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Plasmid
pGEX6P-1-HOIP R480-D636 V492E/Q613A/L617A/F620A
Parent Plasmid
pGEX6P-1
Expressed
GST-HOIP R480-D636 V492E/Q613A/L617A/F620A
DU Number
DU65912
Genbank
NM_017999.5
Tag
GST
Species
Human
Sequence of Insert
View Sequence
ggatccCGCCAAGACAAGATGCGGGAAGAAGGCCTCCAGCTAGaGAGCATGATCCGGGAAGGGGAAGCCGCAGGTGCCTGTCCAGAGGAGATCTTCTCGGCTCTGCAGTACTCGGGCACTGAGGTGCCTCTGCAGTGGTTGCGCTCAGAACTGCCCTACGTCCTGGAGATGGTGGCTGAGCTGGCTGGACAGCAGGACCCTGGGCTGGGTGCCTTTTCCTGTCAGGAGGCCCGGAGAGCCTGGCTGGATCGTCATGGCAACCTTGATGAAGCTGTGGAGGAGTGTGTGAGGACCAGGCGAAGGAAGGTGCAGGAGCTCCAGTCTCTAGGCTTTGGGCCTGAGGAGGGGTCTCTCCAGGCATTGTTCCAGCACGGAGGTGATGTGTCACGGGCCCTGACTGAGCTAgcGCGCCAACGCgcAGAGCCCgcCCGCCAGCGCCTCTGGGACAGTGGCCCTGAGCCCACCCCTTCCTGGGATtgagcggccgc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSRQDKMREEGLQLESMIREG
EAAGACPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGA
FSCQEARRAWLDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQAL
FQHGGDVSRALTELARQRAEPARQRLWDSGPEPTPSWD*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSRQDKMREEGLQLESMIREG
EAAGACPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGA
FSCQEARRAWLDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQAL
FQHGGDVSRALTELARQRAEPARQRLWDSGPEPTPSWD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N-term GST tag for bacterial expression of D-UBA domain of HOIP.
100042 R 1000 1001 1047 19007
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