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Synonyms
E2-230K
Plasmid
pFBDual DACTEV (not blunt)-TRIM27/6His-C3-UBE20
Parent Plasmid
pFastbacDual
Expressed
6His-C3-UBE20
DU Number
DU45791
Genbank
NM_022066.3 GI:192449448 with silent change g6a (to kill BamH1) and coding change g3619a (G1207S) - common variant
Tag
6His
Species
Human
Sequence of Insert
View Sequence
ATGCATGAAACCATGTCGTACTACCATCACCATCACCATCACCTGGAAGTTCTGTTCCAGGGGCCCATGGCAGATCCCGCAGCCCCCACGCCCGCAGCTCCCGCTCCAGCCCAGGCCCCGGCTCCAGCCCCGGAGGCAGTCCCGGCCCCAGCCGCAGCCCCCGTCCCGGCGCCGGCGCCCGCCTCGGACTCGGCCTCCGGGCCGTCCTCGGACTCCGGCCCAGAAGCCGGCTCGCAGCGCCTGCTGTTTTCTCACGACCTGGTGTCGGGCCGTTACCGTGGCTCCGTGCACTTCGGGCTGGTGCGCCTCATCCACGGCGAGGACTCGGACTCGGAGGGCGAGGAGGAGGGCCGCGGGAGCTCGGGGTGCTCCGAGGCCGGGGGCGCGGGCCACGAGGAGGGCCGGGCCAGCCCCCTGCGCCGCGGCTACGTGCGCGTCCAGTGGTACCCGGAGGGCGTCAAGCAGCATGTGAAGGAGACCAAGCTGAAACTAGAGGACCGTTCTGTGGTGCCCCGAGATGTGGTCCGGCACATGCGATCCACCGACAGTCAGTGTGGCACGGTGATCGACGTCAACATCGACTGTGCCGTCAAGCTCATCGGCACCAACTGCATCATCTATCCCGTCAACAGCAAGGACCTGCAGCACATCTGGCCCTTCATGTATGGGGACTACATTGCCTATGACTGCTGGCTGGGGAAGGTCTACGACTTGAAGAACCAGATCATCCTGAAGCTATCCAACGGCGCCAGGTGCTCCATGAACACGGAAGATGGCGCCAAGCTCTACGACGTCTGCCCGCACGTCAGCGACTCGGGTCTCTTCTTCGATGATTCCTATGGCTTCTACCCAGGCCAGGTGCTCATTGGCCCTGCCAAGATCTTCTCCAGCGTCCAGTGGCTGTCAGGTGTCAAGCCCGTGCTCAGCACCAAGAGCAAGTTCCGAGTGGTGGTGGAAGAGGTGCAGGTTGTAGAGTTGAAAGTTACATGGATTACCAAGAGTTTCTGTCCAGGGGGCACGGACAGCGTCAGCCCCCCACCCTCTGTCATCACCCAGGAAAACCTAGGCAGGGTGAAGCGTCTCGGATGCTTTGACCATGCTCAGCGGCAGCTTGGGGAGCGCTGTCTGTATGTCTTCCCAGCCAAGGTAGAGCCAGCCAAGATTGCCTGGGAATGTCCAGAAAAAAACTGCGCCCAGGGGGAGGGCTCTATGGCCAAGAAGGTGAAGCGCCTGTTGAAGAAGCAGGTTGTGCGGATCATGTCATGCTCCCCAGACACCCAGTGTTCCCGGGACCATTCCATGGAAGACCCAGACAAGAAGGGGGAATCCAAAACCAAGAGCGAAGCGGAGTCTGCCAGCCCTGAGGAGACGCCCGATGGCTCTGCCAGTCCAGTGGAGATGCAGGACGAGGGTGCAGAGGAGCCCCACGAGGCAGGAGAGCAGCTGCCCCCATTCCTGCTAAAAGAAGGCAGAGATGACAGGCTGCACTCGGCAGAGCAGGACGCAGATGATGAGGCTGCTGATGACACGGACGACACCAGTTCGGTGACCTCCTCTGCCAGCTCCACCACTTCCTCCCAGAGCGGCAGCGGCACGAGTCGCAAAAAGAGCATCCCCTTGTCCATCAAGAACTTAAAGCGCAAACACAAGAGGAAGAAGAATAAAATCACTCGAGACTTCAAGCCAGGGGACAGGGTGGCAGTGGAGGTGGTGACCACGATGACCTCAGCCGACGTGATGTGGCAGGATGGCTCCGTGGAATGCAACATCCGCTCCAACGACCTCTTCCCTGTGCACCACCTGGACAACAACGAGTTCTGCCCTGGAGACTTCGTGGTAGATAAGCGAGTCCAGAGCTGTCCAGACCCTGCTGTCTACGGTGTGGTACAGTCTGGGGACCACATCGGCCGTACCTGCATGGTGAAGTGGTTCAAGCTGAGGCCGAGTGGGGACGACGTGGAGCTGATTGGAGAAGAGGAAGATGTGAGTGTTTACGACATTGCTGACCACCCTGACTTTAGGTTCCGTACAACTGACATCGTCATCCGCATCGGCAATACTGAGGATGGGGCTCCTCACAAGGAGGATGAGCCATCGGTGGGCCAGGTGGCCCGTGTGGACGTCAGCAGCAAGGTGGAGGTGGTGTGGGCTGACAACTCAAAGACCATCATCCTGCCCCAGCACTTGTACAACATAGAGTCTGAGATTGAGGAGTCAGACTACGATTCGGTAGAAGGCAGCACCAGCGGGGCATCCTCGGATGAATGGGAAGATGATAGTGACAGCTGGGAGACGGACAATGGGCTGGTGGAGGACGAGCACCCCAAGATAGAGGAGCCCCCCATCCCACCCCTGGAGCAGCCGGTGGCCCCTGAGGACAAGGGAGTGGTGATCAGTGAAGAGGCAGCCACAGCTGCCGTCCAGGGGGCTGTGGCCATGGCTGCCCCCATGGCCGGGCTGATGGAGAAGGCTGGCAAGGACGGGCCACCCAAGAGCTTCCGGGAGTTGAAAGAGGCCATCAAGATCCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGCTGACGGGCTCGCCCACCTCTCCGACTGTGGAGCCTGAGAAGCCAACTCGGGAGAAGAAGTTTCTGGATGACATCAAGAAGCTACAGGAAAACCTCAAGAAGACCCTGGACAATGTGGCCATTGTAGAGGAGGAGAAGATGGAAGCAGTGCCCGACGTAGAGCGCAAGGAGGACAAGCCCGAGGGGCAGTCACCTGTGAAGGCTGAGTGGCCCAGCGAAACCCCGGTGCTGTGCCAGCAGTGTGGCGGCAAGCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTCTCCGTACTGGAGTTTGCACCCTCAAATCATTCTTTTAAGAAAATTGAGTTCCAGCCTCCAGAAGCCAAGAAGTTCTTCAGCACAGTGCGGAAGGAGATGGCGCTGCTGGCTACCTCACTGCCTGAGGGCATCATGGTCAAGACTTTTGAAGATAGAATGGACCTCTTCTCAGCTCTCATCAAGGGCCCCACTCGAACCCCCTACGAGGATGGCCTCTACTTGTTTGACATCCAGCTCCCCAACATCTACCCAGCCGTGCCCCCCCACTTCTGCTACCTCTCCCAATGCAGTGGCCGCCTGAACCCCAACCTGTATGACAATGGGAAGGTGTGTGTCAGCCTCCTGGGCACCTGGATTGGAAAGGGGACAGAGAGGTGGACAAGCAAGTCCAGCCTTCTCCAGGTGCTCATCTCCATCCAAGGTCTGATCCTGGTAAATGAACCATACTACAACGAAGCCGGCTTCGACAGTGACCGAGGCCTGCAGGAAGGCTATGAAAACAGTCGCTGTTACAATGAGATGGCGCTGATCCGCGTGGTGCAGTCCATGACCCAGCTGGTGCGGCGGCCCCCCGAGGTCTTTGAGCAGGAGATCAGGCAACACTTTAGCACTGGTGGCTGGCGGCTGGTGAACCGTATCGAGTCCTGGCTGGAAACCCATGCCCTGCTGGAGAAGGCCCAGGCACTGCCCAACGGGGTGCCCAAGGCCAGCAGCTCGCCAGAGCCCCCAGCTGTAGCCGAGCTGTCAGACTCCGGCCAACAAGAACCTGAGGATGGAGGGCCAGCCCCAGGAGAGGCCTCCCAGGGCTCAGACTCAGAGGGCGGTGCCCAGAGCCTGGCCTCAGCTAGCAGGGACCACACAGACCAGACTTCGGAGACCGCACCAGACGCATCGGTGCCACCCAGTGTGAAACCAAAGAAGCGGAGAAAGAGCTACCGGAGCTTCTTACCTGAGAAGAGTGGCTACCCTGACATCGGCTTCCCCCTCTTCCCACTTTCCAAGGGTTTCATCAAGAGCATCCGGGGTGTCCTGACGCAGTTCCGGGCTGCCCTGCTAGAGGCAGGCATGCCGGAGTGCACAGAGGACAAGTAGATGCAT
Amino Acid Sequence
View Sequence
MSYYHHHHHHLEVLFQGPMADPAAPTPAAPAPAQAPAPAPEAVPAPAAAP
VPAPAPASDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLI
HGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVK
QHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNC
IIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSM
NTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGV
KPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQEN
LGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSM
AKKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEAESASP
EETPDGSASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADD
EAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKN
KITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNN
EFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVE
LIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVA
RVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSD
EWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISEEAAT
AAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQL
LTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAV
PDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVL
EFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRM
DLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPN
LYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEA
GFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFS
TGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQ
QEPEDGGPAPGEASQGSDSEGGAQSLASASRDHTDQTSETAPDASVPPSV
KPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLE
AGMPECTEDK*
VPAPAPASDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLI
HGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVK
QHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNC
IIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSM
NTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGV
KPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQEN
LGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSM
AKKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEAESASP
EETPDGSASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADD
EAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKN
KITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNN
EFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVE
LIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVA
RVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSD
EWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISEEAAT
AAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQL
LTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAV
PDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVL
EFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRM
DLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPN
LYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEA
GFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFS
TGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQ
QEPEDGGPAPGEASQGSDSEGGAQSLASASRDHTDQTSETAPDASVPPSV
KPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLE
AGMPECTEDK*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
6His-C3-UBE20 from DU45751 cloned NsiI/NsiI into DU45714 at NsiI site.
100042 R 1000 1001 1047 19007
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