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Synonyms
YHR1
Plasmid
pRS413-ADH Pr-3HA-Uba4 C225S
Parent Plasmid
pRS413 pADH 3HA
Expressed
3HA-Uba4 C225S
DU Number
DU24122
Genbank
NM_001179241.1
Tag
3HA
Species
Saccharomyces cerevisiae
Sequence of Insert
View Sequence
gaattcATGAATGACTACCATCTCGAGGATACCACGTCTGAACTTGAAGCATTAAG
ATTGGAGAATGCACAATTAAGAGAGCAGCTTGCCAAGAGAGAAGACAGCA
GCCGCGACTACCCATTATCTTTGGAAGAATACCAACGTTACGGAAGACAA
ATGATTGTTGAAGAAACAGGTGGTGTAGCAGGTCAAGTCAAGTTGAAAAA
TACAAAAGTTTTGGTAGTTGGTGCTGGAGGTTTGGGATGTCCCGCCTTGC
CCTACTTAGCGGGCGCTGGCGTGGGCCAAATAGGCATAGTAGATAACGAT
GTGGTGGAGACTTCCAATTTGCATAGGCAGGTTCTTCATGATTCTAGCAG
AGTTGGAATGTTGAAATGTGAGTCGGCCAGGCAATATATCACGAAACTGA
ACCCACACATTAACGTCGTTACCTATCCCGTTAGATTGAACTCCAGTAAT
GCTTTTGACATTTTCAAAGGTTACAATTATATATTAGACTGCACAGATTC
TCCATTAACGAGATACCTGGTGTCTGATGTTGCAGTTAATCTGGGAATAA
CAGTAGTGTCTGCATCCGGTTTGGGAACAGAGGGCCAGCTAACTATATTG
AATTTTAACAATATAGGGCCATGCTACAGATGCTTTTATCCAACACCTCC
GCCACCAAATGCCGTGACCTCTTcCCAAGAAGGCGGTGTGATAGGACCTT
GCATTGGACTAGTTGGAACAATGATGGCTGTAGAAACTTTGAAGCTTATC
CTAGGAATCTACACCAATGAAAATTTTAGTCCCTTTTTGATGTTATATTC
AGGTTTCCCACAGCAGAGCCTGCGCACTTTCAAAATGAGAGGCAGACAAG
AAAAGTGTCTATGCTGTGGTAAGAATCGAACGATAACAAAAGAAGCCATC
GAAAAAGGTGAGATCAATTACGAACTGTTTTGTGGCGCACGAAACTATAA
TGTATGCGAGCCTGATGAGAGAATCAGTGTGGACGCATTTCAGCGTATCT
ACAAGGATGATGAATTTCTAGCAAAACATATATTTCTTGATGTTAGGCCA
TCCCATCATTACGAGATATCTCATTTCCCTGAAGCAGTTAATATTCCAAT
CAAAAATCTAAGAGATATGAACGGTGATCTTAAGAAACTGCAAGAAAAAC
TTCCCAGTGTAGAAAAAGACAGTAATATAGTGATTCTTTGCCGCTACGGT
AACGACTCTCAGCTAGCTACAAGATTATTGAAGGATAAATTTGGGTTTTC
TAATGTACGAGACGTGAGAGGAGGATACTTCAAATACATAGACGATATTG
ATCAAACCATTCCTAAATATTAGgtcgac
ATTGGAGAATGCACAATTAAGAGAGCAGCTTGCCAAGAGAGAAGACAGCA
GCCGCGACTACCCATTATCTTTGGAAGAATACCAACGTTACGGAAGACAA
ATGATTGTTGAAGAAACAGGTGGTGTAGCAGGTCAAGTCAAGTTGAAAAA
TACAAAAGTTTTGGTAGTTGGTGCTGGAGGTTTGGGATGTCCCGCCTTGC
CCTACTTAGCGGGCGCTGGCGTGGGCCAAATAGGCATAGTAGATAACGAT
GTGGTGGAGACTTCCAATTTGCATAGGCAGGTTCTTCATGATTCTAGCAG
AGTTGGAATGTTGAAATGTGAGTCGGCCAGGCAATATATCACGAAACTGA
ACCCACACATTAACGTCGTTACCTATCCCGTTAGATTGAACTCCAGTAAT
GCTTTTGACATTTTCAAAGGTTACAATTATATATTAGACTGCACAGATTC
TCCATTAACGAGATACCTGGTGTCTGATGTTGCAGTTAATCTGGGAATAA
CAGTAGTGTCTGCATCCGGTTTGGGAACAGAGGGCCAGCTAACTATATTG
AATTTTAACAATATAGGGCCATGCTACAGATGCTTTTATCCAACACCTCC
GCCACCAAATGCCGTGACCTCTTcCCAAGAAGGCGGTGTGATAGGACCTT
GCATTGGACTAGTTGGAACAATGATGGCTGTAGAAACTTTGAAGCTTATC
CTAGGAATCTACACCAATGAAAATTTTAGTCCCTTTTTGATGTTATATTC
AGGTTTCCCACAGCAGAGCCTGCGCACTTTCAAAATGAGAGGCAGACAAG
AAAAGTGTCTATGCTGTGGTAAGAATCGAACGATAACAAAAGAAGCCATC
GAAAAAGGTGAGATCAATTACGAACTGTTTTGTGGCGCACGAAACTATAA
TGTATGCGAGCCTGATGAGAGAATCAGTGTGGACGCATTTCAGCGTATCT
ACAAGGATGATGAATTTCTAGCAAAACATATATTTCTTGATGTTAGGCCA
TCCCATCATTACGAGATATCTCATTTCCCTGAAGCAGTTAATATTCCAAT
CAAAAATCTAAGAGATATGAACGGTGATCTTAAGAAACTGCAAGAAAAAC
TTCCCAGTGTAGAAAAAGACAGTAATATAGTGATTCTTTGCCGCTACGGT
AACGACTCTCAGCTAGCTACAAGATTATTGAAGGATAAATTTGGGTTTTC
TAATGTACGAGACGTGAGAGGAGGATACTTCAAATACATAGACGATATTG
ATCAAACCATTCCTAAATATTAGgtcgac
Amino Acid Sequence
View Sequence
MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFMNDYHLEDTTSELEALRL
ENAQLREQLAKREDSSRDYPLSLEEYQRYGRQMIVEETGGVAGQVKLKNT
KVLVVGAGGLGCPALPYLAGAGVGQIGIVDNDVVETSNLHRQVLHDSSRV
GMLKCESARQYITKLNPHINVVTYPVRLNSSNAFDIFKGYNYILDCTDSP
LTRYLVSDVAVNLGITVVSASGLGTEGQLTILNFNNIGPCYRCFYPTPPP
PNAVTSSQEGGVIGPCIGLVGTMMAVETLKLILGIYTNENFSPFLMLYSG
FPQQSLRTFKMRGRQEKCLCCGKNRTITKEAIEKGEINYELFCGARNYNV
CEPDERISVDAFQRIYKDDEFLAKHIFLDVRPSHHYEISHFPEAVNIPIK
NLRDMNGDLKKLQEKLPSVEKDSNIVILCRYGNDSQLATRLLKDKFGFSN
VRDVRGGYFKYIDDIDQTIPKY*
ENAQLREQLAKREDSSRDYPLSLEEYQRYGRQMIVEETGGVAGQVKLKNT
KVLVVGAGGLGCPALPYLAGAGVGQIGIVDNDVVETSNLHRQVLHDSSRV
GMLKCESARQYITKLNPHINVVTYPVRLNSSNAFDIFKGYNYILDCTDSP
LTRYLVSDVAVNLGITVVSASGLGTEGQLTILNFNNIGPCYRCFYPTPPP
PNAVTSSQEGGVIGPCIGLVGTMMAVETLKLILGIYTNENFSPFLMLYSG
FPQQSLRTFKMRGRQEKCLCCGKNRTITKEAIEKGEINYELFCGARNYNV
CEPDERISVDAFQRIYKDDEFLAKHIFLDVRPSHHYEISHFPEAVNIPIK
NLRDMNGDLKKLQEKLPSVEKDSNIVILCRYGNDSQLATRLLKDKFGFSN
VRDVRGGYFKYIDDIDQTIPKY*
Vector Type
Other
Antibiotic
Amp
Comments
chromosome VIII coordinates 333072 to 334394
100042 R 1000 1001 1047 19007
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