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UBE2Q2

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Plasmid
pET28-UBE2Q2
Parent Plasmid
pET28a original
Expressed
6His-UBE2Q2
DU Number
DU20500
Genbank
AAH34342.1
Tag
6His
Species
Human
Sequence of Insert
GGATCCATGTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCGTC
CATCTTCGACAAGAACCACGAGCGATTCCGCATCGTCAGTTGGAAGCTGG
ACGAGCTGCACTGCCAGTTCCTGGTGCCGCAGCAGGGCAGCCCGCACTCG
CTGCCGCCGCCACTCACGCTCCACTGCAACATCACGGAATCCTATCCATC
TTCTTCACCGATATGGTTTGTGGATTCTGAAGACCCAAATCTGACATCAG
TTCTGGAACGTCTAGAAGATACTAAGAACAACAATTTGCTTCGTCAGCAA
TTGAAGTGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAGCA
CCTGGATGTTGAGATGCTAGATCAACCACTACCCACGGGTCAGAATGGGA
CAACAGAAGAAGTGACTTCAGAAGAAGAGGAAGAAGAAGAAGAGATGGCT
GAAGATATAGAAGACTTAGATCACTATGAGATGAAGGAAGAAGAGCCTAT
TAGTGGGAAAAAGTCAGAGGATGAAGGAATTGAAAAAGAAAATTTGGCAA
TATTAGAGAAAATTAGGAAGACTCAAAGGCAAGACCATTTAAATGGTGCA
GTGTCTGGGTCAGTGCAAGCTTCAGATAGACTTATGAAAGAGCTCAGGGA
CATATACAGATCACAGAGTTATAAAACAGGGATTTATTCAGTGGAACTCA
TAAATGACAGTTTATATGACTGGCATGTTAAACTGCAGAAGGTTGACCCT
GATAGTCCTTTGCACAGTGATCTTCAGATCTTAAAAGAAAAAGAAGGCAT
AGAATATATTTTGCTTAACTTCTCTTTTAAGGATAACTTTCCATTTGATC
CTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGAGGGTATGTATTG
GGTGGAGGAGCATTATGTATGGAACTTCTCACAAAACAGGGCTGGAGCAG
TGCCTACTCAATAGAATCGGTCATCATGCAAATAAATGCCACCTTAGTCA
AAGGCAAAGCCAGAGTGCAGTTTGGAGCAAATAAGAATCAATATAATCTA
GCAAGAGCCCAACAATCCTATAATTCCATTGTACAGATACATGAGAAAAA
TGGCTGGTACACCCCTCCAAAGGAAGATGGCTAAGCGGCCGC
Amino Acid Sequence
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMSVSGLKAELKFLASI
FDKNHERFRIVSWKLDELHCQFLVPQQGSPHSLPPPLTLHCNITESYPSS
SPIWFVDSEDPNLTSVLERLEDTKNNNLLRQQLKWLICELCSLYNLPKHL
DVEMLDQPLPTGQNGTTEEVTSEEEEEEEEMAEDIEDLDHYEMKEEEPIS
GKKSEDEGIEKENLAILEKIRKTQRQDHLNGAVSGSVQASDRLMKELRDI
YRSQSYKTGIYSVELINDSLYDWHVKLQKVDPDSPLHSDLQILKEKEGIE
YILLNFSFKDNFPFDPPFVRVVLPVLSGGYVLGGGALCMELLTKQGWSSA
YSIESVIMQINATLVKGKARVQFGANKNQYNLARAQQSYNSIVQIHEKNG
WYTPPKEDG*






Vector Type
Bacterial
Antibiotic
Kan
Comments
Bacterial expression construct to express UBE2Q2 with N-terminal 6HIS tag (contains Thrombin cleavage site)

Estimated MW of expressed protein : 46363 Daltons
100042 R 1000 1001 1047 19007
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