DCUN1D3

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Plasmid
pEBG6P-DCUN1D3
Parent Plasmid
pEBG6P
Expressed
GST-DCUN1D3
DU Number
DU20453
Genbank
NM_173475*
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGGGCCAGTGTGTCACCAAGTGTAAGAATCCCTCATCGACCCT
GGGCAGCAAAAATGGAGACCGTGAGCCCAGCAACAAGTCACATAGCAGGA
GGGGTGCAGGCCACCGTGAGGAGCAGGTACCACCCTGTGGCAAGCCAGGT
GGAGATATCCTCGTCAACGGGACCAAGAAGGCCGAGGCTGCCACTGAGGC
CTGCCAGCTGCCAACGTCCTCGGGAGATGCTGGGAGGGAGTCCAAGTCCA
ATGCCGAGGAGTCTTCCTTGCAAAGATTGGAAGAACTGTTCAGGCGCTAC
AAGGATGAGCGGGAAGATGCAATTTTGGAGGAAGGCATGGAGCGCTTTTG
CAATGACCTGTGTGTTGACCCCACAGAATTTCGAGTGCTGCTCTTGGCTT
GGAAGTTCCAGGCTGCAACCATGTGCAAATTCACCAGGAAGGAGTTTTTT
GATGGCTGCAAAGCAATAAGTGCAGACAGCATTGACGGAATCTGTGCACG
GTTCCCTAGCCTCTTAACAGAAGCCAAACAAGAGGATAAATTCAAGGATC
TCTACCGGTTTACATTTCAGTTTGGCCTGGACTCTGAAGAAGGGCAGCGG
TCACTGCATCGGGAAATAGCCATTGCCCTGTGGAAACTAGTCTTTACCCA
GAACAATCCTCCGGTATTGGACCAATGGCTAAACTTCCTAACAGAGAACC
CCTCGGGGATCAAGGGCATCTCCCGGGACACTTGGAACATGTTCCTTAAC
TTCACTCAGGTGATTGGCCCTGACCTCAGCAACTACAGTGAAGATGAGGC
CTGGCCAAGTCTCTTTGACACCTTTGTGGAGTGGGAAATGGAGCGAAGGA
AAAGAGAAGGGGAAGGGAGAGGTGCACTCAGCTCAGGGCCTGAGGGCTTG
TGTCCCGAGGAGCAGACTTAGGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMGQCVTKCKNPSS
TLGSKNGDREPSNKSHSRRGAGHREEQVPPCGKPGGDILVNGTKKAEAAT
EACQLPTSSGDAGRESKSNAEESSLQRLEELFRRYKDEREDAILEEGMER
FCNDLCVDPTEFRVLLLAWKFQAATMCKFTRKEFFDGCKAISADSIDGIC
ARFPSLLTEAKQEDKFKDLYRFTFQFGLDSEEGQRSLHREIAIALWKLVF
TQNNPPVLDQWLNFLTENPSGIKGISRDTWNMFLNFTQVIGPDLSNYSED
EAWPSLFDTFVEWEMERRKREGEGRGALSSGPEGLCPEEQT*


Vector Type
Mammalian
Antibiotic
Amp
Comments
*carries a94c mutation (silent Arg32)
Mammalian expression plasmid to express DCUN1D3 with N-terminal GST (contains precission protease site)

Estimated MW of expressed protein : 61726 Daltons
100042 R 1000 1001 1047 19007
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