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UBC12

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Plasmid
pCDF-PylT-6HIS(TAG3)-Ubc12
Parent Plasmid
pCDF-PylT
Expressed
6HIS(TAG3)-Ubc12
DU Number
DU24657
Genbank
P691081
Tag
6His
Species
Human
Sequence of Insert
CCATGGGCTAGAGCCATCATCATCATCATCACAGCAGCGGCCTGGAAGTT
CTGTTCCAGGGGCCCGGATCCCCGGAATTCCCGGGGGTCGACTCGAAAGC
GGCCGCGATGATCAAGCTGTTCTCGCTGAAGCAGCAGAAGAAGGAGGAGG
AGTCGGCGGGCGGCACCAAGGGCAGCAGCAAGAAGGCGTCGGCGGCGCAG
CTGCGGATCCAGAAGGACATAAACGAGCTGAACCTGCCCAAGACGTGTGA
TATCAGCTTCTCAGATCCAGACGACCTCCTCAACTTCAAGCTGGTCATCT
GTCCTGATGAGGGCTTCTACAAGAGTGGGAAGTTTGTGTTCAGTTTTAAG
GTGGGCCAGGGTTACCCGCATGATCCCCCCAAGGTGAAGTGTGAGACAAT
GGTCTATCACCCCAACATTGACCTCGAaGGCAACGTCTGCCTCAACATCC
TCAGAGAGGACTGGAAGCCAGTCCTTACGATAAACTCCATAATTTATGGC
CTGCAGTATCTCTTCTTGGAGCCCAACCCCGAGGACCCACTGAACAAGGA
GGCCGCAGAGGTCCTGCAGAACAACCGGCGGCTGTTTGAGCAGAACGTGC
AGCGCTCCATGCGGGGTGGCTACATCGGCTCCACCTACTTTGAGCGCTGC
CTGAAATAACTCGAG
Amino Acid Sequence
MG*SHHHHHHSSGLEVLFQGPGSPEFPGVDSKAAAMIKLFSLKQQKKEEE
SAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVIC
PDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNIL
REDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQ
RSMRGGYIGSTYFERCLK*
Vector Type
Bacterial
Antibiotic
Spectinomycin
Comments
internal Xho1 at nt427 killed
100042 R 1000 1001 1047 19007
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