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Synonyms
E2-EPF; E2EPF; EPF5
Plasmid
pGEX6P UBE2S M1-E192
Parent Plasmid
pGEX6P1
Expressed
GST-UBE2S M1-E192
DU Number
DU45350
Genbank
NM_014501.2 GI:112382376 with silent changes g435a and c511t
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCATGAACTCCAACGTGGAGAACCTACCCCCGCACATCATCCGCCTGGTGTACAAGGAGGTGACGACACTGACCGCAGACCCACCCGATGGCATCAAGGTCTTTCCCAACGAGGAGGACCTCACCGACCTCCAGGTCACCATCGAGGGCCCTGAGGGGACCCCATATGCTGGAGGTCTGTTCCGCATGAAACTCCTGCTGGGGAAGGACTTCCCTGCCTCCCCACCCAAGGGCTACTTCCTGACCAAGATCTTCCACCCGAACGTGGGCGCCAATGGCGAGATCTGCGTCAACGTGCTCAAGAGGGACTGGACGGCTGAGCTGGGCATCCGACACGTACTGCTGACCATCAAGTGCCTGCTGATCCACCCTAACCCCGAGTCTGCACTCAACGAGGAGGCGGGCCGCCTGCTCTTGGAGAACTACGAGGAGTATGCAGCTCGGGCCCGTCTGCTCACAGAGATCCACGGGGGCGCCGGCGGGCCCAGCGGCAGGGCCGAAGCCGGTCGGGCCTTGGCCAGTGGCACTGAAGCTTCCTCCACCGACCCTGGGGCCCCAGGGGGCCCGGGAGGGGCTGAGTAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNSNVENLPPHIIRLVYKE
VTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKD
FPASPPKGYFLTKIFHPNVGANGEICVNVLKRDWTAELGIRHVLLTIKCL
LIHPNPESALNEEAGRLLLENYEEYAARARLLTEIHGGAGGPSGRAEAGR
ALASGTEASSTDPGAPGGPGGAE*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNSNVENLPPHIIRLVYKE
VTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKD
FPASPPKGYFLTKIFHPNVGANGEICVNVLKRDWTAELGIRHVLLTIKCL
LIHPNPESALNEEAGRLLLENYEEYAARARLLTEIHGGAGGPSGRAEAGR
ALASGTEASSTDPGAPGGPGGAE*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N-ter GST followed by C3 cleavage site; UBE2S residues 1-192
100042 R 1000 1001 1047 19007
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