UBE2J1

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Plasmid
pET28a-UBE2J1 1-282
Parent Plasmid
pET28a original
Expressed
6His-UBE2J1 1-282
DU Number
DU20686
Genbank
NM_016021
Tag
6His
Species
Human
Sequence of Insert
GGATCCATGGAGACCCGCTACAACCTGAAGAGTCCGGCTGTTAAACGTTT
AATGAAAGAAGCGGCAGAATTGAAAGATCCAACAGATCATTACCATGCGC
AGCCTTTAGAGGATAACCTTTTTGAATGGCACTTCACGGTTAGAGGGCCC
CCAGACTCCGATTTTGATGGAGGAGTTTATCACGGGCGGATAGTACTGCC
ACCAGAGTATCCCATGAAACCACCAAGCATTATTCTCCTAACGGCTAATG
GTCGATTTGAAGTGGGCAAGAAAATCTGTTTGAGCATCTCAGGCCATCAT
CCTGAAACTTGGCAGCCTTCGTGGAGTATAAGGACAGCATTATTAGCCAT
CATTGGGTTTATGCCAACAAAAGGAGAGGGAGCCATAGGTTCTCTAGATT
ACACTCCTGAGGAAAGAAGAGCACTTGCCAAAAAATCACAAGATTTCTGT
TGTGAAGGATGTGGCTCTGCCATGAAGGATGTCCTGTTGCCTTTAAAATC
TGGAAGCGATTCAAGCCAAGCTGACCAAGAAGCCAAAGAACTGGCTAGGC
AAATAAGCTTTAAGGCAGAAGTCAATTCATCTGGAAAGACTATCTCTGAG
TCAGACTTAAACCACTCTTTTTCACTAACTGATTTACAAGATGATATACC
TACAACATTCCAGGGTGCTACGGCCAGTACATCGTACGGACTCCAGAATT
CCTCAGCAGCATCCTTTCATCAACCTACCCAACCTGTAGCTAAGAATACC
TCCATGAGCCCTCGACAGCGCCGGGCCCAGCAGCAGAGTCAGAGAAGGTT
GTCTACTTCACCAGATGTAATCCAGGGCCACCAGCCAAGAGACAACCACA
CTTAGGCGGCCGC
Amino Acid Sequence
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMETRYNLKSPAVKRLM
KEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPP
EYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAII
GFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSG
SDSSQADQEAKELARQISFKAEVNSSGKTISESDLNHSFSLTDLQDDIPT
TFQGATASTSYGLQNSSAASFHQPTQPVAKNTSMSPRQRRAQQQSQRRLS
TSPDVIQGHQPRDNHT*
Vector Type
Bacterial
Antibiotic
Kan
Comments
Bacterial expression construct to express UBE2J1 aa 1-1 282 with N-terminal 6HIS tag (contains Thrombin cleavage site)

Estimated MW of expressed protein : 34709 Daltons
100042 R 1000 1001 1047 19007
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